Gene Information

Name : lysS (CGSHiGG_09780)
Accession : YP_001293113.1
Strain : Haemophilus influenzae PittGG
Genome accession: NC_009567
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 1805876 - 1807384 bp
Length : 1509 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCAGAACAAGAAGTTAAAGAATTAGATCTCAATGGCGAAATGCTCGTTCGTCGTGAAAAATTAGCCGCATTGCGAGC
AAAAGGTAATGCCTTTCCAAACAAGTTTCGTCGTGATGTGCTCGCACAAGATTTACACAATCAATATGATTCAGAAGACG
GGGAAATCTTAAAAGAGAAAGGCGTTGAAGTACAAGTTGCTGGTCGCATTATGACTCGCCGTGCAATGGGTAAAGCAACG
TTTATCACTATTCAAGATATGAGCGGTAAAATCCAGCTTTACGTTGCACGCGATAATTTGCCTGAAGGTGTTTATAAAGA
TGAAGTTGGTACTTGGGATTTAGGCGACATCATTGGCGTTAAAGGCACTTTATTTAAAACAAAAACTGATGAACTCACTG
TAAAAACCACTGAAGTTCAACTTTTAACCAAAGCACTTCGCCCATTACCAGATAAATTCCACGGTTTAACCGACCAAGAA
GTTCGTTATCGCCAACGTTATTTAGATTTAATTTCTAACGAAGAATCTCGCCGCACTTTTATTATTCGTTCTAAAGTTGT
CGCGGGTATTCGTGAATATTTCATTTCTAAAGGCTTTATGGAAGTGGAAACGCCAATGTTACAAGTTATTCCAGGCGGTG
CATCTGCGCGTCCTTTCGTAACTCATCACAATGCATTAGATGTGGATATGTATTTACGTATCGCACCTGAACTTTATTTA
AAACGCTTAGTAGTGGGTGGCTTTGAACGAGTATTTGAATTAAACCGTAACTTCCGTAATGAAGGGGTTTCTGTTCGTCA
TAATCCAGAATTTACTATGCTTGAATACTATCAAGCCTATGCAGATTACCACGATTTAATGGATAACACCGAAGAACTGC
TTCGTAAATTAGCGATTGATATTCTTGGTACTACTATTGTGAAATACGGCGAATACGAATTTGATTTTGGTAAACCATTC
GAACGTATTACGTTACACGATGCAACGGTTAAATATGGTGCAGACAAAGGTATCGTAAAAGAAGATTTATATGACTTTGA
TCGTGCAAAAGCCACGGCAGAACGCTTAGGTATTGAAGTACAAAAATCTTGGGGTTTAGGCAGCATTGTCAATGCAATTT
TTGAAGAAGTGGCTGAACATCACTTAATTCAACCAACCTTCTTAATGGCTCATCCTGCGGAAATTTCTCCACTTGCACGT
CGTAATGATGAAAATCCAGATGTAACAGATCGTTTTGAGCTCTTTATCGGTGGTCGTGAAATCGGTAATGGTTTCTCAGA
ATTAAATGACGCAGAAGATCAAAATGAACGTTTTGATGCACAAGTGGCTGCAAAAGAAGCTGGCGATGATGAAGCGATGT
TTAAAGATGAAGACTTTGTTGTTGCATTAGAGCACGGCTTACCACCAACAGCTGGGGAAGGTTTAGGTATCGACCGCCTA
GCGATGCTTTATGCAAATGCACCGTCAATTCGTGATGTGATCTTATTCCCTGCAATGCGTCAGAAATAA

Protein sequence :
MSEQEVKELDLNGEMLVRREKLAALRAKGNAFPNKFRRDVLAQDLHNQYDSEDGEILKEKGVEVQVAGRIMTRRAMGKAT
FITIQDMSGKIQLYVARDNLPEGVYKDEVGTWDLGDIIGVKGTLFKTKTDELTVKTTEVQLLTKALRPLPDKFHGLTDQE
VRYRQRYLDLISNEESRRTFIIRSKVVAGIREYFISKGFMEVETPMLQVIPGGASARPFVTHHNALDVDMYLRIAPELYL
KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMLEYYQAYADYHDLMDNTEELLRKLAIDILGTTIVKYGEYEFDFGKPF
ERITLHDATVKYGADKGIVKEDLYDFDRAKATAERLGIEVQKSWGLGSIVNAIFEEVAEHHLIQPTFLMAHPAEISPLAR
RNDENPDVTDRFELFIGGREIGNGFSELNDAEDQNERFDAQVAAKEAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRL
AMLYANAPSIRDVILFPAMRQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-151 63
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 1e-88 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001293113.1 lysyl-tRNA synthetase VFG1668 Protein 1e-151 63