Gene Information

Name : polA-2 (CLI_3068)
Accession : YP_001392289.1
Strain : Clostridium botulinum Langeland
Genome accession: NC_009699
Putative virulence/resistance : Unknown
Product : DNA polymerase I
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0749
EC number : 2.7.7.7
Position : 3244276 - 3246903 bp
Length : 2628 bp
Strand : -
Note : has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair

DNA sequence :
ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC
TGGAGAGGGAATTCATACTAATGCTATATATGGTTTTGTTAATATGCTTTTAAAAATGAAAGAAGAGATAAACCCAGATT
ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACCTTTAGACATGAAGAATATAAGGACTATAAAGCAGGTAGAAAAAAG
ATGCCAGAGGAACTTGCACAGCAGTTTCCTATAATAAAAGAGCTTTTGTCCGCATTAGCCATAAATATATTCGAAATTGA
TGGATTTGAAGCAGATGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG
GAGATAAAGATGCCCTACAATTGGCTACGGATAGCGTAAAAGTAGTTATAACTAAAAAGGGTATTACAGAAAAAGAAATT
TATGATAAAAATAGAATGGTAGAGGAGTTTGGAGTTACTCCTAAAGAATTTATAGATGTAAAAGGTCTTATGGGAGACAC
ATCTGACAATATACCTGGAGTACCTGGAATAGGTGAAAAAACTGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA
CAGTATTTGAAAACATAGAGAATATAAAAGGAAAAAAATTAAAGGAAAATTTACATGAATATAGAGAGCAGGCTATATTT
AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA
TATAAACAAAGTTAGAGAAATGTTTGAAAGGTTAGAATTTAAATCTTTAATGAATAAAGTAGGTAAAGGTAATGAGGGAG
AAACTGTAGAAGAGGAAGTCTCTTATAAAAATATAGTTTCTCTAGAAGAATTTAGAGATCTAAAAAATAATATAATAAAA
TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAGTAAAAACATTATA
TGCTAATTTTAAAGATGAGGTTTATAGAATAGACTTTGAAATGTTGCTAAATAATACTAAAGAACAATTTATAGAAGTAT
GCAGGGAAATGTTTGAGAGTAAAGAAATTAAAAAAATTACCTATGATGCAAAAAATCCAAGGACTATACTAAGAAAATTA
GGAATAGAGTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATATCTAATAAATCCAGTGAGGAAAGAATATGAGAT
ATCTAGTTTAGCTTCGGAATATTTGTTTAAAAATATAAATAGAGAAGATGAATTTTTAAAAATAAAAGAAGTTAATATAA
TGCCTAATTTATATAAAGTGCTGGAAGAAAAAATAAAAGAGCAAGATATGGAAGAACTGTTATATAAGGTAGAACAACCT
CTTACAGAAACATTATCAGCTATGGAGACAGAAGGTTTTAAAGTTGATAAAGATATACTTTTACAGCTAGAGAAAAAATT
TAAAGGGGAAATAGAAAGTACTCAGCTAGAAATATATTCAATGTCTGAAGAAGAATTTAATATAAATTCTCCTAAACAGT
TAGGGAAAATACTCTTTGAAAAATTAGATTTACCCGTTATAAAGAAAACAAAGACAGGATATTCTACTAATGCAGAGGTA
CTGGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAATTAACAAAAATATACTCTACTTA
TATAGAAGGGCTAAAAGCTGCTATAGATGAGGATGGGAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGGA
GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATC
CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA
TAAAAATATGATAAGTGCCTTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG
AAGAAGTAACACCTCTTATGAGAAGTAACGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA
TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT
TTATTTAGAAAATATAGTAGAAGAAGCCGTTGAAAAAGGGTATGTAAGTACTATATTAAATAGAAGAAGATATATAAAAG
AGGTTAAGTCTTCAAATAAAATAGTTAAAGCTGCAGGAGAAAGGCTAGCTATGAATAGTCCAATACAAGGAAGTGCAGCA
GATATAATAAAATTAGCTATGGTAAATGTTCATAGAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT
ACATGATGAATTAATATTAAATGTCCATAAAGATGAACTAGAAAAAATCAAAGCTTTAGTAAAAAAAGAAATGGAACAGG
TTTTAGAATTAAAAGTTGGGTTAGACGTGGATATAAACATAGGTAATAATTGGTATGAAGCAAAGTAG

Protein sequence :
MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK
MPEELAQQFPIIKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDSVKVVITKKGITEKEI
YDKNRMVEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVFENIENIKGKKLKENLHEYREQAIF
SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKGNEGETVEEEVSYKNIVSLEEFRDLKNNIIK
YKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFEMLLNNTKEQFIEVCREMFESKEIKKITYDAKNPRTILRKL
GIEFNGIKFDINLAAYLINPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP
LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV
LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI
PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRSNAKAVNFGIVYGIGDFSL
SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAVEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA
DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
polA YP_281523.1 DNA polymerase I Not tested Not named Protein 3e-169 45