Gene Information

Name : lysS (Bcer98_0072)
Accession : YP_001373442.1
Strain : Bacillus cytotoxicus NVH 391-98
Genome accession: NC_009674
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 80869 - 82368 bp
Length : 1500 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGATAACATGAACCATGAAGAATTAAACGACCAATTACTTGTTCGTCGTGAGAAACTACATAACTTACGTGAACAAGG
TATCGATCCATTTGGTAAACGTTTTGAACGTACACATTCATCAAAAGAGTTACTGAACTTGTATGGAGAGTTCTCTAAAG
AAGAATTAGAGGAAAAAGCAATTTCTGTTTCTATTGCTGGGCGCATTATGACAAAGCGCGGTAAAGGGAAAGCTGGTTTT
GCGCATATTCAAGATTTACATGGACAAGTTCAAATTTACGTTCGTAAGGATGCCGTTGGAGATGAAGAATATGAATTGTT
TAAAACGGCTGATTTAGGTGACTTAGTCGGAATTGAAGGGAAAGTTTTCAAAACAAATGTTGGAGAACTTTCTGTAAAAG
CAACATCATTTACTTTATTAACGAAATCTCTTCGTCCACTTCCGGATAAATACCATGGATTGAAAGATATTGAGCAACGT
TATCGTCAACGTTACTTAGATCTAATCACAAGTATGGAGAGTCGCGAAACATTTGTTACTCGCAGTAAAATCATCCGTGA
AATGAGAAGATATCTGGATGATAATGGCTATCTTGAGGTAGAAACACCAATGATGCATACAATTGCAGGTGGTGCATCTG
CTCGTCCGTTCATTACGCATCATAATGCATTAGATATGGAATTATATATGCGTATTGCAATTGAGTTGCATTTAAAACGT
CTTATTGTAGGCGGCCTAGAAAAAGTATATGAAATTGGCCGTGTTTTCCGTAATGAAGGTGTATCAACACGTCATAATCC
AGAATTTACAATGATTGAATTATATGAAGCGTATGCTGACTATAAAGATATTATGAAACTAACAGAAAATATGATTGCTC
ATATTGCGAAGCGAGTGTTGGGTACAACAACAATCCAATATGGTGAACATGAAATTAATTTAGAACCAGAATGGACGCGT
CTTCATATGGTAGATGCAATTAAACAATATTGTGGAGTGGACTTCTGGAAACCAATGAGCGTGGAAGAAGCGCGTGCATT
AGCGAAAGAACATGATGTAGAAATTAAAGATACAATGGAAGTTGGTCACATCATTAACGAATTCTTTGAACAAAAAGTAG
AAGAAAAGTTAATTCAACCTACATTTATCTACGGGCATCCAGTAGAAATTTCGCCTCTTGCGAAAAAGAACGATGAGGAT
TCACGATTTACAGATCGTTTTGAACTATTCATTGTTGCACGCGAACATGCAAATGCATTTACTGAATTAAATGATCCAAT
CGATCAGAAAGAACGTTTTGAAGCTCAATTAAAAGAGCGTGAACAAGGAAATGATGAGGCGCATATGATGGACGATGATT
ATATCGAAGCTCTTGAATATGGTATGCCTCCTACTGGTGGATTAGGTATTGGTATTGATCGCCTTGTTATGCTATTAACA
AATTCACCATCTATCCGTGATGTATTGTTATTCCCTACAATGCGTCATAAACAAGACTAA

Protein sequence :
MDNMNHEELNDQLLVRREKLHNLREQGIDPFGKRFERTHSSKELLNLYGEFSKEELEEKAISVSIAGRIMTKRGKGKAGF
AHIQDLHGQVQIYVRKDAVGDEEYELFKTADLGDLVGIEGKVFKTNVGELSVKATSFTLLTKSLRPLPDKYHGLKDIEQR
YRQRYLDLITSMESRETFVTRSKIIREMRRYLDDNGYLEVETPMMHTIAGGASARPFITHHNALDMELYMRIAIELHLKR
LIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYKDIMKLTENMIAHIAKRVLGTTTIQYGEHEINLEPEWTR
LHMVDAIKQYCGVDFWKPMSVEEARALAKEHDVEIKDTMEVGHIINEFFEQKVEEKLIQPTFIYGHPVEISPLAKKNDED
SRFTDRFELFIVAREHANAFTELNDPIDQKERFEAQLKEREQGNDEAHMMDDDYIEALEYGMPPTGGLGIGIDRLVMLLT
NSPSIRDVLLFPTMRHKQD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 9e-126 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001373442.1 lysyl-tRNA synthetase VFG1668 Protein 5e-126 51