Gene Information

Name : BBta_4978 (BBta_4978)
Accession : YP_001240891.1
Strain : Bradyrhizobium sp. BTAi1
Genome accession: NC_009485
Putative virulence/resistance : Unknown
Product : IS66 family insertion sequence transposase protein
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG3436
EC number : -
Position : 5213392 - 5215041 bp
Length : 1650 bp
Strand : +
Note : Evidence: Function proposed based on presence of conserved amino acid motif, structural feature or limited homology

DNA sequence :
ATGAATGCTGATCGCGACGCTGTTCCGGATGACGTTGGCGCCCTGAAGGAGGCGCTGGCAGTCGAGCGCGCGAAGGGTTT
GGAGATCGCCGCTGAACTCGCGGTCGCCCGCGCGAAAGCGTCGGAAGACGAAGCGCTGATCGCCCAGCAGCAGTTGCAGA
TCGCCAAGCTGAAACATCAGATCTATGGGCCACGGTCGGAACGTTCGGCGCGGCTGATCGAGCAGTTGGCGCTGACGTTC
GAAGAGCTGGAAGCCGACGCCACCGAGGACGAGCTGGCGGCGGAACGGGCCGTGGCGAAGACGACGACAGTGCGTGGATT
TACACGCAAGCGCCCCGAGCGTCAGACCTTCCCCGAGCATCTGCCACGGGAACGGGTGGTGATCGATCCGCCGGCAGCTT
GTGAATGTTGCGGCAGCAATCGCCTTCGCAAGCTCGGCGAGGACGTGACGCGCACGCTGGAGGTAGTGCCGCGCCAGTGG
AAGGTGATCGAGACGGTACGGGAGAAGTTCTCCTGCCGCGATTGCGAGAAGATCAGCCAGGCCCCGGCGCCGTTCCATGC
CATCCCGCGGGGATGGGCCGGCCCGAGCCTCCTGGCCATGATCATGTTCGAGAAGTTCGGCCAGCATCAGCCCTTGAACC
GCCAGGCCGAGCGGTATGCCCTGGAAGGGGTGCCGATCGCGCTATCAACCATGGCCGATGCCGTGGGGGCGGTCTGTACG
TCGCTGGATCCCCTGCTGCGCCTGGTGGAAGCCCATGTTATGGCAGCCGAGCGCCTTCATGCCGACGATACGACGGTGCC
GGTCCTGGCCAAGGGCAAGACCGACACGGGACGGTGCTGGATCTACGTTCGGGACGACCGGCCGTTTGGGGGCGCGGGTC
CACCAGCAGCGATGTTCTACTACTCACGCGACCGCAAGGGCGAGCATCCGCAGGGGCATCTGGCCCGGTATGCCGGCATC
CTGCAGGCCGACGCCTATGACGGCTACAACCAGCTCTATCTGGCCGGGCGCCAGCCCGGACCGATCCGGGAGGCTGCCTG
CTGGTCACATGGAAGGCGCCCGTTCTTTGCCATGGCCGACATCGAAGAGAATGCGCGGCGCAAGGGCGCCGGAAAGAAGG
AAATCCCGCTCTCTCCGATCGCGATCGAGGTCGTGCGGCGGATCGATGAGCTGTTCGAGATCGAACGTTCCATCAATGGC
AGGAGCCCCGAAGGGCGTCTTCAGGTGCGACAGATGCTGAGCCGGCCTCTGGTGGAGGACCTTCAGGTCTACATGCGCGA
ACAGCTCGCCAAACTGTCCCGCGGTCACGACCTGGCCAAGGCGTTCAACTATATCCTGAAGCGATGGGCAAGCTTCACGC
TGTTCCTGGACGATGGACGCGTGTGTCTCTCCAACAATGCCGCCGAACGAGGCTTGCGAGGCATTGCTCTTGGACGAAAA
TCCTGGCTGTTCTGCGGGTCTGATCGCGGAGGGCGACGTGCCGCAGCCATGTACAGCTTGATCGTCACGGCAAAAATGAA
CGGCATCGATCCGCAGGCGTGGCTTACGGATATCCTCGCTCGCATCGCCGCCCATCCGGCTCATCGGCTGGACGAGCTTC
TGCCCTGGAATTGGACGCCAGCACCAACAATCTCTGCTCAAGCCGCATGA

