Gene Information

Name : dhaS (RBAM_019060)
Accession : YP_001421499.1
Strain : Bacillus amyloliquefaciens FZB42
Genome accession: NC_009725
Putative virulence/resistance : Virulence
Product : DhaS
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1012
EC number : -
Position : 2022630 - 2024117 bp
Length : 1488 bp
Strand : +
Note : indol 3-acet-aldehyde dehydrogenase; involved in Trp dependent IAA synthesis. Mutant dhaS was found impaired in IAA synthesis and plant growth promotion

DNA sequence :
ATGAGTTCTTTAACGATGCAGATCACAAAAAGGCTGGAAACATTTTTACAGGGTACGAAGAAACTTTATATTGACGGAAA
ATTTGTTCCGAGCACTTCAGGATCAACATTTGTCACACCGAATCCGGCAACCGGAGAAACGCTGATGACGCTTTACGAGG
CACAGTCAGAAGACATTGACAGTGCAGTGAAAGCGGCACGCAAAGCCTTTGATCACGGCGAATGGCGCACTATGCCGGCC
GCTTCCAGAAGCAGGCTGATGTATAAGCTTGCCGATTTAATGGAAGAGCACAAAACGGAACTCGCGCAGCTTGAAACACT
TGATAACGGAAAACCGATTAATGAAACAACAAATGGGGATATCCCGCTCGCAATTGAGCATATGCGTTATTATGCCGGGT
GGTCTACAAAGATAACCGGACAAACGATTCCGGTCGCCGGCTCATATTTTAATTACACCCGCCATGAGCCTGTCGGCGTC
GTGGGGCAGATCATCCCTTGGAACTTCCCGCTCCTTATGGCGATGTGGAAAATGGGCGCCGCCCTTGCCACAGGCTGCAC
AATCGTTTTGAAACCGGCGGAACAGACACCGCTGACCGCCCTCTATTTAGCGGAGTTAATAGATCAGGCGGGCTTCCCTG
ACGGCGTCATCAATATTGTCCCAGGGTTCGGCGAGACGGCCGGGGAAGCGCTGACCAACCATGACGATGTCGATAAACTC
GCTTTTACCGGTTCTACAGAAATCGGCAAAAAAATCATGGAAAAAGCCGCAAAAAAAATTAAACGCGTGACACTGGAGCT
TGGCGGAAAGTCACCTAACATCATTTTGCCTGACGCCAATTTGAAGAAAGCCATTCCCGGCGCTTTAAACGGGGTCATGT
TTAATCAGGGGCAGGTATGCTGCGCAGGCTCCCGTGTCTTCATTCACAAAGACAAATATGATGAGGTCGTCAGCGAAATG
GTTTCATATGCGAAATCGCTTCAGCAAGGCGCCGGCCTTCACCAAGATACACAAATCGGCCCGCTCGTCAGTCAGGAACA
GCATGAGCGCGTGCTTTCATATATTGAAAAAGGAAAAGCGGAAGGAGCCAAAGCTGTCGCGGGCGGCAGCTGCCCGTTTG
AAGAAGGGTATTTTGTCTCCCCGACTGTATTTGCGGATGTGGATGACAGCATGACCATCGCAAAAGAAGAAATTTTCGGC
CCTGTTTTAGCCGCCATTCCGTATGATACGGTAGATGAAGTCATTGAGCGGGCGAACCGCTCGGAATACGGCCTTGCAGC
CGGACTTTGGACTGAGAATGTGAAAAACGCCCATTACATTGCTGACCGTCTCCAAGCCGGCACCGTTTGGGTCAACTGCT
ACAATGTTTTCGATGCGGCATCTCCTTTCGGCGGCTATAAGCAATCCGGCCTCGGGCGGGAAATGGGCTCTTACGCGCTG
AATAATTATACAGAAGTGAAAAGCGTCTGGATCAATCTGGAAGATTAA

Protein sequence :
MSSLTMQITKRLETFLQGTKKLYIDGKFVPSTSGSTFVTPNPATGETLMTLYEAQSEDIDSAVKAARKAFDHGEWRTMPA
ASRSRLMYKLADLMEEHKTELAQLETLDNGKPINETTNGDIPLAIEHMRYYAGWSTKITGQTIPVAGSYFNYTRHEPVGV
VGQIIPWNFPLLMAMWKMGAALATGCTIVLKPAEQTPLTALYLAELIDQAGFPDGVINIVPGFGETAGEALTNHDDVDKL
AFTGSTEIGKKIMEKAAKKIKRVTLELGGKSPNIILPDANLKKAIPGALNGVMFNQGQVCCAGSRVFIHKDKYDEVVSEM
VSYAKSLQQGAGLHQDTQIGPLVSQEQHERVLSYIEKGKAEGAKAVAGGSCPFEEGYFVSPTVFADVDDSMTIAKEEIFG
PVLAAIPYDTVDEVIERANRSEYGLAAGLWTENVKNAHYIADRLQAGTVWVNCYNVFDAASPFGGYKQSGLGREMGSYAL
NNYTEVKSVWINLED

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF SG19 AAN62241.1 putative aldehyde dehydrogenase Not tested PAGI-3(SG) Protein 2e-86 47
VC0819 NP_230467.2 aldehyde dehydrogenase Not tested VPI-1 Protein 3e-82 45
aldA AAC12273.1 aldehyde dehydrogenase Virulence VPI Protein 2e-83 45
aldA AAK20747.1 aldehyde dehydrogenase Virulence VPI Protein 2e-83 45
aldA AAK20776.1 aldehyde dehydrogenase Virulence VPI Protein 2e-83 45
aldA-1 YP_001216300.1 aldehyde dehydrogenase Not tested VPI-1 Protein 4e-83 45
hpaE AAO17179.1 HpaE Not tested tcd island Protein 4e-70 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
dhaS YP_001421499.1 DhaS VFG0082 Protein 1e-82 45