Gene Information

Name : Krad_1653 (Krad_1653)
Accession : YP_001361403.1
Strain : Kineococcus radiotolerans SRS30216
Genome accession: NC_009664
Putative virulence/resistance : Virulence
Product : FliI/YscN family ATPase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.15
Position : 549545 - 550906 bp
Length : 1362 bp
Strand : -
Note : KEGG: ace:Acel_0844 flagellar protein export ATPase FliI; TIGRFAM: ATPase, FliI/YscN family; PFAM: H+transporting two-sector ATPase alpha/beta subunit central region; SMART: AAA ATPase

DNA sequence :
GTGAGCGCCCAGCTCGTCTCCCACCGCCTGGCGGCCGGCGTGCGCGAGATCCTCGCGCCGCAGGTCGCCCGGGCCGTCGA
GGCGGCCCGTCCCGAGGTCCGCGGGCGGGTCTCCGGCATCGTCGGCCTCTCCGTGGAGGTCTCCGGCCTGGAGGCCGCGG
TGGGGGAGACCGTCCGCCTCGGCGACGAGCGGTACTCCATCCCCGCCGAGGTCGTCGCCGCGGAGGGGTCGCGCCTGCGC
TGCCTGCCGCTGGGTCACCTCACCGGCCTGGCCGCCGGCGCCCCCGCCGTGGCGGTCCGGCGCCCCTCCGACGTCCCCGT
CGGCCCCGCGCTGCTGGGCCGCGTCGTCGACGCGCTGGGCAACCCCCTCGACGGGGGCCCGCCCCTGCACACCGCCCCCC
GCGGCCCCCTGCACGCCACCCCGCCCCCGGCCATGACCCGCGGGCGCGTCGACGCCCCCCTCAGCCTCGGCGTGCGGGCC
CTGGACACCCTGGTCCCCGCCGGGCGCGGGCAGCGCTTCGGCATCTTCGCCGGCTCCGGCGTCGGCAAGTCCTCCCTGCT
GTCGATGATCGCCCGGGGCACCTCCGCCGACGTGTCGGTGCTGGCCCTGGTCGGCGAGCGCGGGCGGGAGGTCCGCGAGT
TCCTCGAGGACGACCTCGGCCCCGAGGGCCTGGCCCGCTCCGTCGTCGTCATCGCCACCTCCGACGAGGCCCCCGTGCTG
CGCCTGCGCGCCTCGCTGACGGCGACCACCGTCGCGGAGTCCTTCCGCGACATGGGCGCCGACTGCGTCCTCATGATGGA
CTCCCTCACCCGCGTCGCCATGGCCCAGCGCGAGATCGGCCTGTCCGTGGGCGAACCCCCCGCCACCCGCGGCTACCCGC
CGAGCGTCTTCGCGCTCATGCCGCGGCTGCTGGAACGGGCCGGGCCCGGGGTCACCGGCTCCATCACCGGGATCTACACC
GTCCTCGTCGACGGCGACGACCACAACGAGCCCATCGCCGACAACGCGCGCTCCATCCTCGACGGCCACGTCGTCCTGGA
CCGCCGCCTGGCCACGGCCGGGCACTTCCCGACCATCGACGTCCTGGAGTCGGTCTCCCGCGTCGCCGGCCGCGTCACCA
CGCCCGAGCAGAAGAGCGCCGCCACGGCGCTGCGCCGGGTCATGGCCGCCCACCGCGACGCCAAGGAGCTCATCGACGTC
GGCGCCTACCAGCCCGGCGCCAACCCCGAGGTCGACGCCGCGGTGGCCCAGCTGCCCGCGATCAACGCCTTCCTGCGCCA
GGACATCCACGACATCACCCCCGCCCCGCAGGCCTGGGCGCAGCTGCAGCAACTGGTCTCGACCTTCGGAGGTGTGCGGT
GA

Protein sequence :
MSAQLVSHRLAAGVREILAPQVARAVEAARPEVRGRVSGIVGLSVEVSGLEAAVGETVRLGDERYSIPAEVVAAEGSRLR
CLPLGHLTGLAAGAPAVAVRRPSDVPVGPALLGRVVDALGNPLDGGPPLHTAPRGPLHATPPPAMTRGRVDAPLSLGVRA
LDTLVPAGRGQRFGIFAGSGVGKSSLLSMIARGTSADVSVLALVGERGREVREFLEDDLGPEGLARSVVVIATSDEAPVL
RLRASLTATTVAESFRDMGADCVLMMDSLTRVAMAQREIGLSVGEPPATRGYPPSVFALMPRLLERAGPGVTGSITGIYT
VLVDGDDHNEPIADNARSILDGHVVLDRRLATAGHFPTIDVLESVSRVAGRVTTPEQKSAATALRRVMAAHRDAKELIDV
GAYQPGANPEVDAAVAQLPAINAFLRQDIHDITPAPQAWAQLQQLVSTFGGVR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-82 56
lscN AAO18044.1 LscN Virulence TTSS locus Protein 7e-86 55
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-84 54
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-84 54
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-82 53
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-78 52
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 4e-79 52
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 8e-79 52
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-79 52
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 6e-79 52
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-82 52
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 9e-82 52
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 5e-82 52
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 5e-82 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-81 52
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-82 52
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 9e-73 51
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 9e-73 51
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-74 51
escN AAC31513.1 L0034 Virulence LEE Protein 1e-74 51
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-74 51
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-74 51
escN YP_003223475.1 translocator EscN Virulence LEE Protein 2e-74 51
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-74 51
escN YP_003236088.1 translocator EscN Virulence LEE Protein 5e-74 51
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 3e-74 51
escN AAK26715.1 EscN Virulence LEE Protein 9e-74 50
escN AAL57542.1 EscN Virulence LEE Protein 9e-74 50
escN CAC81862.1 EscN protein Virulence LEE II Protein 3e-74 50
escN AAC38383.1 EscN Virulence LEE Protein 2e-74 50
unnamed AAL06369.1 EscN Virulence LEE Protein 5e-74 50
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 8e-72 50
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 3e-67 50
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-67 50
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-67 50
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 9e-67 49
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 5e-63 48
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 5e-63 48
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 6e-64 48
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 4e-64 48
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-62 47
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-61 47
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 9e-61 47
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-61 47
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-61 47
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-60 47
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-60 47
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 2e-63 46
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-60 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG0192 Protein 8e-87 56
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG0048 Protein 9e-87 56
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG0390 Protein 4e-86 53
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG2331 Protein 1e-84 53
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG0515 Protein 2e-79 52
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG2144 Protein 4e-81 52
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG0812 Protein 8e-75 51
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG0730 Protein 1e-74 50
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG1254 Protein 2e-87 48
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG2459 Protein 4e-66 47
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG0555 Protein 4e-61 47
Krad_1653 YP_001361403.1 FliI/YscN family ATPase VFG1008 Protein 3e-60 42