Gene Information

Name : polA (CLC_2911)
Accession : YP_001388741.1
Strain : Clostridium botulinum Hall
Genome accession: NC_009698
Putative virulence/resistance : Unknown
Product : DNA polymerase I
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0749
EC number : 2.7.7.7
Position : 3073783 - 3076410 bp
Length : 2628 bp
Strand : -
Note : has 3'-5' exonuclease, 5'-3' exonuclease and 5'-3'polymerase activities, primarily functions to fill gaps during DNA replication and repair

DNA sequence :
ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC
TGGAGAGGGAATTCATACTAATGCTATATATGGTTTTGTTAATATGCTTTTAAAAATGAAAGAAGAGATAAACCCAGATT
ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACCTTTAGACATGAAGAATATAAGGACTACAAAGCAGGTAGAAAAAAG
ATGCCAGAGGAACTTGCACAGCAGTTTCCTATAGTAAAAGAGCTTTTGTCCGCATTAGCCATAAATATATTCGAAATTGA
TGGGTTTGAAGCAGACGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG
GAGATAAAGATGCCCTACAATTGGCTACAGATAATGTAAAAGTAGTTATAACTAAAAAGGGTATTACAGAAAAAGAAATT
TATGATAAAAATAGAATGGTAGAGGAGTTTGGAGTTACTCCTAAAGAATTTATAGATGTAAAAGGTCTTATGGGAGACAC
ATCTGACAATATACCTGGAGTACCTGGAATAGGTGAAAAAACTGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA
CAGTACTTGAAAACATAGAGAATATAAAAGGAAAAAAATTAAAGGAAAATTTACATGAATATAGAGAACAGGCTATATTT
AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA
TATAAACAAAGTTAGAGAAATGTTTGAAAGGTTAGAATTTAAGTCTTTAATGAATAAAGTAGGTAAAAGTGATGAGGAAG
AAACGGTAGAAGAGGAAGTTTCTTATAAAAATATAGTTTCTTTAGAAGAATTTAAAGATCTAAAAAATAATATAATAAAA
TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAGTAAAAACATTATA
TGCTAATTTTAAAGATGAAGTTTATAGAATAGACTTTGAAATGCTTATAAAAAATACTAAAGAACAATTTATAGAAGTAT
GTAAAGAAATATTTGAAAGTAAAGAAATTAAAAAAATTACCTATGATGCAAAAAATCCAAGGACTATATTAAGAAAGTTA
GGAATAGAGTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATATCTAATAGATCCAGTGAGGAAAGAATATGAGAT
ATCTAGTTTAGCTTCAGAATACTTGTTTAAAAATATAAACAGAGAAGATGAATTTTTAAAAATAAAAGAAGTTAATATAA
TGCCTAATTTATATAAAGTGCTGGAAGAAAAAATAAAAGAGCAAGATATGGAAGAATTGCTATATAAGGTAGAACAACCT
CTTACAGAAACATTATCAGCTATGGAGACAGAAGGTTTTAAAGTTGATAAAGATATACTTTTACAGCTAGAGAAAAAATT
TAAAGGGGAAATAGAAAGTACTCAGCTAGAAATATATTCAATGTCTGAAGAAGAATTTAATATAAATTCTCCTAAACAGT
TAGGGAAAATACTCTTTGAAAAATTAGATTTACCCGTTATAAAGAAAACAAAGACAGGATATTCTACTAACGCAGAGGTA
CTGGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAGTTAACAAAAATATACTCTACTTA
TATAGAAGGGCTAAAAGCTGCTATAGATGAGGATGGAAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGAA
GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATC
CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA
TAAAAATATGATAAGTGCCTTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG
AAGAAGTAACACCTCTTATGAGAGGTAATGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA
TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT
TTATTTAGAAAATATAGTAGAAGAAGCTATTGAAAAAGGTTATGTAAGTACTATATTAAATAGAAGAAGATACATAAAAG
AGGTTAAGTCCTCAAATAAAATAGTTAAAGCTGCAGGAGAAAGGCTAGCTATGAATAGTCCAATACAAGGAAGTGCAGCG
GATATAATAAAATTAGCTATGGTAAATGTTCATAGAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT
ACATGATGAATTAATATTAAATGTCCATAAAGATGAACTAGAAAAAATCAAAGCTTTAGTAAAAAAAGAAATGGAACAGG
TTTTAGAATTAAAAGTTGGGTTAGACGTGGATATAAACATAGGTAATAATTGGTATGAGGCAAAGTAG

Protein sequence :
MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK
MPEELAQQFPIVKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDNVKVVITKKGITEKEI
YDKNRMVEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVLENIENIKGKKLKENLHEYREQAIF
SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKSDEEETVEEEVSYKNIVSLEEFKDLKNNIIK
YKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFEMLIKNTKEQFIEVCKEIFESKEIKKITYDAKNPRTILRKL
GIEFNGIKFDINLAAYLIDPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP
LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV
LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI
PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRGNAKAVNFGIVYGIGDFSL
SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAIEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA
DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
polA YP_281523.1 DNA polymerase I Not tested Not named Protein 7e-166 45