Gene Information

Name : leuS (CBO0186)
Accession : YP_001252730.1
Strain : Clostridium botulinum ATCC 3502
Genome accession: NC_009495
Putative virulence/resistance : Unknown
Product : leucyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG0495
EC number : 6.1.1.4
Position : 223757 - 226198 bp
Length : 2442 bp
Strand : -
Note : leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl

DNA sequence :
ATGGGAAACTATAGTACTAAAATCGACGAGAAATGGCAAAAAAAATGGGAAGAAAACTCTCTATACAAATTTAATAATAA
AAATTTAGATAAAAAATTATATGTACTTGAAATGTTTTCTTATCCATCAGGTAGTAAATTACATGCAGGACACTGGTTTA
ACTATGGGCCTGTAGACTCTTGGGCAAGATTTAAAAGAATGCAAGGTTACAATGTTTTTCAACCTATGGGTTTTGATGCT
TTTGGTCTTCCAGCTGAAAACTATGCAATAAAAACTGGTATACATCCAAAGGATTCTACCTTTAAAAATATTGAAACTAT
GGAAACTCAATTAAAAGCCATGGGTGCCATGTTCAACTGGGAAAATGAAGTTATAACTTGTTCACCAGACTACTATAAAT
GGACTCAATGGTTATTTTTAAAACTTTACGAAAAGGGTTTAGCTTATAAAAAGAAAGCACCTGTAAATTGGTGCCCAAGT
TGTAATACTGTTCTTGCTAATGAACAGGTTTTAGATGGAAAATGTGAAAGATGTGATAGCAATGTTGATAAAAAAAATCT
AGAGCAATGGTTCTTAAAGATAACTGACTATGCTGATGAATTATTAGAAAAACTCGATGAATTAGATTGGCCTGAAAAAA
CTAAGGCTATGCAAAAACACTGGATTGGAAAATCTGTAGGTGCTGAAGTTACATTTAATGTAGCAGATTCAGACTTGAGC
TTTAATGTATTTACTACAAGGGTAGATACTTTATTTGGAGTTACTTATGTAGTTTTAGCTCCTGAAAATGATTTAGTAGA
TAAGCTAACTACTCCTGAAAACAAAGCTGAAGTTGAATCATATAAAACTCAAGCTAAAAATCAATCTGATATAGAAAGAC
AATCTATAACTCGAGAAAAAACTGGTGTATTCTCAGGCTCTTATGCCATAAATCCTATAAACGGTAAAAAAGTACCTATA
TGGATAGGAGATTATGTATTAAATACCTATGGAACAGGTTGTGTAATGGCAGTTCCTGCCCATGATGAAAGAGATTTCGC
TTTTGCTACTAAATATAACCTTCCTATAGAAAGAGTTATAGAAGGCGGTGATTCTCTTCCTTATACAGAATATGGTGGAA
TGGTTAATAGTGGAGAATTCGATGGTTTATTGGGAAATGAGGCTAAAGAAGCTGTAATTTCTAAGTTAGAGTCTATGAAT
CTTGGTAGAAAGAAAATAAATTATAGATTAAGAGATTGGCTTGTATCAAGACAAAGATATTGGGGAGCACCTATTCCTAT
AATTTATTGTGAAAAATGTGGTACAGTGGAAGTTCCTATAGAACAGCTCCCTGTAGAATTACCATATAATGTAGAATTCT
CTCCAGATGGGAAATCACCTTTAGGTAAATGTGATGACTTTATAAATACAACTTGCCCAAAATGTGGTGGTCCAGCAAAA
AGAGAAGCGGACACATTAGATACCTTTGTATGTTCTTCTTGGTACTATTTAAGATATCCAGATAATAATAACGAAAAAGA
TGCTTTTAATCCTGAATTAATAAATAAAATGCTTCCAGTTGATAAATATGTTGGCGGTCCTGAACACGCTTGTATGCATC
TTTTATATGCTAGATTTATAACTAAAGCTTTAAGAGATATGGGGTATCTAAACTTTGATGAACCATTCTTATCTCTTACT
CATCAAGGATTAATATTAGGACCTGATGGCTTAAAAATGAGTAAATCAAAAGGAAATACTATATCTCCTGATGATTATAT
AAAAGAATTCGGTGCAGATGTATTTAGAATGTATTTAATGTTTGGATTTGATTATACAGAAGGCGGCGCTTGGAGTGACG
ATGCTATAAAATCCATAGGTAAATTTGTAGACAGAGTAGAAAGAATATTAGAAAATGCTAGAGAAGAAATAAAAAATTCT
AAAGATAATAAATCTACTATGGATAAAGATGAGAAAGAATTAAACTACGTAAGGCATCATAGCATTAAATCTATAACTGA
AGATATAGATAAAATGCAGTTCAATACTTCCATAGCAAGATTAATGGAATTTACAAATGCTCTATCTAAATATCTTGGTA
TTGATGCTATTAAAAATGCTTTATTCTTGAGAGAAAGTATAATTGACTTTATAACATTATTAGCACCTTTTGCTCCTCAT
TTTGCTGAAGAACAATGGAAACTTATAGGCATTAATTCTTCCATTTTCAATGAAAAATGGCCTGAATTTGACCCTAAAGC
CCTAATAAAAGATGAAGTTGAAATAGCCGTACAAGTAAATGGGAAAATAAGAGCTAAAATAAATATATCTACTAGTTCAT
CAGAAGATGAAATAAAAGAATCAGCATTAAATAATGAGGATATTAAAAATTCTATAGGTGATAAAGAAATTAAAAAAGTT
ATAGTAATAAAAAATAGATTAGTTAATATAGTTGCTAAATAA

