Gene Information

Name : Amet_4591 (Amet_4591)
Accession : YP_001322321.1
Strain : Alkaliphilus metalliredigens QYMF
Genome accession: NC_009633
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 4704634 - 4705734 bp
Length : 1101 bp
Strand : -
Note : TIGRFAM: D-alanine--D-alanine ligase; PFAM: ATP-dependent carboxylate-amine ligase domain protein, ATP-grasp; protein of unknown function DUF201; D-alanine--D-alanine ligase domain protein; RimK domain protein ATP-grasp; KEGG: cth:Cthe_1938 D-alanine--D-a

DNA sequence : 
GTGAAGAAGATCAATGTAATGGTGATTTTTGGAGGACAATCCGGAGAACATGAAGTTTCACTGATGTCAGCAGCTTCTGT
CATGGGCGCCATCAATAAGGAAAAATATCATATTATTCCCGTGGGAATTGCAAAGAATGGTGATTGGAAGCTTTTTAATG
GATCTGTTGAGGAAATTGAATCTGGAGACTGGGAACAAAGTATAAATGAACTGACAGAGAAGGGCGAGGACAATAAAAAG
TTCTCTCTTTTACCATTAACCGATGGGAAGCAAGGAATTGTCCTGCAAAACGGGGAAGTGAAAAAGATAGATGTTATTTT
CCCTGTACTCCATGGCCCCTATGGGGAGGATGGACGAATTCAAGGATTGTTTGAAATGATCGACATTCCCTACGTAGGAT
GTGGTGTGATGGCCTCAGCCGTCGCCATGGATAAGGTGATAACCAAAAAAGTATTAGAAGCAGAAAAGATTGCGCAAACT
CCCTATCAAGTCATCTATGGGAGACGATTTCAATTGGATCCATTAAAGTATCTTAACGACTTGGAAGCGACCTTTGAATA
CCCTGTATTTGTGAAGCCTGCAAACATGGGCTCTAGTGTAGGGATTTCAAAGGCAAAAAACAGGGAAGCCTTGAAGATAG
CCATAAAAGAAGCCGGAAAGTATGATAATAAAATTGTGATCGAAGCCTTTGTGGAGGGTCGGGAGATTGAGTGTGCAGTC
TTAGGAAACGAAGCACCTCGGGTGGCACTGCCTGCAGAAATTATTCCATCCCATGAGTTTTATGATTATCATGATAAATA
CTTTGATGGAACAAGTCGGTATGTCATTCCCCCAGATCTATCAGAAGAGTTGATCCAAGAGATACAACACTTAGCAATTC
AAACCTATGAGCTACTAGACTGTCGAGGACTATCTAGAATAGACTTCTTTGTAGAAAATGAAACAAATCGAGTGTTGGTC
AATGAGGTGAATACAATGCCTGGATTTACAAAAATCAGCATGTACCCAAAGATGTGGGAGGCTACTGGATTGCCATATGA
TAAACTAATCGATGAATTGATTCAACTGGCTATGGAAGAATACCAATTAAAATCGCGATAA

Protein sequence : 
MKKINVMVIFGGQSGEHEVSLMSAASVMGAINKEKYHIIPVGIAKNGDWKLFNGSVEEIESGDWEQSINELTEKGEDNKK
FSLLPLTDGKQGIVLQNGEVKKIDVIFPVLHGPYGEDGRIQGLFEMIDIPYVGCGVMASAVAMDKVITKKVLEAEKIAQT
PYQVIYGRRFQLDPLKYLNDLEATFEYPVFVKPANMGSSVGISKAKNREALKIAIKEAGKYDNKIVIEAFVEGREIECAV
LGNEAPRVALPAEIIPSHEFYDYHDKYFDGTSRYVIPPDLSEELIQEIQHLAIQTYELLDCRGLSRIDFFVENETNRVLV
NEVNTMPGFTKISMYPKMWEATGLPYDKLIDELIQLAMEEYQLKSR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 2e-53 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Amet_4591 YP_001322321.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 7e-54 41
Amet_4591 YP_001322321.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 7e-54 41
Amet_4591 YP_001322321.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 7e-54 41
Amet_4591 YP_001322321.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 4e-53 41
Amet_4591 YP_001322321.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 3e-53 41