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Name : mdeA (CBO0029) Accession : YP_001252577.1 Strain : Clostridium botulinum ATCC 3502 Genome accession: NC_009495 Putative virulence/resistance : Resistance Product : methionine gamma-lyase Function : - COG functional category : E : Amino acid transport and metabolism COG ID : COG0626 EC number : 4.4.1.11 Position : 35687 - 36889 bp Length : 1203 bp Strand : + Note : catalyzes the formation of methanethiol and 2-ocobutanoate from L-methionine DNA sequence : ATGGAAAATATAAAGAAAATGGGATTTGCGACAAAGGCTATACATGGAGGGCACATTGGAGATAAACAGTTTGGTTCATT GGCAACTCCAATATACCAAACATCTACATTTATATTTGATTCAGCAGAGCAAGGGGGTAGGCGATTTGCAGGAGAAGAAA GTGGATATATATATTCAAGATTAGGAAACCCTACATCTACAGAAGTAGAGAACAAGTTAGCTTTGTTAGAATGTGGAGAG GCAGCTGTTGTAGCTGCATCAGGTATGGGTGCTATAGCTGCATCTTTGTGGTCAGCTTTAAAATCAGGAGATCATGTAGT TGCATCAGATACTTTATATGGATGCACATTTGCACTATTAAATCATGGATTAACAAGATATGGAGTAGAGGTCACATTTG TAGACGTATCTAATTTAGATGAAGTAAGAAATGCATTGAAAGCAAATACTAAAGTAGTTTATTTAGAAACTCCAGCTAAT CCAACATTAAAGGTTACTGATATTAAACAAATATCAAATATGGTTCATGAAAATAATAAAGAATGTCTTGTATTTGTTGA TAATACATTTTGTACACCATATATACAAAGGCCATTACAATTAGGCGCTGATGTAGTTGTACATTCTGCTACTAAATATT TAAATGGACATGGAGATGTTATAGCTGGATTTGCAGTAGGAAAAGAGGAATTTATAAACCAAGTTAAATTATTTGGTATA AAAGACATGACAGGATCAGTTATAGGACCTTTTGAGGCATTTTTAATAATAAGAGGAATGAAAACATTACAATTAAGGAT GGAAAAACATTGTAAGAATGCTATGGAAGTAGCAAAGTTTTTAGAGTCCCATCCTGCAGTTAAAAAAGTTTATTATCCAG GATTAGAAAGTTTTGAATACTATGAGCTTGCTAAAAAGCAAATGAGTTTGCCTGGTGCTATGATTTCCTTTGAACTAAAA GGTGGAGTAGAAGAAGGTAAAGTAGTTATGAATAATGTTAAATTAGCTACTCTTGCAGTAAGTCTTGGGGATGCTGAAAC ATTAATTCAACATCCAGCATCAATGACACATTCACCTTATACAGCAGAAGAAAGAAAAGAAGCTGGTATAAGTGATGGGC TAGTAAGACTATCCGTAGGACTTGAAGATGTGGATGATATAATTAGTGATTTAAAACAAGCATTAGATTTAATAGTTAAG TAA Protein sequence : MENIKKMGFATKAIHGGHIGDKQFGSLATPIYQTSTFIFDSAEQGGRRFAGEESGYIYSRLGNPTSTEVENKLALLECGE AAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALLNHGLTRYGVEVTFVDVSNLDEVRNALKANTKVVYLETPAN PTLKVTDIKQISNMVHENNKECLVFVDNTFCTPYIQRPLQLGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGI KDMTGSVIGPFEAFLIIRGMKTLQLRMEKHCKNAMEVAKFLESHPAVKKVYYPGLESFEYYELAKKQMSLPGAMISFELK GGVEEGKVVMNNVKLATLAVSLGDAETLIQHPASMTHSPYTAEERKEAGISDGLVRLSVGLEDVDDIISDLKQALDLIVK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| SAR0460 | YP_039909.1 | Cys/Met metabolism PLP-dependent enzyme | Not tested | vSa¥á | Protein | 2e-69 | 44 |
| SAS0418 | YP_042545.1 | Cys/Met metabolism PLP-dependent enzyme | Not tested | vSa¥á | Protein | 1e-70 | 44 |
| metB | YP_281510.1 | cystathionine beta-lyase | Not tested | Not named | Protein | 2e-59 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| mdeA | YP_001252577.1 | methionine gamma-lyase | BAC0207 | Protein | 3e-107 | 54 |
