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Name : lysS (Mboo_2331) Accession : YP_001405488.1 Strain : Methanoregula boonei 6A8 Genome accession: NC_009712 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 2398552 - 2400096 bp Length : 1545 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : GTGACCGATCCTGCTGATACCCTTGCATTCGACCAGACCCGGCTCGACAAAGTCCGGGCCCTGCGGGAGAAAGGCATCGA CCTCTATCCCCCCACCTTTGACCGGAAGAATACGATCGCGGAGATCAGGACAAAATTCTCAGAGATCACCCACGAGAAGA GTGCCGAGGGCGTTGTTACCGCAGGACGGCTCTCCATCATCCGCAACCATGGCAAGACCATCTTTGCAGATCTCGGGGAC GAAAGCGGCAAGATCCAGCTCTACATCAGGAAGGCAGATCTTGGGGACGAGGCATTCGACTTCTTTAACCAGTACATCGA GCGGGGCGATTTTGTCGGCGTGACCGGGCACGTCTTCCGGACAAAACTGGGCGAGATCACGATCTGGGTGGACAAAATCC AGCTCCTTACAAAAGCCGTCTGCCCGTTGCCCGAGAAGTTCCACGGCCTTACCGATGTGGAGAAACGCTACCGGCAGCGG TACGTGGACCTGATTGCAAACGAGGAGGTCCGGCAGAACTTCCGGAACCGGAGCCGGATCCTCTCTTCCATCCGGCGCTA CCTCTCCGAGCGGGACTTTCTCGAATTCGAAACCCCGATCCTCCAGCCGGTGTACGGCGGTGCGAACGCACGCCCGTTTA CCACGTTCCACAACTGCCTGGGCCAGAAGCTCTTCCTCCGGATCGCACCCGAGCTGTACTTAAAACGGCTCGTGGTCGGC GGCTTTGAGCGGATCTTTGAGATCGCACGGAACTTCCGTAACGAGGACATTGACACGCACCACAACCCCGAGTTCACCAT GGTAGAGATCTACTGGGCGTACCACGATGTGTATGACATGATGGACCTCACCGAGGATTTCCTGGCAACGCTCGTGCAGG AGGTGCACAACAAAACGGAGATCACGTTTGAGGGCACAAGTATCTCCTTTGCAAAACCCCTGCGCAGACTCTCGATGGCC GATGCGGTAAAGGAGTACGCCGGGATCGACATCTTTGCCCTGTCGGTGGACGAGCTCCGGGCCTTTGCAATCCAAAACAA GCTCAAGGAAGCCGAAAAACCCCAGACACAGCGCGAGTTCCTGGTGTACTTCTTCGAGAACATGGTGGAGGAAAAACTGA TCCAGCCTACCTTTATCTACGATTTTCCCGTGGAGAACTCGCCGCTTGCAAAACGCCACCGGACAAAAGAAGGATTCACT GAGCGCTTCGAGCTCTTCATCTACGGCATGGAACTGGCAAACGGTTTCTCCGAGCTCAACGACCCGCTCGACCAGAAGGA GCGCTTCGAGGCGCAGGACAAAAAACGCCAGCTCGGCGATCTTGAAGCCCAGATGATCGATTACGATTTCATCAACGCGC TCGGGTACGGCATGCCACCGACCGGGGGCGTGGGCATCGGGATCGACCGGCTCGTGATGCTTCTTACCGGCAACAATTCC ATCAAGGAAGTGATCCTCTTCCCGTCCATGAAGAGCGTTCAGGCAGATGGCGAGGAGGCCGCCGGGAAAACTTCCCCCGC GGCCGAACCGGCGCAGAAGAACTGA Protein sequence : MTDPADTLAFDQTRLDKVRALREKGIDLYPPTFDRKNTIAEIRTKFSEITHEKSAEGVVTAGRLSIIRNHGKTIFADLGD ESGKIQLYIRKADLGDEAFDFFNQYIERGDFVGVTGHVFRTKLGEITIWVDKIQLLTKAVCPLPEKFHGLTDVEKRYRQR YVDLIANEEVRQNFRNRSRILSSIRRYLSERDFLEFETPILQPVYGGANARPFTTFHNCLGQKLFLRIAPELYLKRLVVG GFERIFEIARNFRNEDIDTHHNPEFTMVEIYWAYHDVYDMMDLTEDFLATLVQEVHNKTEITFEGTSISFAKPLRRLSMA DAVKEYAGIDIFALSVDELRAFAIQNKLKEAEKPQTQREFLVYFFENMVEEKLIQPTFIYDFPVENSPLAKRHRTKEGFT ERFELFIYGMELANGFSELNDPLDQKERFEAQDKKRQLGDLEAQMIDYDFINALGYGMPPTGGVGIGIDRLVMLLTGNNS IKEVILFPSMKSVQADGEEAAGKTSPAAEPAQKN |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 3e-96 | 47 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001405488.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 2e-96 | 47 |
