Gene Information

Name : Pput_5012 (Pput_5012)
Accession : YP_001270316.1
Strain : Pseudomonas putida F1
Genome accession: NC_009512
Putative virulence/resistance : Resistance
Product : heavy metal translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 5634760 - 5637012 bp
Length : 2253 bp
Strand : -
Note : TIGRFAM: ATPase, P-type (transporting), HAD superfamily, subfamily IC; cadmium-translocating P-type ATPase; heavy metal translocating P-type ATPase; PFAM: Haloacid dehalogenase domain protein hydrolase; E1-E2 ATPase-associated domain protein

DNA sequence :
ATGAACCAGCCTGTCAGCCACGAACACAAGCATCCGCACGATCACGCTCATGGTGATGACGACCATGGCCATGCTGCCCA
CGGCCACAGCTGCTGCGGCGCCAAAGCCGCGCCGCCGCTGGTGCAACTGAGCGAAACGGCCAGTGCCCAGGCACAACTCA
GTCGTTTCCGCATCGATGCCATGGACTGCCCCACCGAGCAGAACCTGATTCAGGACAAACTGAGCAAGCTGGCCGGTATC
GAACAGCTGGAATTCAACCTGATCAACCGCGTGCTTGGCGTGCGCCACACCTTGGACGGCACCGCCGATATCGAGCGGGC
TATCGACAGCCTGGGCATGAAGGCCGAGCCGATTGCCGCCCAGGACGACGGCAGCGCCAGCGTGCCCCAACCGGCCAAGG
CCCGCTGGTGGCCACTGGCATTGTCGGGCGTCGCCGCGATCGCCGCCGAAATCGTGCACTTTGCTGCACTGGCCCCTGAG
TGGGTGGTAGCGGGACTGGCGCTTGCCGCGATCCTCGGTTGCGGCCTGGGTACCTACAAGAAGGGCTGGATTGCCCTGAA
AAACCGTAACCTCAACATCAATGCGCTGATGAGTATCGCCGTGACGGGGGCCGTATTGATCGGCCAGTGGCCGGAGGCGG
CGATGGTCATGGTGCTCTTCACCGTTGCCGAACTGATCGAAGCGCGCTCGCTGGACCGTGCGCGCAATGCCATCGGCGGG
TTGATGCAGCTGACCCCGGACGTGGCTACGGTGCAGCAGGCCGATGGCCAGTGGCGCGAGCTGGAAGTGCGTGAGGTGGC
CATCGGCGCACTGGTTCGGGTGCGCCCCGGTGAGCGCATCGGCCTGGACGGTGAAGTGACCAGCGGGCAATCCAGCGTCG
ATCAGGCGCCGATTACCGGCGAAAGCCTGCCTGTGGAGAAGGGGGTGGGCGACAAGCTGTTCGCTGGCACCATCAACCAG
GCGGGGGCGCTGGAGTTTCGCGTCACCGCCGCTGCCGGGCAGTCGACCCTGGCGCGGATCATCAAGGCTGTGGAGGAAGC
GCAGGGCGCGCGGGCGCCTACCCAACGCTTCGTCGACCGTTTCTCGCGTATCTACACCCCGGTGGTATTCGCCATTGCCC
TGGCCGTGGCGCTGTTGCCCCCGCTGTTCATGGCTGGCGCCTGGTTCGACTGGGTTTACCGCGCCTTGGTGCTGCTGGTG
GTGGCCTGCCCCTGCGCGCTGGTGATCTCGACCCCGGTGACCATCGTCAGTGGCCTGGCCGCCGCTGCGCGCAAAGGCAT
CCTGATCAAGGGTGGCGTGTACCTGGAGGGTGGCCGCCACCTGGATTTCCTGGCCCTGGACAAGACCGGCACCATTACCC
ATGGCAAGCCGGTGCAGACTGACGCCAAGGTGCTGGCGCCGCTGTTCGAAGACCGTGCGCAAGCCTTGGCGGCCAGCCTG
GGCGAGCGTTCGGACCACCCGGTGTCCCGCGCCATTGCCGAGTTCGGCAAGCAGCAAGGCCTGGCCTTGAGCGAGGTCAG
TGACTTCGCCGCCCTGGCCGGGCGTGGCGTGCGTGGTGTCATCGCCGGTGAGGTCTACCACTTGGGCAACCACCGCCTGG
TCGAGGAACTGGGGCTGTGTTCGCCAGCGCTGGAGGCCCAGCTTGATGCGCTGGAGCGCCAGGGCAAGACCGTGGTCCTG
CTGCTCGACCGATCCGGCCCTCTGGCACTGTTCGCCGTGGCCGACACGGTCAAGGACAGCAGCCGTCAGGCGATCGCCGA
ATTGCATCAACTGGGTATCAAGACTGTCATGCTGACCGGTGACAATCCGCATACTGCGCAGGCCATTGCCGCCGTGGTGG
GGATCGACCGCGCCGAGGGCAACCTGTTGCCGGCCGACAAGCTGAAAACCATTGAGGCGTTGTACGCCCAAGGCCATCGG
GTAGGCATGGTCGGTGACGGCATCAACGACGCCCCGGCGCTGGCCCGCGCCGAAATCGGTTTTGCCATGGCTGCTGCCGG
CACCGACACCGCCATCGAGACGGCTGATGTGGCGCTGATGGACGATGACTTGCGCAAAATCCCGGCCTTCGTCAGGCTGT
CGCGCCAAAGTGCGGCGATCCTCATGCAGAACATCGTTCTGGCGTTGGGCATCAAGGCGATATTTCTGGCGATCACCTTT
GCCGGCATGGCCACGATGTGGATGGCGGTGTTCGCTGATATGGGCGTGAGCTTGCTGGTGGTGTTCAACGGCTTGCGCCT
TTTGCGCAAATAG