Protein sequence :
MGNYSTKIDEKWQKKWEENSLYKFNNKNLDKKLYVLEMFSYPSGSKLHAGHWFNYGPVDSWARFKRMQGYNVFQPMGFDA
FGLPAENYAIKTGIHPKDSTFKNIETMETQLKAMGAMFNWENEVITCSPDYYKWTQWLFLKLYEKGLAYKKKAPVNWCPS
CNTVLANEQVLDGKCERCDSNVDKKNLEQWFLKITDYADELLEKLDELDWPEKTKAMQKHWIGKSVGAEVTFNVADSDLS
FNVFTTRVDTLFGVTYVVLAPENDLVDKLTTPENKAEVESYKTQAKNQSDIERQSITREKTGVFSGSYAINPINGKKVPI
WIGDYVLNTYGTGCVMAVPAHDERDFAFATKYNLPIERVIEGGDSLPYTEYGGMVNSGEFDGLLGNEAKEAVISKLESMN
LGRKKINYRLRDWLVSRQRYWGAPIPIIYCEKCGTVEVPIEQLPVELPYNVEFSPDGKSPLGKCDDFINTTCPKCGGPAK
READTLDTFVCSSWYYLRYPDNNNEKDAFNPELINKMLPVDKYVGGPEHACMHLLYARFITKALRDMGYLNFDEPFLSLT
HQGLILGPDGLKMSKSKGNTISPDDYIKEFGADVFRMYLMFGFDYTEGGAWSDDAIKSIGKFVDRVERILENAREEIKNS
KDNKSTMDKDEKELNYVRHHSIKSITEDIDKMQFNTSIARLMEFTNALSKYLGIDAIKNALFLRESIIDFITLLAPFAPH
FAEEQWKLIGINSSIFNEKWPEFDPKALIKDEVEIAVQVNGKIRAKINISTSSSEDEIKESALNNEDIKNSIGDKEIKKV
IVIKNRLVNIVAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
leuS YP_281511.2 leucyl-tRNA synthetase Not tested Not named Protein 6e-169 46