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Name : leuS (CBO0186) Accession : YP_001252730.1 Strain : Clostridium botulinum ATCC 3502 Genome accession: NC_009495 Putative virulence/resistance : Unknown Product : leucyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG0495 EC number : 6.1.1.4 Position : 223757 - 226198 bp Length : 2442 bp Strand : - Note : leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl DNA sequence : ATGGGAAACTATAGTACTAAAATCGACGAGAAATGGCAAAAAAAATGGGAAGAAAACTCTCTATACAAATTTAATAATAA AAATTTAGATAAAAAATTATATGTACTTGAAATGTTTTCTTATCCATCAGGTAGTAAATTACATGCAGGACACTGGTTTA ACTATGGGCCTGTAGACTCTTGGGCAAGATTTAAAAGAATGCAAGGTTACAATGTTTTTCAACCTATGGGTTTTGATGCT TTTGGTCTTCCAGCTGAAAACTATGCAATAAAAACTGGTATACATCCAAAGGATTCTACCTTTAAAAATATTGAAACTAT GGAAACTCAATTAAAAGCCATGGGTGCCATGTTCAACTGGGAAAATGAAGTTATAACTTGTTCACCAGACTACTATAAAT GGACTCAATGGTTATTTTTAAAACTTTACGAAAAGGGTTTAGCTTATAAAAAGAAAGCACCTGTAAATTGGTGCCCAAGT TGTAATACTGTTCTTGCTAATGAACAGGTTTTAGATGGAAAATGTGAAAGATGTGATAGCAATGTTGATAAAAAAAATCT AGAGCAATGGTTCTTAAAGATAACTGACTATGCTGATGAATTATTAGAAAAACTCGATGAATTAGATTGGCCTGAAAAAA CTAAGGCTATGCAAAAACACTGGATTGGAAAATCTGTAGGTGCTGAAGTTACATTTAATGTAGCAGATTCAGACTTGAGC TTTAATGTATTTACTACAAGGGTAGATACTTTATTTGGAGTTACTTATGTAGTTTTAGCTCCTGAAAATGATTTAGTAGA TAAGCTAACTACTCCTGAAAACAAAGCTGAAGTTGAATCATATAAAACTCAAGCTAAAAATCAATCTGATATAGAAAGAC AATCTATAACTCGAGAAAAAACTGGTGTATTCTCAGGCTCTTATGCCATAAATCCTATAAACGGTAAAAAAGTACCTATA TGGATAGGAGATTATGTATTAAATACCTATGGAACAGGTTGTGTAATGGCAGTTCCTGCCCATGATGAAAGAGATTTCGC TTTTGCTACTAAATATAACCTTCCTATAGAAAGAGTTATAGAAGGCGGTGATTCTCTTCCTTATACAGAATATGGTGGAA TGGTTAATAGTGGAGAATTCGATGGTTTATTGGGAAATGAGGCTAAAGAAGCTGTAATTTCTAAGTTAGAGTCTATGAAT CTTGGTAGAAAGAAAATAAATTATAGATTAAGAGATTGGCTTGTATCAAGACAAAGATATTGGGGAGCACCTATTCCTAT AATTTATTGTGAAAAATGTGGTACAGTGGAAGTTCCTATAGAACAGCTCCCTGTAGAATTACCATATAATGTAGAATTCT CTCCAGATGGGAAATCACCTTTAGGTAAATGTGATGACTTTATAAATACAACTTGCCCAAAATGTGGTGGTCCAGCAAAA AGAGAAGCGGACACATTAGATACCTTTGTATGTTCTTCTTGGTACTATTTAAGATATCCAGATAATAATAACGAAAAAGA TGCTTTTAATCCTGAATTAATAAATAAAATGCTTCCAGTTGATAAATATGTTGGCGGTCCTGAACACGCTTGTATGCATC TTTTATATGCTAGATTTATAACTAAAGCTTTAAGAGATATGGGGTATCTAAACTTTGATGAACCATTCTTATCTCTTACT CATCAAGGATTAATATTAGGACCTGATGGCTTAAAAATGAGTAAATCAAAAGGAAATACTATATCTCCTGATGATTATAT AAAAGAATTCGGTGCAGATGTATTTAGAATGTATTTAATGTTTGGATTTGATTATACAGAAGGCGGCGCTTGGAGTGACG ATGCTATAAAATCCATAGGTAAATTTGTAGACAGAGTAGAAAGAATATTAGAAAATGCTAGAGAAGAAATAAAAAATTCT AAAGATAATAAATCTACTATGGATAAAGATGAGAAAGAATTAAACTACGTAAGGCATCATAGCATTAAATCTATAACTGA AGATATAGATAAAATGCAGTTCAATACTTCCATAGCAAGATTAATGGAATTTACAAATGCTCTATCTAAATATCTTGGTA TTGATGCTATTAAAAATGCTTTATTCTTGAGAGAAAGTATAATTGACTTTATAACATTATTAGCACCTTTTGCTCCTCAT TTTGCTGAAGAACAATGGAAACTTATAGGCATTAATTCTTCCATTTTCAATGAAAAATGGCCTGAATTTGACCCTAAAGC CCTAATAAAAGATGAAGTTGAAATAGCCGTACAAGTAAATGGGAAAATAAGAGCTAAAATAAATATATCTACTAGTTCAT CAGAAGATGAAATAAAAGAATCAGCATTAAATAATGAGGATATTAAAAATTCTATAGGTGATAAAGAAATTAAAAAAGTT ATAGTAATAAAAAATAGATTAGTTAATATAGTTGCTAAATAA Protein sequence : MGNYSTKIDEKWQKKWEENSLYKFNNKNLDKKLYVLEMFSYPSGSKLHAGHWFNYGPVDSWARFKRMQGYNVFQPMGFDA FGLPAENYAIKTGIHPKDSTFKNIETMETQLKAMGAMFNWENEVITCSPDYYKWTQWLFLKLYEKGLAYKKKAPVNWCPS CNTVLANEQVLDGKCERCDSNVDKKNLEQWFLKITDYADELLEKLDELDWPEKTKAMQKHWIGKSVGAEVTFNVADSDLS FNVFTTRVDTLFGVTYVVLAPENDLVDKLTTPENKAEVESYKTQAKNQSDIERQSITREKTGVFSGSYAINPINGKKVPI WIGDYVLNTYGTGCVMAVPAHDERDFAFATKYNLPIERVIEGGDSLPYTEYGGMVNSGEFDGLLGNEAKEAVISKLESMN LGRKKINYRLRDWLVSRQRYWGAPIPIIYCEKCGTVEVPIEQLPVELPYNVEFSPDGKSPLGKCDDFINTTCPKCGGPAK READTLDTFVCSSWYYLRYPDNNNEKDAFNPELINKMLPVDKYVGGPEHACMHLLYARFITKALRDMGYLNFDEPFLSLT HQGLILGPDGLKMSKSKGNTISPDDYIKEFGADVFRMYLMFGFDYTEGGAWSDDAIKSIGKFVDRVERILENAREEIKNS KDNKSTMDKDEKELNYVRHHSIKSITEDIDKMQFNTSIARLMEFTNALSKYLGIDAIKNALFLRESIIDFITLLAPFAPH FAEEQWKLIGINSSIFNEKWPEFDPKALIKDEVEIAVQVNGKIRAKINISTSSSEDEIKESALNNEDIKNSIGDKEIKKV IVIKNRLVNIVAK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| leuS | YP_281511.2 | leucyl-tRNA synthetase | Not tested | Not named | Protein | 6e-169 | 46 |
