Gene Information

Name : lysS (Smed_2800)
Accession : YP_001328465.1
Strain : Sinorhizobium medicae WSM419
Genome accession: NC_009636
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 2922188 - 2923684 bp
Length : 1497 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACCGATAAGACAGAAAACTCCGGCCTTTCCTCCGATGCGACGGAAGTACGCGCCCAGAAGCTGAAGCTCCTGCGCGA
GCAGATCGGCGATGTCTATCCCGCGCATTTCCATCGGACGATGACCAATGCGGAATTGGCCGAGAAGTACCAGGACCTCG
CGCCGGACACGGAGACGCAGGACCTCGTCACCGTTGCCGGACGAGTCTATTCCTCGCGCAATTCCGGCATGTTCATGGAC
ATCCATGACGCTTCCGGCAAGATCCAGGTCTTCTCCCACAAGGACACGACGCCTGAGGAGGCGCGTGGCCTCCTGCCGAT
GATCGACCTCGGAGATGTCATCGGCGTCACCGGGCTCGTCCGCCGCACGAAGCGCGGCGAACTGACGATCAACGCGCAGC
AGATCACCATGCTGACCAAGTCGCTCCTGCCGATGCCGGAAAAGTATCACGGCCTTGCAGACATCGAGACGCGCTATCGC
AAGCGATATCTGGACATCATGGTCAATGAGGAATCGAAGCTGCGCTTCCAGCAAAGAAGTCGCATCGTCTCCAGCCTGCG
CCGCTTCCTCGAGGACGAAGGCTTCATGGAAGTCGAGACGCCGATGCTGCAGCCGATCTACGGTGGCGCGACGGCCGAGC
CCTTCAAGACCCATCACAATACGCTGAAGCTCGATATGTATCTGCGCATCGCGCCCGAGCTTTATCTCAAGCGCGTGCTG
GTCTCCGGTCTCACCGACAAAGTCTTCGAAATCAACCGCAACTTCCGCAACGAAGGCGTCTCCACCCGGCACAATCCGGA
ATTCACCATGATGGAGTGCTATTGGGCCTATGCCGACTATGAAGACGTAATGGGCCTCGTCGAACGTATGTTCGAGACCC
TTGCTCTGGCGGTCCACGGCACGACCGAATTCGAGTTCGGCGACAAGCAGCTCTCCTTCAAGGGGCCGTTCCCGCGCGTC
TCCATGCCCGCCGCTGTCAAGGACGCGACCGGCATTGATTTCCTAGCGATCAAAAGCGACGAGGAGGCCCGACAGGCGGC
TCGCGATGCCGGTGTCGAGATCGAGAAGGACGCGACATGGGGCGAGGTGCTCGCCTTCCTCTTCGAGGAAAAGGTCGAAG
CGACGCTGATCCAGCCTGCCCACGTCATCCATTTCCCGAAGGACATCTCGCCCTTCGCCAAAGAGGTGCCGGGCGAGCCG
AGGCTCGTCGAGCGGTTCGAGACCTATTGCAACGGATGGGAGATCGGCAACGCCTTTTCCGAGCTCAACGACCCGGTCGA
GCAACGCGCTCGTATGGTCGAACAAATGGAACAGGCGCACGCACGCGGCGAGAAGGAAAAGACGCTCGACGAGGACTTCC
TCGATGCCATGGACCAGGGCATGCCGCCCGCAGGTGGTTTGGGGATCGGTGTCGACCGGCTGATCATGCTGCTCACCAAC
TCGCCGTCGATCCGCGACATCATTCTCTTCCCGGCCCGACGCCATAAGTCCGATTGA

Protein sequence :
MTDKTENSGLSSDATEVRAQKLKLLREQIGDVYPAHFHRTMTNAELAEKYQDLAPDTETQDLVTVAGRVYSSRNSGMFMD
IHDASGKIQVFSHKDTTPEEARGLLPMIDLGDVIGVTGLVRRTKRGELTINAQQITMLTKSLLPMPEKYHGLADIETRYR
KRYLDIMVNEESKLRFQQRSRIVSSLRRFLEDEGFMEVETPMLQPIYGGATAEPFKTHHNTLKLDMYLRIAPELYLKRVL
VSGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDVMGLVERMFETLALAVHGTTEFEFGDKQLSFKGPFPRV
SMPAAVKDATGIDFLAIKSDEEARQAARDAGVEIEKDATWGEVLAFLFEEKVEATLIQPAHVIHFPKDISPFAKEVPGEP
RLVERFETYCNGWEIGNAFSELNDPVEQRARMVEQMEQAHARGEKEKTLDEDFLDAMDQGMPPAGGLGIGVDRLIMLLTN
SPSIRDIILFPARRHKSD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 9e-104 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001328465.1 lysyl-tRNA synthetase VFG1668 Protein 5e-104 47