Gene Information

Name : pyk (RBAM_026230)
Accession : YP_001422212.1
Strain : Bacillus amyloliquefaciens FZB42
Genome accession: NC_009725
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2748720 - 2750477 bp
Length : 1758 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
TTGAAAAAAACAAAAATCGTTTGTACAATCGGTCCGGCCAGTGAAAGTGTAGAAATGCTGACAAAATTGATGGAAGCGGG
TATGAACGTTGCTCGTCTGAATTTTTCACACGGTGATTTCGAAGAGCACGGCGCAAGAATCAAAAACATCAGAGAAGCAA
GCAAAAAGCTCGGCAAAAACGTGGGGATTCTCCTCGATACGAAAGGTCCGGAAATCCGCACGCACGATATGGAAAACGGC
GCGCTTGAGCTTCAGGCGGGAAATGAAATTATCGTTTCTACGAAACAGGTGCTGGGAACACTTGGAAAATTCTCAGTTTC
TTATGAAGGCCTGGCAGACGACGTGTCAGCAGGGTCTATCATCCTGTTGGATGACGGTTTAATCGGATTGGAAGTGCTAG
AATCAAATCCGGAAAAACACGAAATCAAAACAAAAATCTTAAACAGCGGCACGCTGAAAAATAAAAAAGGCGTAAACGTT
CCCGGCGTCAGCGTCAACCTTCCCGGAATTACTGAAAAAGACGCCAAAGACATCGTATTCGGTATCGAGCAAGGCGTTGA
TTTCATCGCTGCGTCATTTGTACGCCGTTCTACGGATGTCCTGGAAATCCGCGAACTGCTTGAAGAGCATAACGCGTCTG
ACATTCAAATCATTCCTAAGATCGAGAACCAGGAAGGCGTGGACAACCTGGATGCGATCCTGGAAGTATCTGACGGCTTA
ATGGTAGCGCGCGGAGACCTCGGCGTAGAGATTCCGGCCGAAGAAGTGCCGCTCGTGCAAAAAGAAATGATCAAAAAATG
CAACGCGCTCGGCAAACCGGTTATTACTGCGACACAAATGCTTGACAGCATGCAGCGCAACCCTCGTCCGACACGCGCTG
AAGCAAGTGACGTTGCCAACGCCATTTTCGACGGCACAGATGCTATCATGCTTTCCGGTGAGACGGCTGCGGGACAATAT
CCTGTCGAGGCGGTTCAAACAATGTTTAACATCGCCACACGCACGGAAGAATCTCTGAACTACAAAGAAATTCTGTCTAA
ACGCAGAGATCAGGTCGGCATGACGATTACCGATGCGATCGGCCAATCAGTTGCGCACACGGCGATCAATCTGAGTGCGG
CAGCTATCGTTACGCCTACAGAAAGCGGCCATACGGCGCGTATGATCGCGAAATACCGTCCGCAGGCTCCGATTGTAGCC
GTTACGGTTAATGAGTCTGTGTCACGCAAACTCGGCCTTGTATTCGGTGTATTCCCGTCAAGCGGCCAAAACGCGAATTC
AACCGATGAAATGCTTGAAGACGCGGTTCAAAAATCACTTGACAGCGGCATCGTGAAACGCGGTGATCTGATTGTCATTA
CAGCAGGTTCTGTCGGTGAATCAGGCACGACAAATCTGATGAAAGTCCACACTGTCGGCAGCATTATCGCGAAAGGGCAA
GGCATCGGGCGCAAATCTGCATTCGGTCCGGTCGTTGTTGCAAAAAATGCGAAAGAAGCTGAACAAAAAATGACAGACGG
CGCGGTATTGGTTGCCTCAAGCACTGACCGCGACATGATCGCTTCACTTGAAAAAGCATCAGCGCTCATCACTGAAGAAG
GCGGTCTGACAAGCCATGCAGCCGTTGTCGGTTTAAGCTTGGGCATTCCGGTTATCGTCGGTCTGGAGAACGCAACTTCA
GTTTTAACAGAAGGACAGGTTATCACAGTTGACGCTGCCCGCGGCGCTGTGTATGAAGGCCGCGCAAGCGTTCTTTAA

Protein sequence :
MKKTKIVCTIGPASESVEMLTKLMEAGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHDMENG
ALELQAGNEIIVSTKQVLGTLGKFSVSYEGLADDVSAGSIILLDDGLIGLEVLESNPEKHEIKTKILNSGTLKNKKGVNV
PGVSVNLPGITEKDAKDIVFGIEQGVDFIAASFVRRSTDVLEIRELLEEHNASDIQIIPKIENQEGVDNLDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKEMIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGQY
PVEAVQTMFNIATRTEESLNYKEILSKRRDQVGMTITDAIGQSVAHTAINLSAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNESVSRKLGLVFGVFPSSGQNANSTDEMLEDAVQKSLDSGIVKRGDLIVITAGSVGESGTTNLMKVHTVGSIIAKGQ
GIGRKSAFGPVVVAKNAKEAEQKMTDGAVLVASSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
VLTEGQVITVDAARGAVYEGRASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-107 54
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-107 54
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-107 54
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 3e-107 54

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_001422212.1 pyruvate kinase VFG0479 Protein 5e-108 54