Gene Information

Name : ureC (YpsIP31758_1079)
Accession : YP_001400060.1
Strain : Yersinia pseudotuberculosis IP 31758
Genome accession: NC_009708
Putative virulence/resistance : Virulence
Product : urease subunit alpha
Function : -
COG functional category : E : Amino acid transport and metabolism
COG ID : COG0804
EC number : 3.5.1.5
Position : 1282541 - 1284259 bp
Length : 1719 bp
Strand : +
Note : ureases catalyze the hydrolysis of urea into ammonia and carbon dioxide; in Yersinia enterocolitica the ammonia released plays a key role in bacterial survival by neutralizing acids when colonizing the gastric mucosa; the holoenzyme is composed of 3 ureC

DNA sequence :
ATGCCTCAAATTTCTCGGCAAGAATACGCGGGTCTATTTGGCCCAACAACTGGCGATAAAATCCGTTTGGGTGATACCAA
TCTGTTTATCGAAATCGAAAAAGACTTGCGTGGATATGGTGAAGAGTCGGTTTACGGTGGTGGGAAATCGCTGCGTGACG
GGATGGGGGCCAACAATAATCTGACCCGCGATAATGGTGTGCTTGACCTGGTGATTACCAACGTCACGATTGTCGATGCT
CGCTTAGGGGTGATTAAAGCTGACGTCGGTATCCGCGATGGCAAAATCGCCGGTATCGGTAAGAGCGGTAACCCCGGTGT
TATGGATGGGGTGACGCAGGGTATGGTGGTCGGTGTGAGCACCGATGCTATCTCTGGTGAGCATTTGATTCTTACTGCCG
CCGGTATCGATAGCCATATTCATCTCATCTCCCCGCAACAGGCCTATCATGCGCTGTCCAACGGTGTTGCAACATTCTTT
GGTGGGGGGATTGGTCCAACGGACGGTACTAACGGGACAACCGTAACACCAGGGCCGTGGAATATCCGTCAAATGCTGCG
TTCGATAGAGGGGTTACCTGTCAACGTTGGTATTTTGGGGAAAGGTAATTCATACGGGCGCGGCCCGTTGCTGGAACAGG
CTATTGCCGGTGTTGTGGGCTATAAAGTTCATGAAGACTGGGGCGCGACGGCGAATGCTTTGCGTCATGCGCTGCGGATG
GCGGATGAGGTGGATATTCAGGTTTCGGTTCATACCGATAGCCTGAATGAATGTGGTTATGTAGAAGATACTATCGATGC
GTTCGAAGGCCGCACGATCCACACGTTCCACACCGAAGGGGCGGGTGGGGGCCATGCGCCGGATATTATTCGGGTGGCCA
GCCAAACTAACGTACTGCCAAGTTCAACCAACCCCACCCTGCCGTATGGTGTTAACAGCCAGGCTGAGCTGTTTGACATG
ATTATGGTGTGTCACAACCTTAACCCGAACGTACCTGCTGACGTCTCCTTTGCCGAAAGCCGTGTGCGCCCAGAAACGAT
TGCTGCTGAAAACGTTCTGCACGATATGGGCGTTATCTCCATGTTCTCCAGTGACTCACAAGCCATGGGGCGAGTGGGGG
AGAACTGGTTGCGTATTTTGCAAACCGCGGATGCGATGAAAGCGGCTCGGGGCAAATTGCCGGAAGATGCGGCGGGTAAC
GATAATTTCCGCGTGCTGCGCTATGTGGCAAAAATCACCATTAACCCCGCGATTACACAGGGGGTTAGCCATGTTATCGG
ATCGGTTGAAGTGGGAAAAATGGCCGATCTGGTGTTATGGGATCCTCGCTTCTTTGGTGCGAAACCGAAGATGGTTATCA
AGGGTGGCATGATTAACTGGGCCGCGATGGGCGATCCAAATGCGTCACTGCCAACACCACAGCCAGTGTTCTACCGCCCA
ATGTTTGGCGCGATGGGCAAAACGCTACAAGACACTTGTGTCACTTTCGTTTCTCAGGCTGCACTTGATGATGGTGTGAA
AGAGAAAGCGGGGCTGGATCGCCAAGTGATAGCGGTTAAAAACTGCCGCACGATCTCCAAACGTGATCTGGTGCGTAATG
ACCAAACACCAAACATTGAAGTGGATCCTGAAACCTTTGCAGTAAAAGTGGATGGGGTACATGCCACCTGTGAGCCAATT
GCAACTGCGTCGATGAATCAGCGCTATTTCTTTGGCTAA

Protein sequence :
MPQISRQEYAGLFGPTTGDKIRLGDTNLFIEIEKDLRGYGEESVYGGGKSLRDGMGANNNLTRDNGVLDLVITNVTIVDA
RLGVIKADVGIRDGKIAGIGKSGNPGVMDGVTQGMVVGVSTDAISGEHLILTAAGIDSHIHLISPQQAYHALSNGVATFF
GGGIGPTDGTNGTTVTPGPWNIRQMLRSIEGLPVNVGILGKGNSYGRGPLLEQAIAGVVGYKVHEDWGATANALRHALRM
ADEVDIQVSVHTDSLNECGYVEDTIDAFEGRTIHTFHTEGAGGGHAPDIIRVASQTNVLPSSTNPTLPYGVNSQAELFDM
IMVCHNLNPNVPADVSFAESRVRPETIAAENVLHDMGVISMFSSDSQAMGRVGENWLRILQTADAMKAARGKLPEDAAGN
DNFRVLRYVAKITINPAITQGVSHVIGSVEVGKMADLVLWDPRFFGAKPKMVIKGGMINWAAMGDPNASLPTPQPVFYRP
MFGAMGKTLQDTCVTFVSQAALDDGVKEKAGLDRQVIAVKNCRTISKRDLVRNDQTPNIEVDPETFAVKVDGVHATCEPI
ATASMNQRYFFG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ureC NP_286680.1 urease subunit alpha Virulence TAI Protein 2e-152 58
ureC NP_287088.1 urease subunit alpha Not tested TAI Protein 2e-152 58
ureC YP_005684266.1 Urease alpha subunit Not tested PiCp 7 Protein 6e-140 57
ureC YP_005682174.1 Urease alpha subunit Not tested PiCp 7 Protein 3e-140 57
ureC YP_003784325.1 urease subunit alpha Not tested PiCp 7 Protein 3e-140 57
ureC YP_005686358.1 Urease alpha subunit Not tested PiCp 7 Protein 3e-140 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
ureC YP_001400060.1 urease subunit alpha VFG0270 Protein 5e-148 59