Gene Information

Name : CBO3570 (CBO3570)
Accession : YP_001256050.1
Strain : Clostridium botulinum ATCC 3502
Genome accession: NC_009495
Putative virulence/resistance : Unknown
Product : rRNA large subunit methyltransferase
Function : -
COG functional category : S : Function unknown
COG ID : COG1576
EC number : -
Position : 3814586 - 3815065 bp
Length : 480 bp
Strand : +
Note : SPOUT methyltransferase; crystal structure shows homodimer; in Escherichia coli this protein methylates pseudouridine at position 1915 of the 23S ribosomal RNA

DNA sequence :
ATGAATATATCTATAATTTCAGTAGGGAAAATAAAAGAAAAGTTTTTAAAGGCAGCAATAGACGAGTACTCTAAAAGATT
AAGTAAATACTGTAAACTAAATATAATAGAAGTGGCCGATGAAAAAACTCCAGATAATGCCTCTCTTAAAGAAGAAAATA
TAATAAAAGAAAAAGAAGGTAATTTAATATTAAAACATATAAAAGATAATAGCTTTGTTATAGCTTTAGATTTAAAAGGT
AAATCTATAACCTCAGAAGAATTCTCGGACCTTATAGAAAATTGCAGATTAACAGGTAATAGCACTATTGCCTTTGTTAT
CGGTGGATCCCTAGGATTATCTCAGCAGGTTTTATCCAGAGCTAATTATAAGCTATCTTTCTCAAAAATGACCTTCCCAC
ATCAGCTCTTTAGAGTAATGCTTCTAGAACAAGTTTATAGGGCTTTTAGAATATTATGCAGAGAACCGTACCACAAATAA

Protein sequence :
MNISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEGNLILKHIKDNSFVIALDLKG
KSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSRANYKLSFSKMTFPHQLFRVMLLEQVYRAFRILCREPYHK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed BAC53844.1 open reading frame X Not tested SRImec-III and SCCmec-III region Protein 6e-37 56
SAV0024 NP_370548.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 9e-37 56
unnamed BAC57492.1 hypothetical protein Not tested Type-IIIinv SCCmec Protein 6e-37 56
SE0023 NP_763578.1 rRNA large subunit methyltransferase Not tested SCCpbp4 Protein 6e-37 56
unnamed BAB72121.1 hypothetical protein Not tested Type-IVa SCCmec Protein 6e-37 56
SERP2529 YP_190070.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 6e-37 56
unnamed BAB72138.1 open reading frame X Not tested Type-IVb SCCmec Protein 6e-37 56
orfX YP_251938.1 rRNA large subunit methyltransferase Not tested SCCmec Protein 3e-37 56
unnamed BAC67575.1 open reading frame X Not tested Type-IVc SCCmec Protein 6e-37 56
SSP0026 YP_300116.1 rRNA large subunit methyltransferase Not tested SCC15305RM Protein 1e-37 56
unnamed BAA82243.1 orfX Not tested Type-II SCCmec Protein 6e-37 56
orfX AAL26648.1 unknown Not tested SCCcap1 Protein 5e-36 56
unnamed BAD24821.1 conserved hypothetical protein orfX Not tested Type-V SCCmec Protein 6e-37 56
orfX BAA86653.1 open reading frame X Not tested Type-I SCCmec Protein 6e-37 56
unnamed BAG06184.1 conserved hypothetical protein orfX Not tested Type-VII SCCmec Protein 6e-37 56
unnamed BAB47681.1 open reading frame X Not tested Type-III SCCmec Protein 6e-37 56
SAR0024 YP_039501.1 rRNA large subunit methyltransferase Not tested Type-II SCCmec Protein 9e-37 56
orfXhom BAB83497.1 open reading frame X Not tested SCC 12263 Protein 6e-37 55