Gene Information

Name : pyk1 (PSPA7_4898)
Accession : YP_001350234.1
Strain : Pseudomonas aeruginosa PA7
Genome accession: NC_009656
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 5051250 - 5052701 bp
Length : 1452 bp
Strand : +
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGTCCGTTCGCCGCACCAAAATCGTCGCCACCCTCGGCCCAGCCAGCAATTCCCCGGAAGTGCTGGAGCAGCTGATCCT
GGCCGGGATCGACGTCGCCCGCCTCAACTTCTCCCACGGCACCCCCGATGAACACCGTGCCCGCGCCAGGCTGGTGCGCG
AGCTGGCGGCCAGGCACGGCCGCTTCGTCGCCCTGCTCGGCGACCTGCAAGGACCGAAGATCCGCATCGCCAAGTTCGCC
AGCAAGCGCATCGAATTGCAGGTCGGCGACAGGTTCCGCTTCTCCACCAGCCACCCGCGCGACGCCGGCACCCAGGAAGT
GGTCGGCATCGACTATCCGGACCTGGTGAAGGACTGCGGTGTCGGCGACGAACTGCTGCTGGACGACGGCCGGGTGGTGA
TGGTGGTCGAGGAAGTCGCCGCCGACGAACTGCGCTGCCGGGTGCTGATCGGCGGCCCGCTGTCCGACCACAAGGGCATC
AACCGCCGCGGCGGCGGTCTCACCGCGCCGGCGCTGACCGACAAGGACAAGGCCGACATCAAGCTGGCCGCGGACATGGA
CCTGGACTACGTGGCCGTTTCCTTCCCGCGCGACGCCAAGGACATGGAATACGCCCGCAGCCTGCTCACCGAGGCCGGTG
GCAAGGCCTGGCTGGTGGCCAAGATCGAACGCGCCGAAGCGGTGGCCGACGACGACGCCCTCGACGGCCTGATCCGCGCC
AGCGACGCGGTGATGGTGGCGCGCGGCGACCTCGGCGTGGAAATCGGCGATGCCGAGCTGGTCGGCATCCAGAAGAAGAT
CATCCTGCACGCCCGCCGCAACAATAAAGTGGTGATCACCGCGACCCAGATGATGGAGTCGATGATCCACAGCCCGATGC
CGACCCGCGCGGAAGTCTCCGACGTGGCCAACGCCGTACTCGACTACACCGACGCGGTGATGCTCTCGGCGGAAAGCGCC
GCCGGCGAATACCCGGTGGAAGCGGTGAAAGCCATGGCCCGCGTCTGCCAGGGCGCCGAGAAGCACCCGACCAGCCAGAA
GTCCAGCCACCGCCTCGGCCAGACCTTCGACCGCTGCGACGAGAGCATCGCCCTGGCGTCGATGTATACCGCCAACCACT
TCCCGGGGATCAAGGCGATCATCTGCCTGACCGAAAGCGGCTTCACCCCGCTGATCATGTCGCGCATCCGCTCCTCGGTA
CCGATCTACGCCTATTCCCCGCACCGCGAGACCCAGGCCCGCGTGGCCATGTTCCGCGGCGTGGAGACCATCCCCTTCGA
CCCGGCCGCGCTGCCACCGGAAAAGGTCAGCCAGGCCGCGGTCGACGAACTGCTCAAGCGCGGCGTGGTGACCAAGGGCG
ACTGGGTGATCCTGACCAAGGGCGACAGCTATACCGCCCAGGGCGGGACCAACACCATGAAGGTCCTGCACGTCGGCGAC
CTGCTGGTATAA

Protein sequence :
MSVRRTKIVATLGPASNSPEVLEQLILAGIDVARLNFSHGTPDEHRARARLVRELAARHGRFVALLGDLQGPKIRIAKFA
SKRIELQVGDRFRFSTSHPRDAGTQEVVGIDYPDLVKDCGVGDELLLDDGRVVMVVEEVAADELRCRVLIGGPLSDHKGI
NRRGGGLTAPALTDKDKADIKLAADMDLDYVAVSFPRDAKDMEYARSLLTEAGGKAWLVAKIERAEAVADDDALDGLIRA
SDAVMVARGDLGVEIGDAELVGIQKKIILHARRNNKVVITATQMMESMIHSPMPTRAEVSDVANAVLDYTDAVMLSAESA
AGEYPVEAVKAMARVCQGAEKHPTSQKSSHRLGQTFDRCDESIALASMYTANHFPGIKAIICLTESGFTPLIMSRIRSSV
PIYAYSPHRETQARVAMFRGVETIPFDPAALPPEKVSQAAVDELLKRGVVTKGDWVILTKGDSYTAQGGTNTMKVLHVGD
LLV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 3e-63 45
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-46 43
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-46 43
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-46 43
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 7e-46 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk1 YP_001350234.1 pyruvate kinase VFG0479 Protein 6e-47 43