Protein sequence :
MNQPVSHEHKHPHDHAHGDDDHGHAAHGHSCCGAKAAPPLVQLSETASAQAQLSRFRIDAMDCPTEQNLIQDKLSKLAGI
EQLEFNLINRVLGVRHTLDGTADIERAIDSLGMKAEPIAAQDDGSASVPQPAKARWWPLALSGVAAIAAEIVHFAALAPE
WVVAGLALAAILGCGLGTYKKGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVMVLFTVAELIEARSLDRARNAIGG
LMQLTPDVATVQQADGQWRELEVREVAIGALVRVRPGERIGLDGEVTSGQSSVDQAPITGESLPVEKGVGDKLFAGTINQ
AGALEFRVTAAAGQSTLARIIKAVEEAQGARAPTQRFVDRFSRIYTPVVFAIALAVALLPPLFMAGAWFDWVYRALVLLV
VACPCALVISTPVTIVSGLAAAARKGILIKGGVYLEGGRHLDFLALDKTGTITHGKPVQTDAKVLAPLFEDRAQALAASL
GERSDHPVSRAIAEFGKQQGLALSEVSDFAALAGRGVRGVIAGEVYHLGNHRLVEELGLCSPALEAQLDALERQGKTVVL
LLDRSGPLALFAVADTVKDSSRQAIAELHQLGIKTVMLTGDNPHTAQAIAAVVGIDRAEGNLLPADKLKTIEALYAQGHR
VGMVGDGINDAPALARAEIGFAMAAAGTDTAIETADVALMDDDLRKIPAFVRLSRQSAAILMQNIVLALGIKAIFLAITF
AGMATMWMAVFADMGVSLLVVFNGLRLLRK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 67
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 1e-110 48
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 1e-110 48
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 1e-108 47

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Pput_5012 YP_001270316.1 heavy metal translocating P-type ATPase BAC0298 Protein 0.0 67
Pput_5012 YP_001270316.1 heavy metal translocating P-type ATPase BAC0455 Protein 8e-80 43
Pput_5012 YP_001270316.1 heavy metal translocating P-type ATPase BAC0461 Protein 9e-79 43
Pput_5012 YP_001270316.1 heavy metal translocating P-type ATPase BAC0092 Protein 5e-74 41