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Name : lysS (CLI_3727) Accession : YP_001392873.1 Strain : Clostridium botulinum Langeland Genome accession: NC_009699 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 3863520 - 3865034 bp Length : 1515 bp Strand : - Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : GTGTCAAAGGAAGATAATGTGATAAACAGTTTTGAAGAACAAGCAAATGAATTAATGAAGGAAAGATTCCAAAAGTTAAA GGAACTTCAATCTAATGGCAAAGATCCATTTGATGTTTATAAGGTTGAAAGAACCCATACTTCAAAAGAAGTAAAGGATA ATTATGAAGATCTAGAAGGAAAAACTGTTACAGTGGCAGGAAGACTTATGTCAAAAAGAGTTCATGGAAAAGCTGGATTT TCAGATATACATGATAGATATGGAAAAATTCAATTATACATAAAGATAAATGATGTAGGGGAAGAAAAACTTAAAGAATA TAAGACTTTTGATATAGGTGATATAATAAGTGTAACAGGAACAGTTTTTAAAACAAAAACAGGAGAAACATCAATACACA TAACAGATTTTCAATTAGTTTGTAAATCTTTAAGACCATTACCAGAAAAGTGGCATGGATTAAAAGATCCAGATTTAAGA TATAGACAAAGATATGTAGATTTAATAATAAACCAAGATGTTAGAGATACTTTTATAAAAAGAACAGCTATAATTAAGTC TATGAGAGAATTCTTGGATAATAGAGGATTTTTAGAAGTAGAAACACCAATATTATCACCAATAGCAGGCGGAGCAGCAG CTAAGCCTTTTATTACTCATCATAATGCTTTAGATATAGATATGTATCTTAGAATAGCTACTGAATTATATCTAAAAAGA TTAATAGTAGGTGGCTTTGAAAAAGTATATGAGATTGGTAAAAACTTTAGAAATGAAGGAATAGATATAAGACATAATCC AGAATTTACAGCTATAGAATTATATGAAGCTTATGCGGATTATAATGATATGATGGAAATCACAGAAAATATGATAGCTT ATATATGCGAAAAAGTTCTAGGTACAACTAAAGTTGAATATGAAGGAGCAGAAATAGATTTTACACCACCATGGAGAAGA TTAACTATGGTAGATGCTGTAAAGGAATATGCAGGAGTAGATTTTAACATTATAAAAAATGACATCGAGGCAAGAACAAT CGCAAAAGAAAAACATATAGAATTTAAAAAAGAATTAAAGGATTGCACAAAAGGTGATGTACTTATAGGATTATTTGAGG AATTCTGTGAAGACAAGTTAATGCAGCCTACTTTTATTTGTGATTATCCAGTAGAAAATTCACCTCTTACAAAGAAGAAA AGAGGAAATGAAGCATTTACTGAAAGATTTGAAGGCTTTGTATTTGGAAGAGAAGTATGTAACGCATATTCTGAATTAAA TGATTCTATAGTTCAAAAAGAAAGATTCATGCAACAATTAAAGGAAAGAGAATTAGGTGATGATGAAGCTTATATGATGG ATGACGACTTTATAACTTCATTAGAAGTAGGTATGCCACCGACAGGAGGATTAGGAATAGGTATTGATAGATTAATAATG TTCTTAACAGATACACATTCTATAAGAGATGTTATACTATTCCCAACTATGAAACCGCAACCTAATAATCAATAA Protein sequence : MSKEDNVINSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKDNYEDLEGKTVTVAGRLMSKRVHGKAGF SDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLR YRQRYVDLIINQDVRDTFIKRTAIIKSMREFLDNRGFLEVETPILSPIAGGAAAKPFITHHNALDIDMYLRIATELYLKR LIVGGFEKVYEIGKNFRNEGIDIRHNPEFTAIELYEAYADYNDMMEITENMIAYICEKVLGTTKVEYEGAEIDFTPPWRR LTMVDAVKEYAGVDFNIIKNDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKK RGNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM FLTDTHSIRDVILFPTMKPQPNNQ |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 2e-108 | 48 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001392873.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 1e-108 | 48 |
