Gene Information

Name : pyk (LRC283)
Accession : YP_684589.1
Strain : Methanocella arvoryzae MRE50
Genome accession: NC_009464
Putative virulence/resistance : Virulence
Product : pyruvate kinase (pyruvate phosphotransferase)
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2991591 - 2993342 bp
Length : 1752 bp
Strand : +
Note : identical to orf1 of fosmid clone 3D10mr4

DNA sequence :
ATGAGGAAGACCAAGATCGTTTGTACTATCGGCCCTGCGTGCGATAGCCAGGACATGCTGGAAAAGCTGGCCGTAGCCGG
AATGAACGTAGCCAGGCTGAACATGTCTCATGCAGATCACGAGCATACTGTTCAGACTATCAATAACATTCGCATGGTTT
CAGAGGCGATAGGCAAGCCGATCGGCATACTGATGGACCTCCAGGGCCCGAAGATCCGGGTCGGCACCCTGCAGCAGCCG
GCAAACCTGAAGCCGGGAGGAACTTTTACCCTGACCACCAGAGACGTGCCTGGAGACAGCCAGGAAGTCAACGTCCCCTT
CAAAGAGCTTCCCCAGTCGGTGAGCACGGGCCAGACGCTGCTCCTCGACGACGGGCTGATCGAGCTAAAGGTGGACGCTG
TCACCGAGACGGATATACGGACAAAAGTAGTCAGGGGCGGAGAGCTGAAATCCAAAAAAGGCATCAACCTGCCCCAGAGC
ACGATCAGGATTCCTTCGATCACCGAAAAAGACGTAAGAGATCTCGAGTTCGGCATCGAGCATGAAGTCGATATGATCGC
CATGTCTTTCGTTCGAAAGCCTCAGGACGTCCTCGATCTGAGGAAAAAGATCGAAGACAATGACTCCGATATCCCCATTA
TTTCGAAGATCGAGAAGCACGAGGCGGTCAAGAACATCGACGGCATCATAGACGTTGTCGACGGCGTGATGGTTGCCCGC
GGCGATCTGGGGATCGAGATTCCCATGGCCGAGGTGCCAATCGTACAGAAAATGATCATCTCGAAATGCATTGCCCGAGG
GATCCCGGTTATTACGGCCACCCAGATGCTGGACTCTATGATCAGGAACCCGATACCTACCCGGGCAGAGGCTACGGACG
TGGCCAACGCAGTTTTCGACGGCACAGACGCCCTGATGCTGTCCGGAGAGACCGCCTTCGGCGAATATCCTGTCAAAGCC
GTAGAGACCATGGCCAGAATCGCGAAGTATACGGAAGAGTCTACTTATTACAAGCATGCGATAGCTGCAAAAGCCCCAAA
GCCCTCTCTATCAATGACGGATGCGGTCGCCCAGAGCACGACTGAGTCCGCCAGAGTACTCAAAGCGCAGGCGATCATCA
CCGCAACTCAGACCGGCTATTCCGCCAGGAAAGTCTCCAAATACCGGCCGCAGCTGCCCATTCTTGCAGTGACCAACGAT
CCGAAGGTGGTAAACCGTCTCACGCTTTCGTGGGCGGTCTTACCCTTACTGATCGGCAGCCCGGGGAACCTCGACGAGCT
GATTCAGGACTCTGTGGACGCCTGCCTGCAAAAGGGCTACGTCAAGAACGGGGACCTGGTCGTGATAACGGCTGGCGTGA
TGACCGGCATTCCTGGCGGCACTAACATCATGAAAATCCACGTTGTGGCAAAAGAGCTTGCCAGAGGCATAGGCGTGGGC
AAGGACATCGTGAAAGGCGTGGCCAGGGTGATTAAGTCTCCCTCCGAGTTCACAAATATCCGTAGCGGCGATATTCTGGT
GATCAAAGAGGTCGATGTCGATCACATAGACGACGTGAAGCCGGCGCGTGCGATAATCTCGGAGGAAACCGGATTGACGT
CCTACAGTGCTATTATAGGCAGAGAGCTCAATATCCCCGTTGTAGTGGGCATAAAGAACGCTACGAGAATTATTCAGGAT
GGCAGCACTATTACTGTTGACTCAGTGCGAGGCATGGTCTACGAGGGATCCATCAATCTGCCGGGCGAATAA

Protein sequence :
MRKTKIVCTIGPACDSQDMLEKLAVAGMNVARLNMSHADHEHTVQTINNIRMVSEAIGKPIGILMDLQGPKIRVGTLQQP
ANLKPGGTFTLTTRDVPGDSQEVNVPFKELPQSVSTGQTLLLDDGLIELKVDAVTETDIRTKVVRGGELKSKKGINLPQS
TIRIPSITEKDVRDLEFGIEHEVDMIAMSFVRKPQDVLDLRKKIEDNDSDIPIISKIEKHEAVKNIDGIIDVVDGVMVAR
GDLGIEIPMAEVPIVQKMIISKCIARGIPVITATQMLDSMIRNPIPTRAEATDVANAVFDGTDALMLSGETAFGEYPVKA
VETMARIAKYTEESTYYKHAIAAKAPKPSLSMTDAVAQSTTESARVLKAQAIITATQTGYSARKVSKYRPQLPILAVTND
PKVVNRLTLSWAVLPLLIGSPGNLDELIQDSVDACLQKGYVKNGDLVVITAGVMTGIPGGTNIMKIHVVAKELARGIGVG
KDIVKGVARVIKSPSEFTNIRSGDILVIKEVDVDHIDDVKPARAIISEETGLTSYSAIIGRELNIPVVVGIKNATRIIQD
GSTITVDSVRGMVYEGSINLPGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-84 46
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-84 46
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 6e-85 46
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 6e-84 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pyk YP_684589.1 pyruvate kinase (pyruvate phosphotransferase) VFG0479 Protein 1e-84 46