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Name : lysS (CLB_3593) Accession : YP_001385834.1 Strain : Clostridium botulinum ATCC 19397 Genome accession: NC_009697 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 3736722 - 3738236 bp Length : 1515 bp Strand : - Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : GTGTCAAAGGAAGATAACGTGATGAACAGTTTTGAAGAACAAGCAAATGAATTAATGAAGGAAAGATTTCAAAAGTTAAA GGAACTTCAATCTAATGGCAAAGATCCGTTTGATGTTTATAAGGTTGAAAGAACCCATACTTCAAAAGAAGTAAAGGAAA ACTATGAAGATCTAGAAGGAAAAACTGTTACAGTAGCAGGAAGACTTATGTCAAAAAGAGTTCATGGAAAAGCTGGATTT TCAGATATACATGATAGATATGGAAAAATTCAATTATACATAAAGATAAATGATGTAGGAGAAGAAAAACTTAAAGAATA TAAGACTTTTGATATAGGTGATATAATAAGTGTAACAGGAACAGTTTTTAAAACAAAAACAGGAGAAACATCAATACACA TAACAGATTTTCAGTTAGTTTGTAAATCTTTAAGACCATTACCAGAAAAGTGGCATGGATTAAAAGATCCAGATTTAAGA TATAGACAAAGATATGTAGATTTAATAATAAACCAAGATGTTAGAGATACTTTTATGAAGAGAACAGCCATAATTAAAAC CATGAGGGAGTATTTGGATAATAAGGGATTTTTAGAAGTAGAAACACCAATATTATCACCAATAGCAGGGGGAGCAGCAG CTAAACCATTTATTACTCATCATAATGCTTTAAATATAGATATGTATCTTAGAATAGCTACTGAATTATATTTAAAAAGA TTAATAGTAGGTGGATTTGAAAAAGTATATGAAATTGGTAGAAACTTTAGAAACGAAGGAATGGATATAAGACATAATCC AGAATTTACAGTTATAGAATTATATGAAGCTTATGCAGATTACAATGATATGATGGAAATAACAGAAAATATGATAGCAT ATATATGTGAAAAAGTTCTAGGTACAACTAAAGTTCAATATGAAGGAACAGAAATAGATTTTACACCACCATGGAGAAGA TTAACTATGGTAGATGCTGTAAGGGAATATGCAGGAGTAGATTTTAACACTATAAAAGATGACATCGAGGCAAGAACAAT CGCAAAAGAAAAACATATAGAGTTTAAAAAAGAATTAAAGGATTGCACAAAAGGTGATGTACTTATAGGATTATTTGAGG AATTCTGTGAAGATAAGTTAATGCAACCTACTTTTATTTGTGATTATCCAGTAGAGAATTCACCTCTTACAAAGAAGAAA AGAGGAAATGAAGCATTTACTGAAAGATTTGAAGGTTTTGTATTTGGAAGAGAGGTATGTAACGCATATTCTGAATTAAA TGATTCTATAGTTCAAAAAGAAAGATTCATGCAACAATTAAAGGAAAGAGAATTAGGTGATGATGAAGCCTATATGATGG ATGACGACTTTATAACTTCATTAGAAGTAGGTATGCCACCAACAGGAGGATTAGGAATAGGTATTGATAGATTAATAATG TTCTTAACAGATACACATTCTATAAGAGATGTTATACTATTCCCAACTATGAAACCACAACCTAATAATCAATAA Protein sequence : MSKEDNVMNSFEEQANELMKERFQKLKELQSNGKDPFDVYKVERTHTSKEVKENYEDLEGKTVTVAGRLMSKRVHGKAGF SDIHDRYGKIQLYIKINDVGEEKLKEYKTFDIGDIISVTGTVFKTKTGETSIHITDFQLVCKSLRPLPEKWHGLKDPDLR YRQRYVDLIINQDVRDTFMKRTAIIKTMREYLDNKGFLEVETPILSPIAGGAAAKPFITHHNALNIDMYLRIATELYLKR LIVGGFEKVYEIGRNFRNEGMDIRHNPEFTVIELYEAYADYNDMMEITENMIAYICEKVLGTTKVQYEGTEIDFTPPWRR LTMVDAVREYAGVDFNTIKDDIEARTIAKEKHIEFKKELKDCTKGDVLIGLFEEFCEDKLMQPTFICDYPVENSPLTKKK RGNEAFTERFEGFVFGREVCNAYSELNDSIVQKERFMQQLKERELGDDEAYMMDDDFITSLEVGMPPTGGLGIGIDRLIM FLTDTHSIRDVILFPTMKPQPNNQ |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 5e-109 | 49 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001385834.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 3e-109 | 49 |
