Gene Information

Name : Smed_6486 (Smed_6486)
Accession : YP_001314974.1
Strain :
Genome accession: NC_009622
Putative virulence/resistance : Unknown
Product : transposase IS66
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG3436
EC number : -
Position : 178952 - 180520 bp
Length : 1569 bp
Strand : -
Note : PFAM: transposase IS66; KEGG: ret:RHE_PA00098 probable insertion sequence transposase protein, IS66 family

DNA sequence :
GTGGCATGGAAACAACGCCGCTGCACACTCAGGACGAGCTATCTGCTTTGCGCGCGCTCGTCGCTGAACAGGCGGCGAAA
CTCAGCGCAAGAAGCCGAAGTCAGCAAGCGCGACTCCATTATCGGCCTTCTGCGCGCGCAGTTGGAACTGCTGCGCCATC
GACAACATGGCCCTTCTTCGGAAAAGATAGACCGCAAAATCGAGCAATTCGAGCTGATGCTGGAGGAGATCGAGGCTTCT
CGCGCTGAGGCCGATGCGCGCTCTGCGAACTCCCCCTTGCCGGAGTTAGACGACGAACCGGACAAGCCGAAGCGCAGACC
GCTGCCGGATGGCCTTCCAACTGAGGAGCTGGTCTACGCGGCACCCTGCAATTGTCCGACTTGTGGTGGCACATCTTTCC
TGAAGGCGGCTGACAAGGTGGTCCAGGTGATGGAGCACGTACCGGCGTCCGTAAAGATTGTCCGCCATATCGAAAAGCGC
ATGGTCTGCAAGGACTGCGATACGACGGTGTCTGGCGAAATGCCGACCTTGCCGATCGGGCGAGGCAAGCCCGGTCCGGG
GTTGCTCGCGCATATCATGGTCGCCAAGTTCGACGATCACATCCCGCTCTACCGTCTCTCCGAGATGTACGACCGCCTGG
GGATAGACATCTCCCGGTCTGTCATGGCGGACTGGGTTGGCCGTGTGTCCGTTCTGCTTTCGCCCCTCATCTTGCTGATC
AGAGCCCATATCGCCGCGGTCGACCGAATACACACGGACGATACCCCGGTTGATGTTCTCGACCCTGGACGGGGCAAAAC
GAAAACCGGCAGAGTCTGGGTCTATGTCTTCGATGGCAGCGGGTATCAAGATCCCACTCCCAGAGCTGTCGCCTATTACT
ATAGCCCCGACCGCAAGGGCGCGCATCCGGCAGACCACCTCGCTGACTTCAGCGGCGTGATGCATGCCGATGGCTACGGT
GGCTATAAGAAGCTTTACGGCAACCAGATCGTTGAGGCCGCGTGCATGGCGCACGTGCGCCGCAAGTTCCATGACGTAAT
CAAGCTGAAGCCATCACCGATAGCCGAGGAGGCGCTGTCTCGCATCGGCGCGCTCTACGATATCGAGGATCGGATCCGCG
GCATGCCGGCCGCCGAGCGCCAAATGCTGCGACAAGAGCACGCTGGTCCCATTCTGAGAGACCTGAAGTTCTGGATCGAG
GAAACGCTTCCGACTTTGCCGCAGAAACAGAAGTTGGCCGAGGCGATGCGATATGCCCTCTCGCGGTGGGCGGCATTGAG
CGTTTACATCGACGATGGGCGCGTCGAGATCGACAACAATATAGCTGAACGAGCCATGCGCCCGCTTGGAATTGGTCGAA
AGAACTGGCTCTTCGCCGGCTCCGACAAGGGCGGCGAGCGCATCGCGGATATCCTGACTATCATCGAGACGGCCAAACAA
CATGGCCTCAATCCGGAGGTCTATCTGACAGACGTCCTAACCCGGATCCAAGATCATCCCACGGATCGCCTTGAGGAACT
GCTGCCGTGGCAGTGGGCGCCGGCAAAAGACCTGCACGAGGCTGCCTGA

Protein sequence :
MAWKQRRCTLRTSYLLCARSSLNRRRNSAQEAEVSKRDSIIGLLRAQLELLRHRQHGPSSEKIDRKIEQFELMLEEIEAS
RAEADARSANSPLPELDDEPDKPKRRPLPDGLPTEELVYAAPCNCPTCGGTSFLKAADKVVQVMEHVPASVKIVRHIEKR
MVCKDCDTTVSGEMPTLPIGRGKPGPGLLAHIMVAKFDDHIPLYRLSEMYDRLGIDISRSVMADWVGRVSVLLSPLILLI
RAHIAAVDRIHTDDTPVDVLDPGRGKTKTGRVWVYVFDGSGYQDPTPRAVAYYYSPDRKGAHPADHLADFSGVMHADGYG
GYKKLYGNQIVEAACMAHVRRKFHDVIKLKPSPIAEEALSRIGALYDIEDRIRGMPAAERQMLRQEHAGPILRDLKFWIE
ETLPTLPQKQKLAEAMRYALSRWAALSVYIDDGRVEIDNNIAERAMRPLGIGRKNWLFAGSDKGGERIADILTIIETAKQ
HGLNPEVYLTDVLTRIQDHPTDRLEELLPWQWAPAKDLHEAA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
c3563 NP_755438.1 hypothetical protein Not tested PAI I CFT073 Protein 3e-80 49
BCAM0248 YP_002232880.1 putative transposase Not tested BcenGI11 Protein 3e-97 48
st57 CAC81895.1 ST57 protein Not tested LEE II Protein 1e-88 48
aec53 AAW51736.1 Aec53 Not tested AGI-3 Protein 2e-82 46
unnamed CAC39285.1 hypothetical protein Not tested LPA Protein 3e-93 45
ECs4547 NP_312574.1 hypothetical protein Not tested LEE Protein 2e-93 45
tnp AEA34686.1 transposase Not tested Not named Protein 2e-93 45
unnamed ACU09439.1 IS66 family element transposase Not tested LEE Protein 1e-93 45
Z4337 NP_289562.1 hypothetical protein Not tested OI-122 Protein 5e-93 45
unnamed AAC31494.1 L0015 Not tested LEE Protein 3e-93 45
Z5098 NP_290249.1 prophage-associated protein Not tested LEE Protein 5e-93 45
unnamed CAI43806.1 hypothetical protein Not tested LEE Protein 1e-92 45
unnamed AAL57570.1 unknown Not tested LEE Protein 4e-93 45
Z1131 NP_286666.1 hypothetical protein Not tested TAI Protein 3e-92 44
Z1570 NP_287074.1 hypothetical protein Not tested TAI Protein 3e-92 44
Z4340 NP_289564.1 hypothetical protein Not tested OI-122 Protein 7e-85 44
Z1161 NP_286696.1 hypothetical protein Not tested TAI Protein 5e-86 42
Z1600 NP_287104.1 hypothetical protein Not tested TAI Protein 5e-86 42
unnamed AAL08460.1 unknown Not tested SRL Protein 9e-66 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Smed_6486 YP_001314974.1 transposase IS66 VFG1700 Protein 1e-80 49
Smed_6486 YP_001314974.1 transposase IS66 VFG0793 Protein 2e-93 45
Smed_6486 YP_001314974.1 transposase IS66 VFG1051 Protein 5e-66 42
Smed_6486 YP_001314974.1 transposase IS66 VFG1736 Protein 8e-69 41