Gene Information

Name : pykA (CLI_3556)
Accession : YP_001392728.1
Strain : Clostridium botulinum Langeland
Genome accession: NC_009699
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 3709897 - 3711654 bp
Length : 1758 bp
Strand : -
Note : identified by match to protein family HMM PF00224; match to protein family HMM PF00391; match to protein family HMM PF02887; match to protein family HMM TIGR01064

DNA sequence :
ATGCAAAAGACTAAAATGATATTTACAATAGGTCCTGCAAGCTCGACAGAAGAAATCGTATCAAAATTAATAGAAGCTGG
AATGAGTGTATCAAGACACAATTTTTCTCACGGATCACATCCAGAACACAAAGAAAGAATGATGATGATTAAGAAATTAA
GAGAAAAACATAACAAACACATAGCTATTATGTTAGACACCAAAGGACCAGAAATAAGAACAGGTAATTTTTCAGTGGAT
AAAGTGGAATTAAAAGAAGGGGCAGAGTTTACAATATATTGTAGAGAAGACATAGTAGGTGATGAAACAAAATGTTCTAT
TACTTATGATGAACTATATAAAGATGTAGTTAAAGGAAATAAAATACTGATAGATGATGGATTAGTAGAGCTAGAGGTTC
AGTCAGTAGAAGATAATAAAATCCATACAGTAGTTAAAAATTCAGGAACTGTTTCAAATCACAAGGGAGTAAATGTTCCA
GGAGTTTCAGTATCACTTCCAGCAGTTACTGAAAAAGATATAGAAGATTTAAAATTTGGATGTGAAGTAGGAGTAGATTT
AATAAGTGCATCTTTTATAAGAAAAGCCTCTGATGTATTAGCTATAAGAAAAATATTGGAAGAAAATGGTGGAAATGAAA
TACAGATAATCTCTAAGATAGAAAACCAAGAGGGCGTTGACAATATAGATGAGATAATAAAATTTTCTGATGGAATAATG
GTAGCTAGAGGAGATATGGGAGTCGAAATTCCTATAGAAGAAGTTCCAATAGTACAAAAAAGAATAATAGAAAAATGTAA
TAAGGCAGGTAAGCCTGTAATTACAGCAACTCAAATGTTAGATTCTATGATGAGAAACCCTAGACCAACAAGAGCTGAAG
CCTCAGACATAGCCAATGCAATATTTGATGGAACAGATGCCATAATGTTAAGTGGAGAATCAGCAAATGGAAAATATCCA
GTAGAAGCTGCAAGAACTATGTCTAGAATAGCTAAAACAGCAGAAGCAAAATTAAATTATGATGCTATATTAAATAAAAT
GAGAGAAAGTCATATATTAAATGTTCCTAATGCTATAAGCTTATCAGCGTGTACTACAGCATCAGAATTAAATGCTACAG
CTATAATCACAGCAACTCAAAGTGGGCATACTGCAAAAATGGTATCAAAATATAGACCACAATGCCCTATTATAGCAGTA
ACACCAAATGAAATAGTTGCTAGAAAGTTAGCATTAAATTGTGGAGTAGTACCACTATTAACGGAAACATTTAATTCTAC
AGATGAGCTTATAGATAAATCTGTTAATAAATCTTTAGAAGAAGGATATGTTAAAAATGGCGACTTAGTTGTTATAGCAG
GAGGAATACCAGTGTCTTACTCAGGAACAACTAATATGCTAAAAGTTCATATAGTGGGAGATATACTAGCCCAAGGAAGA
GGTTCAGGAACTAGACCAACTTATGGGAATGTAAAGATAGTAAAAAGCCCTGCAGAAGCGGAAAATATAGTTGAAAGAGA
TGACATATTAGTAGTTAAAAATTTAGATAGAAGATATATGACTATTTTAGATAGAGTATCAGGTATAGTGGCTGAAGAAG
GTGGAATAACATCACACTTAGCTATAGAATGTATAGCAAGAGATATTCCATTTATATGTAATGCAGGTGGAGCTATGGAT
GTTTTAAAAACAGGAACATATGTAACACTTGATACAGTAAGAGGAATAGTATACAACGGAAGAGCTAATATAGTTTAA

Protein sequence :
MQKTKMIFTIGPASSTEEIVSKLIEAGMSVSRHNFSHGSHPEHKERMMMIKKLREKHNKHIAIMLDTKGPEIRTGNFSVD
KVELKEGAEFTIYCREDIVGDETKCSITYDELYKDVVKGNKILIDDGLVELEVQSVEDNKIHTVVKNSGTVSNHKGVNVP
GVSVSLPAVTEKDIEDLKFGCEVGVDLISASFIRKASDVLAIRKILEENGGNEIQIISKIENQEGVDNIDEIIKFSDGIM
VARGDMGVEIPIEEVPIVQKRIIEKCNKAGKPVITATQMLDSMMRNPRPTRAEASDIANAIFDGTDAIMLSGESANGKYP
VEAARTMSRIAKTAEAKLNYDAILNKMRESHILNVPNAISLSACTTASELNATAIITATQSGHTAKMVSKYRPQCPIIAV
TPNEIVARKLALNCGVVPLLTETFNSTDELIDKSVNKSLEEGYVKNGDLVVIAGGIPVSYSGTTNMLKVHIVGDILAQGR
GSGTRPTYGNVKIVKSPAEAENIVERDDILVVKNLDRRYMTILDRVSGIVAEEGGITSHLAIECIARDIPFICNAGGAMD
VLKTGTYVTLDTVRGIVYNGRANIV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-104 51
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-104 51
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-104 51
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-103 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykA YP_001392728.1 pyruvate kinase VFG0479 Protein 9e-105 51