Gene Information

Name : lysS (NIS_1177)
Accession : YP_001356643.1
Strain : Nitratiruptor sp. SB155-2
Genome accession: NC_009662
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1192472 - 1193980 bp
Length : 1509 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
TTGATATTTGACAACCAGTATGAACAGTTACGCATACAAAAAGCGCAAGAACTGAAAGAAGCAGGCATCAACCCTTATGG
ACACGGCTTTTGCAAAGAGATTTCCAATAATGAATTTTTAGAAAAATATGCGTATGTCAAAGATCTTGAAGAAAAGAAAG
ATGAAAAGGCGTGCGTAAAACTCAACGGCCGCATCAAATTTCTACGACATATGGGTAAGGCCGCTTTTGCAAAAATAGAA
GATGAAAGCGGGATCGTACAGATCTACTTCAATCGCGACTCCCTTGGCGATGAGTGGTTTAAAAAAGTAAAAAAATTGAT
TGAAGTCGGCGATATCATCGAAGCAAAAGGGTATCCCTTTGTAACAAAAACTGGTGAACTCACCCTCCATGCAAATGAAT
TAAACCTAGTGACAAAGGCTATCGTTCCCCTTCCTGAGAAATTTCATGGGCTACAAGACAAAGAGCTTCGATATAGACAG
CGCTATGTAGACCTTATCATGAATCCTGAGGTGAAGAAAGTTTTTATGCTTCGAAGCCGTATCGTTAGCCTCATTCGAGA
CTTCTTTGAAAGCCACGGCTTTTTGGAAGTGGAAACTCCTATGATGCATCCGATTCCGGGCGGTGCGAACGCGCGTCCTT
TCATTACCCACCACAATGCGTTAGGCGTAGATAGATACCTTCGAATCGCACCTGAACTCTATCTCAAACGGCTTATTGTC
GGGGGATTCGAAGCAGTCTTTGAAATCAACAGAAACTTTCGTAACGAAGGGATGGATGCTACCCACAACCCAGAATTCAC
GATGATCGAGTTTTACTGGGCATACCATACCTATGAAGATTTGATGAAACTCACTGAAGATCTTTTCGATTACCTTTTCG
AAAAGCTCTCGTTGCCCAAAAAGCTTACCTATGGTGAACATACGATCGATTTTTCCACACCGTTTAGCAAAATAAAATAT
AAAGATGCCATCATCGAAATCGGTGAAGTACCTGAAGATATAGTGGAAGATAAAGAAAAGATTATCAGCTTTTTGAAAGA
TAAAGGCATTGAACCAGAATTTGCACACAAAATGAGTCTTGGTGCTCTCCAGGCCGAACTTTTTGACAATTTCGTAGAAG
AAAAACTCATCAACCCAACATTTATCACCCACTTCCCGATAGATATCAGCCCGCTTGCTAGACGAAGTGATGAAAATCCT
GAGATTGCCGAAAGATTTGAGCTTTTCATCGCCGGCAAAGAGATCGCGAACGGCTTCAATGAGCTCAATGATCCACTGGA
TCAGTATGAGCGTTTCAAAGCGCAAGTACAAGCTAAAGAGGTGGATGACGAAGCGATGCATATGGATGAGGATTTTGTCC
GTGCCCTTGGATACGGTATGCCTCCAACTGCAGGGGAAGGCATCGGAATCGATAGACTCGTAATGTTATTAACGAACCAA
CATTCCATTAGAGATGTGATTTTATTTCCAGCTATGAAACCTATACAAAAAGAGGAAAAAGGAGAATAA

Protein sequence :
MIFDNQYEQLRIQKAQELKEAGINPYGHGFCKEISNNEFLEKYAYVKDLEEKKDEKACVKLNGRIKFLRHMGKAAFAKIE
DESGIVQIYFNRDSLGDEWFKKVKKLIEVGDIIEAKGYPFVTKTGELTLHANELNLVTKAIVPLPEKFHGLQDKELRYRQ
RYVDLIMNPEVKKVFMLRSRIVSLIRDFFESHGFLEVETPMMHPIPGGANARPFITHHNALGVDRYLRIAPELYLKRLIV
GGFEAVFEINRNFRNEGMDATHNPEFTMIEFYWAYHTYEDLMKLTEDLFDYLFEKLSLPKKLTYGEHTIDFSTPFSKIKY
KDAIIEIGEVPEDIVEDKEKIISFLKDKGIEPEFAHKMSLGALQAELFDNFVEEKLINPTFITHFPIDISPLARRSDENP
EIAERFELFIAGKEIANGFNELNDPLDQYERFKAQVQAKEVDDEAMHMDEDFVRALGYGMPPTAGEGIGIDRLVMLLTNQ
HSIRDVILFPAMKPIQKEEKGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-112 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001356643.1 lysyl-tRNA synthetase VFG1668 Protein 5e-113 51