Gene Information

Name : lysS (MAG_6210)
Accession : YP_001256760.1
Strain : Mycoplasma agalactiae PG2
Genome accession: NC_009497
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : -
Position : 735485 - 736954 bp
Length : 1470 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGGAAAAATATACTGAGCAAGAGCAAGTCAGACGTAATAAATTAAATTTTTATAATGAAAATAACATTGCTCCATTTAA
AAAAGCTTACGACTTAGGCAAGTTAGTTTTAAGTCAAGAAATAGTTAATAATTATGACTCTTTTTCTAGGGAAGAATTAG
AAGAAAAAAATATTAGTGTTAATGTAGCCGGCAGATTAATGACTGTAAGAGGGCCATTTTTAGTATTAAATGACTCTAAA
GGCTTAGTTCAAGTTTATTTCAATAAAAAAAGTGATGAAAATGTAGCTAAAATAGTAGGCACATTTGATATTGGTGATAT
TTTATGAGTGAGTGGTACTGTTATGAAAACTCACACTAATGCACTTACCTTAAAGTGCAATAAAATAGAACTTTTAACTA
AAGCACTTAAGCCACTACCTGAAAAATATCATGGGCTTGTTGATACTGAAGAGAGATACAGACGCAGATGAGTTGACTTA
ATTACTAATGAAGAGTCAAAAAATAAGTTCATTTTAAGAACTAAAATTATTAAATGAATTAAAGAATACTTTGATAATTT
AGATTATTTGGAAGTTGAAACACCTTTCTTACAAGACTATGTTTCAGGAGCTGCTGCTAAACCTTTTACAACTCATCACA
ATTCTTTAAATCAAGAGTTTGTTTTAAGAATTGCTACAGAAATTCCGCTTAAAAAATGTGTAGTTGGCGGACTTGAAAGA
GTTTATGAATTAGGCAGAATTTTTAGAAATGAAGGATATGACACAACTCATAATCCTGAATTTACTACAATTGAGTTTTA
TGAAGCTTATTCTAATGTTGAAGGCATGATGGACAGAACTGAAGAATTGTTTAAGGTTTTATGTCAAAAATTAGGTAAGT
CAGTTTTTGTTAACAATGGTACTGAAGTTGATTTATCAAAACCATTTAAGAGAATTAATATGGTGGATGCAGTTAATGAA
AAAACTGGTAAGGACTTTAAAAACATTAGTTTGGACGAGGCTATTTTAGAGGCCAAAAAGCACAATATTAAAATTGAAAA
ATTCTGAACAACAGGGCATATAATTAATGCTCTCTATGAAGAGTTAGTGGAAAGCTCACTTATTCAACCAACCTTTATTT
ATGGTCATCCTATTGAAGTTTCACCTCTAAGTGCTAAAGCTAGCGATCCTAGATTTACTGAAAGAGCCGAATTATTTATT
AATACTAAAGAATATGCAAACATGTACACTGAGTTAAGTGATCCAATAGACCAGTTAGAAAGGTTTAAAAGTCAAATTGA
TGAAAAGAAAGCTGGCAATGATGAAGCTAGCGATATTGACTGAGATTTTGTGGACGCACTAGAATATGGAATGCCACCAA
CAGGCGGTTGCGGCATCGGTATTGATAGATTAATTATGCTTTTAACAGAAACTAGTTCAATAAGGGACGTGTTATTATTC
CCAACACTTAAAAGAGCGAATAAAAAATAA

Protein sequence :
MEKYTEQEQVRRNKLNFYNENNIAPFKKAYDLGKLVLSQEIVNNYDSFSREELEEKNISVNVAGRLMTVRGPFLVLNDSK
GLVQVYFNKKSDENVAKIVGTFDIGDILWVSGTVMKTHTNALTLKCNKIELLTKALKPLPEKYHGLVDTEERYRRRWVDL
ITNEESKNKFILRTKIIKWIKEYFDNLDYLEVETPFLQDYVSGAAAKPFTTHHNSLNQEFVLRIATEIPLKKCVVGGLER
VYELGRIFRNEGYDTTHNPEFTTIEFYEAYSNVEGMMDRTEELFKVLCQKLGKSVFVNNGTEVDLSKPFKRINMVDAVNE
KTGKDFKNISLDEAILEAKKHNIKIEKFWTTGHIINALYEELVESSLIQPTFIYGHPIEVSPLSAKASDPRFTERAELFI
NTKEYANMYTELSDPIDQLERFKSQIDEKKAGNDEASDIDWDFVDALEYGMPPTGGCGIGIDRLIMLLTETSSIRDVLLF
PTLKRANKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-91 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001256760.1 lysyl-tRNA synthetase VFG1668 Protein 7e-92 42