Gene Information

Name : cadA2 (PSPA7_1448)
Accession : YP_001346832.1
Strain : Pseudomonas aeruginosa PA7
Genome accession: NC_009656
Putative virulence/resistance : Resistance
Product : cadmium-translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : 3.6.3.-
Position : 1467062 - 1469308 bp
Length : 2247 bp
Strand : -
Note : identified by match to protein family HMM PF00122; match to protein family HMM PF00702; match to protein family HMM TIGR01494; match to protein family HMM TIGR01512; match to protein family HMM TIGR01525

DNA sequence :
ATGAGCCACGAACACGCCGACACCTGCTGTCACGGTCACGGTCACGGTCACGGTCACGGTCACGGTCACGGACATGATCA
TGGGCATCGCCACGCGCCCCGTCCCGCGCTGGCTGCCATCGGAACCCTGGCCGGCAACGCCGAGCTGCGCTGGAGCGACC
TGCGCATCGAAGCGATGGACTGCCCCACCGAAGAGCGCCTGATCCGCGATGCCCTCGGCCGCCAGCCGGCGGTGGAGTCG
CTGGAGTTCAACCTGATGCAGCGCCTGCTGCGGGTCCAGCATCGCTTCGACAGCGTCGAGCCGCTGCAGAAGCTGATCGC
CGGCCTCGGCATGCAGGCGGTGCCGCTGGACGCTGGCGAAGGGCTCGACGGGCCCTCGGAAGCGCCGACGAAACCCTGGT
GGCCATTGGCCCTGGCCGGCGTCATGGCGCTGGCCTCGGAAGTCGTCCACTGGTTCGGCCTGGCGCCGGACTGGGTGGTC
GCGGGCCTCGCCCTGCTGGCGATCCTCGGCGCCGGCCTGCCGACCTACCGCAAGGGCTGGATTGCCCTGAAGAACCGCAA
CCTGAACATCAACGCGCTGATGAGCATCGCCGTGACCGGTGCCGTGCTGATCGGCCAGTGGCCGGAAGCGGCGATGGTCA
GCGTGCTGTTCGCCATCGCCGAACTGATCGAGGCGAAGTCCCTGGATCGCGCGCGCAACGCCATCCGCGGTCTGCTGCAA
CTGGCTCCGGAACAGGCCACGGTGCTGGTCGGCGGCGAATGGAAGGAGTTGCCGGCCAAGCAAGTGGAGCTGGAAGCCAC
CGTGCGCGTGAAGCCCGGCGAACGCATCGCCCTCGATGGCGAGGTGATCAGCGGCCGCTCCAGCGTCAACCAGGCGCCGA
TCACCGGCGAAAGCCTGCCGGTGGAGAAGGAGGTCGGCGAGCCGCTGTTCGCCGGCAGCATCAACGGCGAGGGCGCGCTG
GAATACCGCGTCACCCGCCGCGCCGACGACAGCACCCTGGCACGGATCATCCACGCGGTGGAGGAAGCCCAGGGCTCCCG
CGCGCCGACCCAGCGCTTCGTCGACAGCTTCGCCCGCGTCTACACCCCGGTGGTGTTCCTCATCGCCCTGGCCACCGCGG
TGCTGCCGCCATTGCTGCTCGGCGGCGCCTGGCTGGACTGGATCTACCGGGCGCTGGTGCTGCTGGTGATCGCCTGTCCC
TGCGCCCTGGTGATCTCGACCCCGGTGACCATCGTCAGCGGCCTGTCCGCCGCGGCGCGCCTGGGCATCCTGATCAAGGG
CGGGGTGTTCCTCGAACTCGGCCGCAAGCTGTCCTGGGTCGCCCTCGACAAGACCGGCACCATCACCCACGGCAAGCCGC
AGCAGACCGATTACCTGCCGATCGCGGAAGATGAAGGGACGGATGCGCGCCTGCTCGCCGCCAGCCTGGCCGCGCGTTCC
GACCACCCGGTATCGCGCGCCGTGGCCAACGCAGCGGAAGAGGACGGCCTCGCCCTGGGCGTGGTCGAGGACCTCACCGC
GCTGCCGGGACGCGGTGTCAGCGGACGCATCGATGGCGTGCTCTACCACCTCGGCAACCATCGCCTGGTGGAAGAACTGG
GGCTCTGCTCGCCAGCCCTGGAAGAGCGCCTGGACGCCCTGGAGCGCCAGGGCAAGACCGTCATCGCGCTGTGCGACCCG
CAGCGGGTGCGGGCCCTGTTCGCGGTCGCCGACGGGGTCAAGGACAGCAGCCGCGAGGCGATCCGCGAGCTGCACCAACT
GGATGTGAAGACCCTGATGCTGACCGGCGACAACCCGCACACCGCCGCCGCCATCGCCAGCCAGGTGGGTATCGACGCGG
CGCGCGGCAACCTGCTGCCGGAAGACAAGCTGCGCGAAGTGGAAGCGCGCCAGGCCGACGGTTCGCGGGTCGGCATGGTC
GGCGACGGGATCAACGACGCACCCGCGCTGGCGCGCGCCGACATCGGCTTCGCCATGGGCGCGGCGGGCACCGACACCGC
CATCGAGACCGCCGGCGTGGCGCTGATGGACGACGACCTGCGCAAGCTGCCGCAGTTCATCCGCCTGTCGCGTACCACCC
ACGCGATCCTGATCCAGAACATCAGCCTCGCCCTGGGTATCAAGGCGGTGTTCCTCGCCCTCACCCTGGCCGGCGAAGGC
ACCCTGTGGATGGCGGTGTTCGCCGACATGGGCGCTAGCCTGCTGGTGGTGTTCAACGGCCTGCGCCTGTTGCGCAAGCG
TTTCTGA