Protein sequence :
MNADRDAVPDDVGALKEALAVERAKGLEIAAELAVARAKASEDEALIAQQQLQIAKLKHQIYGPRSERSARLIEQLALTF
EELEADATEDELAAERAVAKTTTVRGFTRKRPERQTFPEHLPRERVVIDPPAACECCGSNRLRKLGEDVTRTLEVVPRQW
KVIETVREKFSCRDCEKISQAPAPFHAIPRGWAGPSLLAMIMFEKFGQHQPLNRQAERYALEGVPIALSTMADAVGAVCT
SLDPLLRLVEAHVMAAERLHADDTTVPVLAKGKTDTGRCWIYVRDDRPFGGAGPPAAMFYYSRDRKGEHPQGHLARYAGI
LQADAYDGYNQLYLAGRQPGPIREAACWSHGRRPFFAMADIEENARRKGAGKKEIPLSPIAIEVVRRIDELFEIERSING
RSPEGRLQVRQMLSRPLVEDLQVYMREQLAKLSRGHDLAKAFNYILKRWASFTLFLDDGRVCLSNNAAERGLRGIALGRK
SWLFCGSDRGGRRAAAMYSLIVTAKMNGIDPQAWLTDILARIAAHPAHRLDELLPWNWTPAPTISAQAA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed AAL57570.1 unknown Not tested LEE Protein 3e-82 47
st57 CAC81895.1 ST57 protein Not tested LEE II Protein 1e-81 46
Z1570 NP_287074.1 hypothetical protein Not tested TAI Protein 4e-81 46
unnamed CAC39285.1 hypothetical protein Not tested LPA Protein 6e-82 46
Z4337 NP_289562.1 hypothetical protein Not tested OI-122 Protein 8e-82 46
unnamed AAC31494.1 L0015 Not tested LEE Protein 6e-82 46
Z5098 NP_290249.1 prophage-associated protein Not tested LEE Protein 8e-82 46
tnp AEA34686.1 transposase Not tested Not named Protein 5e-82 46
ECs4547 NP_312574.1 hypothetical protein Not tested LEE Protein 4e-82 46
unnamed ACU09439.1 IS66 family element transposase Not tested LEE Protein 3e-82 46
Z1131 NP_286666.1 hypothetical protein Not tested TAI Protein 4e-81 46
BCAM0248 YP_002232880.1 putative transposase Not tested BcenGI11 Protein 7e-82 46
unnamed CAI43806.1 hypothetical protein Not tested LEE Protein 9e-82 46
aec53 AAW51736.1 Aec53 Not tested AGI-3 Protein 1e-75 44
s0025 CAD33772.1 IS66-like transposase Not tested PAI I 536 Protein 4e-61 43
Z4340 NP_289564.1 hypothetical protein Not tested OI-122 Protein 5e-75 43
Z4317 NP_289543.1 hypothetical protein Not tested OI-122 Protein 6e-64 43
Z1161 NP_286696.1 hypothetical protein Not tested TAI Protein 2e-73 43
Z1600 NP_287104.1 hypothetical protein Not tested TAI Protein 2e-73 43
ECO103_3554 YP_003223421.1 hypothetical protein Not tested LEE Protein 2e-63 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
BBta_4978 YP_001240891.1 IS66 family insertion sequence transposase protein VFG0793 Protein 3e-82 46
BBta_4978 YP_001240891.1 IS66 family insertion sequence transposase protein VFG1513 Protein 3e-61 43