Gene Information

Name : lysS (Asuc_1515)
Accession : YP_001344808.1
Strain : Actinobacillus succinogenes 130Z
Genome accession: NC_009655
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 1677731 - 1679242 bp
Length : 1512 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCAGAACAACAAAATACAGAATTAGACTTTCACGGTGAAATGGCGGTGCGTCGCGAAAAATTAGCGGCGTTACGTGC
AAAAGGCAACGCCTTTCCGAACACTTTCCGCCGTGATGCGTTAGCGCAGGATTTGCATCATCAATACGACGAAACGGACG
GCGAACAATTAAAAGAAAAGAACCCTCAGGTTGCTGTCGCCGGCCGTATTATGACCCGTCGGGCCATGGGTAAAGCTACG
TTTATCACCATTCAGGATATGAGCGGCAAAATTCAGCTTTATGTAGCATGCGACAACCTGCCGGAAGGCGTTTACGCGGA
AGATGTAAAAAGCTGGGATTTGGGCGACATTGTCGGCATCAAAGGCACGTTATTCAAAACAAAAACCAACGAATTAACCG
TAAAAGCCCACGAAGTACAATTACTCACCAAAGCGCTTCGTCCGCTGCCGGACAAATTCCACGGCTTAAGCGACCAAGAA
ACCCGTTATCGTCAACGTTATCTAGACTTAATTTCTAACGAAGAATCCCGCCGCACTTTTGTCATTCGTTCAAAAGTGAT
CGCCGGTATTCGCGAATACTTTATCGGCAAAGGGTTCATCGAAGTGGAAACGCCAATGCTGCAAGTGATCCCGGGCGGCG
CGGCGGCACGTCCGTTCGTGACGCACCACAACGCTTTAGACATTGATATGTATTTACGTATTGCGCCGGAACTTTACTTA
AAACGTTTAGTCGTCGGCGGTTTCGAACGCGTATTCGAACTCAACCGCAATTTCCGTAACGAAGGGGTTTCCGTACGCCA
CAATCCGGAATTTACTATGATCGAATACTATCAAGCCTATGCGGATTACCACGATTTAATGGATAACACGGAAGAATTGT
TACGCAAACTGGCGTTGGATATTTTAGGCACCACCATCGTGCCTTACGGTGAATATGAATTCGATTTCGGCAAACCGTTC
GAACGCATCACTATGCACGATGCGGTGCTTAAATACGGCGCAGAAAAAGGTATTGTCAAAGAAGATTTATATGATTTAGA
ACGCGCCAAAGCCGCGGCGACAAAACTCGGCATTGAAATTCAAAAATCCTGGGGATTAGGTTCCGTTGTGAACGCGATTT
TCGAAGAAGTGGCGGAACATCATTTAATTCAGCCGACCTTCTTAACGGCGCATCCTGCGGAAATTTCACCGTTGGCACGC
CGTAACGACGAAAATCCGGAGGTGACCGATCGTTTCGAATTATTTATCGGCGGTCGTGAAATCGGTAACGGTTTTTCGGA
ATTAAACGATGCGGAAGACCAGGCTGAACGTTTCGACGCCCAAGTGGCGGCAAAAGACGCGGGCGATGATGAAGCTATGT
TCAAAGACGACGATTTCGTCACCGCACTGGAACACGGCCTACCGCCGACAGCCGGCGAAGGTTTGGGTATCGACCGTTTG
GCAATGCTGTTCGCCAATGCGCCGTCAATCCGTGATGTGATTTTATTCCCGGCGATGAAACACAAAGCGTAA

Protein sequence :
MSEQQNTELDFHGEMAVRREKLAALRAKGNAFPNTFRRDALAQDLHHQYDETDGEQLKEKNPQVAVAGRIMTRRAMGKAT
FITIQDMSGKIQLYVACDNLPEGVYAEDVKSWDLGDIVGIKGTLFKTKTNELTVKAHEVQLLTKALRPLPDKFHGLSDQE
TRYRQRYLDLISNEESRRTFVIRSKVIAGIREYFIGKGFIEVETPMLQVIPGGAAARPFVTHHNALDIDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLALDILGTTIVPYGEYEFDFGKPF
ERITMHDAVLKYGAEKGIVKEDLYDLERAKAAATKLGIEIQKSWGLGSVVNAIFEEVAEHHLIQPTFLTAHPAEISPLAR
RNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDDDFVTALEHGLPPTAGEGLGIDRL
AMLFANAPSIRDVILFPAMKHKA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-153 63
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-89 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001344808.1 lysyl-tRNA synthetase VFG1668 Protein 9e-154 63