Gene Information

Name : entE (KPN_00612)
Accession : YP_001334292.1
Strain : Klebsiella pneumoniae subsp. pneumoniae MGH 78578
Genome accession: NC_009648
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 693667 - 695274 bp
Length : 1608 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGATTGCATTTACCCGTTGGCCGGAAGAGTTTGCGGCCCGCTATCGTCAAAAAGGCTACTGGCAGGATCTGCCGTTAAC
CAATCTGATCACCCGTCACGCGGACAATGACGCCGTGGCGATTATCGATGGCGAGCGGCAGATTAGCTACCGCCAGTTTA
ACCAGCTGGTGGACAACCTCGCCTGCTCGCTGCAGCGCCAGGGACTGAAGCGCGGGGAGACCGCGCTGGTGCAGCTGGGC
AATGTCGCCGAGTTTTACATGACCTTCTTTGCGCTGCTGCGCATCGGCGTCGCGCCAGTGAACGCTTTGTTCAACCACCA
GCGCAGCGAACTTAACGCCTACGCCGCGCAGATTAAACCGGCGCTGCTGATTGCCGATCGCGAGCACGCTCTGTTTGCTG
ACGATAGCTTTCTCCACGCGTTTATCGCGGAACATCCTTCCCTGCGCGTCGCACTGTTGCGTAACGACGGCGGCGAGCGC
GATCTGGCGACGGAGATTAACCGCCCGGCGGATAACTTTATCGCCAATCCAACCCCTGCCGACGAAGTGGCCTTTTTCCA
GCTCTCCGGCGGCAGCACCGGGACGCCGAAGCTTATCCCTCGCACCCATAACGATTACGACTACAGCATCCGCCGCAGCA
ACGAGATCTGCGGGATTAACGCTGAAACGCGCTACCTCAACGCGCTGCCGGCGGCGCATAACTATGCGATGAGTTCCCCG
GGTTCGCTCGGCGTCTTCCTCGCCGGCGGGCGGGTGATCCTCGCCGCTGACCCGAGCGCCACGCTCTGTTTCCCGCTGAT
CGAAAAACATCAGATTAACGTCGCGTCGCTGGTGCCGCCGGCGGTCAGCCTGTGGCTGCAGGCGATTCACGAATGGGGCA
GCAACGCCCAGCTGCAATCGCTGCAGCTGCTGCAGGTCGGCGGCGCGCGCCTGTCAGCGACCCTCGCCGCGCGCATTCCG
GCGGAAATCGGCTGCCAGCTGCAGCAGGTGTTCGGCATGGCGGAGGGGCTGGTGAATTACACCCGCCTTAACGATAGCCC
GGAGCGGATCATCAATACCCAGGGCTGTCCGATGTGCCCGGACGATGAAGTGTGGGTAGCGGATGCCGACGGCAACCCGC
TGCCGCGCGGCGAAGTGGGCCGCCTGATGACCCGCGGGCCGTACACTTTCCGCGGCTACTACAACAGCCCGCAGCATAAC
GCGGAAGCCTTTGACGCCGAGGGGTTTTATTGCTCCGGCGATCTGATTTCGATCGACGAAGATGGGTATATCACCGTCCA
GGGCCGGGAAAAAGATCAGATCAACCGCGGCGGCGAGAAGATCGCCGCTGAAGAGATCGAAAACCTGCTGCTGCGCCATG
AGGCGGTGATCCACGCCGCGCTGGTCAGCATCGAAGACAATCTGCTGGGCGAGAAAAGCTGCGCTTATCTGGTGGTGACC
TCGCCGCTGCGCGCCGTCGCCGTGCGCCGCTTCCTGCGCGAGCAGGGGGTGGCGGAATTTAAACTGCCGGACCGCGTGGA
GTGCGTTGCCGCGCTGCCGCTGACGCCGGTGGGCAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCCGAAGGCAAGC
TGGGCTGA

Protein sequence : 
MIAFTRWPEEFAARYRQKGYWQDLPLTNLITRHADNDAVAIIDGERQISYRQFNQLVDNLACSLQRQGLKRGETALVQLG
NVAEFYMTFFALLRIGVAPVNALFNHQRSELNAYAAQIKPALLIADREHALFADDSFLHAFIAEHPSLRVALLRNDGGER
DLATEINRPADNFIANPTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGINAETRYLNALPAAHNYAMSSP
GSLGVFLAGGRVILAADPSATLCFPLIEKHQINVASLVPPAVSLWLQAIHEWGSNAQLQSLQLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLNDSPERIINTQGCPMCPDDEVWVADADGNPLPRGEVGRLMTRGPYTFRGYYNSPQHN
AEAFDAEGFYCSGDLISIDEDGYITVQGREKDQINRGGEKIAAEEIENLLLRHEAVIHAALVSIEDNLLGEKSCAYLVVT
SPLRAVAVRRFLREQGVAEFKLPDRVECVAALPLTPVGKVDKKQLRQWLAEGKLG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 3e-86 41
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-86 41
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 5e-86 41
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-86 41
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 4e-86 41
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-86 41
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-86 41
ybtE CAA21394.1 - Virulence HPI Protein 4e-86 41
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 5e-86 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_001334292.1 enterobactin synthase subunit E VFG0932 Protein 0.0 81
entE YP_001334292.1 enterobactin synthase subunit E VFG0168 Protein 8e-105 45