Gene Information

Name : mdeA (CBO0029)
Accession : YP_001252577.1
Strain : Clostridium botulinum ATCC 3502
Genome accession: NC_009495
Putative virulence/resistance : Resistance
Product : methionine gamma-lyase
Function : -
COG functional category : E : Amino acid transport and metabolism
COG ID : COG0626
EC number : 4.4.1.11
Position : 35687 - 36889 bp
Length : 1203 bp
Strand : +
Note : catalyzes the formation of methanethiol and 2-ocobutanoate from L-methionine

DNA sequence :
ATGGAAAATATAAAGAAAATGGGATTTGCGACAAAGGCTATACATGGAGGGCACATTGGAGATAAACAGTTTGGTTCATT
GGCAACTCCAATATACCAAACATCTACATTTATATTTGATTCAGCAGAGCAAGGGGGTAGGCGATTTGCAGGAGAAGAAA
GTGGATATATATATTCAAGATTAGGAAACCCTACATCTACAGAAGTAGAGAACAAGTTAGCTTTGTTAGAATGTGGAGAG
GCAGCTGTTGTAGCTGCATCAGGTATGGGTGCTATAGCTGCATCTTTGTGGTCAGCTTTAAAATCAGGAGATCATGTAGT
TGCATCAGATACTTTATATGGATGCACATTTGCACTATTAAATCATGGATTAACAAGATATGGAGTAGAGGTCACATTTG
TAGACGTATCTAATTTAGATGAAGTAAGAAATGCATTGAAAGCAAATACTAAAGTAGTTTATTTAGAAACTCCAGCTAAT
CCAACATTAAAGGTTACTGATATTAAACAAATATCAAATATGGTTCATGAAAATAATAAAGAATGTCTTGTATTTGTTGA
TAATACATTTTGTACACCATATATACAAAGGCCATTACAATTAGGCGCTGATGTAGTTGTACATTCTGCTACTAAATATT
TAAATGGACATGGAGATGTTATAGCTGGATTTGCAGTAGGAAAAGAGGAATTTATAAACCAAGTTAAATTATTTGGTATA
AAAGACATGACAGGATCAGTTATAGGACCTTTTGAGGCATTTTTAATAATAAGAGGAATGAAAACATTACAATTAAGGAT
GGAAAAACATTGTAAGAATGCTATGGAAGTAGCAAAGTTTTTAGAGTCCCATCCTGCAGTTAAAAAAGTTTATTATCCAG
GATTAGAAAGTTTTGAATACTATGAGCTTGCTAAAAAGCAAATGAGTTTGCCTGGTGCTATGATTTCCTTTGAACTAAAA
GGTGGAGTAGAAGAAGGTAAAGTAGTTATGAATAATGTTAAATTAGCTACTCTTGCAGTAAGTCTTGGGGATGCTGAAAC
ATTAATTCAACATCCAGCATCAATGACACATTCACCTTATACAGCAGAAGAAAGAAAAGAAGCTGGTATAAGTGATGGGC
TAGTAAGACTATCCGTAGGACTTGAAGATGTGGATGATATAATTAGTGATTTAAAACAAGCATTAGATTTAATAGTTAAG
TAA

Protein sequence :
MENIKKMGFATKAIHGGHIGDKQFGSLATPIYQTSTFIFDSAEQGGRRFAGEESGYIYSRLGNPTSTEVENKLALLECGE
AAVVAASGMGAIAASLWSALKSGDHVVASDTLYGCTFALLNHGLTRYGVEVTFVDVSNLDEVRNALKANTKVVYLETPAN
PTLKVTDIKQISNMVHENNKECLVFVDNTFCTPYIQRPLQLGADVVVHSATKYLNGHGDVIAGFAVGKEEFINQVKLFGI
KDMTGSVIGPFEAFLIIRGMKTLQLRMEKHCKNAMEVAKFLESHPAVKKVYYPGLESFEYYELAKKQMSLPGAMISFELK
GGVEEGKVVMNNVKLATLAVSLGDAETLIQHPASMTHSPYTAEERKEAGISDGLVRLSVGLEDVDDIISDLKQALDLIVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SAR0460 YP_039909.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 2e-69 44
SAS0418 YP_042545.1 Cys/Met metabolism PLP-dependent enzyme Not tested vSa¥á Protein 1e-70 44
metB YP_281510.1 cystathionine beta-lyase Not tested Not named Protein 2e-59 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
mdeA YP_001252577.1 methionine gamma-lyase BAC0207 Protein 3e-107 54