Gene Information

Name : lysS (Pmen_3370)
Accession : YP_001188854.1
Strain : Pseudomonas mendocina ymp
Genome accession: NC_009439
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3718517 - 3720034 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGAGCGACCAACAACTCGACCAGCCCAGCCTCAGCCACGAAGAGCGTCAGCACGAAGAAAACAAGCTGATCGCCCAGCG
CAAGGAAAAGCTTGCCGCCGTGCGTGAGCAGGGCATTGCCTTCCCCAACGATTTCCGTCGCGACAGCCTGTGCGGCGAGC
TGCAGAAACAGTACGAGGGCAAGAGCAAGGAAGAGCTGGAGGCCGCTGCCATCCCGGTCAAGATCGCCGGACGCATCATG
CTCAACCGCGGCGCCTTCATGGTCCTGCAGGACACCTCCGGGCGCCTGCAGGTGTACGTTGACCGCAAGGGCCTGCCGGC
CGAAACCCTGGAAGCGATCAAGACCTGGGACCTGGGCGACATCATCGCCGCCGAAGGCACCCTGGCGCGTTCCGGCAAGG
GCGACCTGTACGTGAACATGACCAGCGTGCGCCTGCTGACCAAGTCGCTGCGCCCGCTGCCGGACAAGCACCACGGCCTG
ACCGACACCGAGCAGCGCTACCGTCAGCGTTACGTCGACCTGATCGTCAACGAGGAAACCCGCCACACCTTCCGCGTACG
TTCGCAGGTGATCGCGCACATCCGCCGTTTCCTCAACGAGCGCGGCTTCCTCGAAGTGGAAACCCCGATGCTGCAGACCA
TTCCCGGCGGCGCGGCGGCCAAGCCCTTCGAGACCCATCACAACGCCTTGGACATGCAGATGTTCCTGCGCATCGCCCCG
GAGCTGTACCTCAAGCGGCTTGTTGTTGGTGGTTTTGAAAAAGTGTTCGAGATCAACCGCAACTTCCGTAACGAAGGCGT
CTCGACCCGGCACAACCCCGAGTTCACCATGCTCGAGTTCTACCAGGCCTACGCCGACTACCGCGACAACATGGACCTGA
CCGAGGAGCTGTTCCGCGAGCTGGCCCTGGCGGTGCTGGGCACCACCGACGTGCCCTACGGCGACAAGGTGTTCCATTTC
GGCGAGCCCTTCGTGCGCCTGTCGGTGTACGACTCCATCCTCAAGTACAACCCGGACATCACCGAGGCTGACCTCAATGA
CGTCGACAAGGCCCGCGCCATCGCCAAGAAGGCCGGCGCCAAGGTGCTCGGCCACGAAGGCCTGGGCAAGCTGCAGGTGA
TGATTTTCGAGGAGCTGGTGGAGAGCAAGCTGGAGCAGCCGCACTTCATCACCGAGTACCCCTTCGAGGTCTCGCCGCTG
GCCCGTCGCAACGACCAGAACCCCGCCGTCACCGACCGCTTCGAGCTGTTCATCGGCGGCCGCGAGATCGCCAACGCCTA
TTCCGAGCTCAATGACGCCGAGGACCAGGCCGAGCGTTTCCACGCCCAGGTGGCCGAGAAGGACGCCGGCGACGACGAGG
CCATGCACTTCGACGCCGACTTCGTGCGCGCCCTGGAATACGGCATGCCGCCCACCGCAGGTGAAGGCATCGGCATCGAC
CGTCTGGTGATGCTGCTGACCAACTCGCCGTCGATCCGCGACGTCATCCTGTTCCCGCACATGCGTCCGCAGGCCTGA

Protein sequence :
MSDQQLDQPSLSHEERQHEENKLIAQRKEKLAAVREQGIAFPNDFRRDSLCGELQKQYEGKSKEELEAAAIPVKIAGRIM
LNRGAFMVLQDTSGRLQVYVDRKGLPAETLEAIKTWDLGDIIAAEGTLARSGKGDLYVNMTSVRLLTKSLRPLPDKHHGL
TDTEQRYRQRYVDLIVNEETRHTFRVRSQVIAHIRRFLNERGFLEVETPMLQTIPGGAAAKPFETHHNALDMQMFLRIAP
ELYLKRLVVGGFEKVFEINRNFRNEGVSTRHNPEFTMLEFYQAYADYRDNMDLTEELFRELALAVLGTTDVPYGDKVFHF
GEPFVRLSVYDSILKYNPDITEADLNDVDKARAIAKKAGAKVLGHEGLGKLQVMIFEELVESKLEQPHFITEYPFEVSPL
ARRNDQNPAVTDRFELFIGGREIANAYSELNDAEDQAERFHAQVAEKDAGDDEAMHFDADFVRALEYGMPPTAGEGIGID
RLVMLLTNSPSIRDVILFPHMRPQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-134 60
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 2e-80 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001188854.1 lysyl-tRNA synthetase VFG1668 Protein 6e-135 60