Protein sequence :
MSHEHADTCCHGHGHGHGHGHGHGHDHGHRHAPRPALAAIGTLAGNAELRWSDLRIEAMDCPTEERLIRDALGRQPAVES
LEFNLMQRLLRVQHRFDSVEPLQKLIAGLGMQAVPLDAGEGLDGPSEAPTKPWWPLALAGVMALASEVVHWFGLAPDWVV
AGLALLAILGAGLPTYRKGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVSVLFAIAELIEAKSLDRARNAIRGLLQ
LAPEQATVLVGGEWKELPAKQVELEATVRVKPGERIALDGEVISGRSSVNQAPITGESLPVEKEVGEPLFAGSINGEGAL
EYRVTRRADDSTLARIIHAVEEAQGSRAPTQRFVDSFARVYTPVVFLIALATAVLPPLLLGGAWLDWIYRALVLLVIACP
CALVISTPVTIVSGLSAAARLGILIKGGVFLELGRKLSWVALDKTGTITHGKPQQTDYLPIAEDEGTDARLLAASLAARS
DHPVSRAVANAAEEDGLALGVVEDLTALPGRGVSGRIDGVLYHLGNHRLVEELGLCSPALEERLDALERQGKTVIALCDP
QRVRALFAVADGVKDSSREAIRELHQLDVKTLMLTGDNPHTAAAIASQVGIDAARGNLLPEDKLREVEARQADGSRVGMV
GDGINDAPALARADIGFAMGAAGTDTAIETAGVALMDDDLRKLPQFIRLSRTTHAILIQNISLALGIKAVFLALTLAGEG
TLWMAVFADMGASLLVVFNGLRLLRKRF

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 66
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 2e-115 47
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 2e-115 47
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 1e-114 46

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA2 YP_001346832.1 cadmium-translocating P-type ATPase BAC0298 Protein 0.0 68
cadA2 YP_001346832.1 cadmium-translocating P-type ATPase BAC0461 Protein 1e-83 42
cadA2 YP_001346832.1 cadmium-translocating P-type ATPase BAC0100 Protein 9e-61 41