Gene Information

Name : fliI (Pmen_2822)
Accession : YP_001188309.1
Strain : Pseudomonas mendocina ymp
Genome accession: NC_009439
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 3098358 - 3099704 bp
Length : 1347 bp
Strand : -
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGCGCCTTGATCGGGTGAGTTTCGCCCGGCGTCTGGAGGGCTACACCGAGGCGATCAGCCTGCCCAGCCAGCCCATACT
CGAAGGCCGCCTGCTGCGCATGGTGGGCCTGACCCTGGAGGCAGAAGGCCTGCGCGCCGCCATCGGCAGCCGCTGCCTGG
TGATCAATGGCGACAGCTACCACCCCGTGCAGGTCGAGGCCGAGGTCATGGGCTTTTCCGGCGGCAAGATCTACCTGATG
CCCGTGGGCAGTCTGGCCGGGATTGCACCTGGCGCCCGCGTGGTGCCTCTGCCCGATGCCGGCCGCCTGCCGATGGGCAT
GTCAATGCTCGGCCGGGTGCTCGACGGCGCCGGCAGGGCGCTCGACGGCAAGGGCGGGATGAAGGCCGAGGACTGGGTGC
CGGTCGACGGGCCTGCCATCAATCCGCTCAATCGCGATCCCATCAGCCAGCCGCTGGATGTCGGCATTCGCACCATCAAC
GGCCTGCTCACCGTCGGACGCGGCCAGCGTCTGGGCCTGTTCGCCGGTACCGGCGTGGGCAAGTCGGTGCTGCTGGGCAT
GATGACCCGTTTCACCGAGGCCGAGATCATCGTCGTCGGCCTGATCGGCGAGCGTGGTCGCGAGGTCAAGGAGTTCATCG
ACGAGATTCTCGGCGCCGAAGGCCTCAAGCGTTCGGTGGTGGTGGCTTCGCCCGCTGACGATGCGCCATTGATGCGTCTG
CGCGCGGCGCAGTACTGCACGCGCATCGCCGAATATTTCCGCGACAAGGGCAAGAACGTGCTGCTGCTGATGGATTCGCT
GACCCGCTATGCCCAGGCGCAGCGCGAGATCGCCCTGGCCATCGGCGAGCCGCCGGCAACCAAGGGTTATCCGCCTTCGG
TGTTCGCCAAGCTGCCTCGGCTGGTCGAACGCGCCGGTAATGGCGAGGCGGGCGGTGGTTCGATCACGGCCTTCTACACG
GTATTGAGCGAGGGCGACGACCAGCAGGACCCCATCGCCGATGCGGCGCGCGGCGTACTCGACGGCCACTTCGTGCTGTC
GCGGCGGCTGGCGGAGGAGGGGCACTATCCGGCCATCGACATCGAATCGTCGATCAGCCGGGTGATGCCGCAGGTGGTAC
CGCCGGAGCAGCTCAAGCAGGCGCAGCGTTTCAAGCAACTCTGGTCGCGCTATCAGCAGAGCCGCGATCTGATCAGCGTC
GGCGCCTACGTGCCGGGCGGCGACGCCGACACCGATCTGGCCATCGCCCGGCAACCGCAGATGGCCCAGTACCTGCGCCA
GGGCCTCGGCGAAAGCGTGGACATGGCGCAGAGCCGCGAGCAACTGGCGGCGGTGCTGGGGGCGTGA

Protein sequence :
MRLDRVSFARRLEGYTEAISLPSQPILEGRLLRMVGLTLEAEGLRAAIGSRCLVINGDSYHPVQVEAEVMGFSGGKIYLM
PVGSLAGIAPGARVVPLPDAGRLPMGMSMLGRVLDGAGRALDGKGGMKAEDWVPVDGPAINPLNRDPISQPLDVGIRTIN
GLLTVGRGQRLGLFAGTGVGKSVLLGMMTRFTEAEIIVVGLIGERGREVKEFIDEILGAEGLKRSVVVASPADDAPLMRL
RAAQYCTRIAEYFRDKGKNVLLLMDSLTRYAQAQREIALAIGEPPATKGYPPSVFAKLPRLVERAGNGEAGGGSITAFYT
VLSEGDDQQDPIADAARGVLDGHFVLSRRLAEEGHYPAIDIESSISRVMPQVVPPEQLKQAQRFKQLWSRYQQSRDLISV
GAYVPGGDADTDLAIARQPQMAQYLRQGLGESVDMAQSREQLAAVLGA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-90 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-90 49
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 7e-93 49
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 7e-93 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-92 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-93 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-92 49
lscN AAO18044.1 LscN Virulence TTSS locus Protein 5e-84 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-77 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-77 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-77 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-77 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-77 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-90 48
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 9e-91 48
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-73 48
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-73 48
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 4e-73 48
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-73 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 9e-87 48
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-78 47
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-69 47
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 7e-70 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 4e-67 46
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-72 46
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-69 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 6e-69 45
escN AAK26715.1 EscN Virulence LEE Protein 4e-73 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 4e-74 45
escN AAL57542.1 EscN Virulence LEE Protein 4e-73 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-73 45
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-73 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-73 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-73 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 9e-66 45
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 9e-66 45
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 3e-75 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-70 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-70 44
escN AAC31513.1 L0034 Virulence LEE Protein 2e-74 43
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-74 43
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-74 43
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-74 43
escN AAC38383.1 EscN Virulence LEE Protein 2e-74 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-64 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 6e-64 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-64 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-64 42
invC AAS66861.1 InvC Not tested SSR-2 Protein 3e-66 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001188309.1 flagellum-specific ATP synthase VFG1254 Protein 0.0 89
fliI YP_001188309.1 flagellum-specific ATP synthase VFG2499 Protein 1e-123 62
fliI YP_001188309.1 flagellum-specific ATP synthase VFG2331 Protein 2e-120 60
fliI YP_001188309.1 flagellum-specific ATP synthase VFG0515 Protein 8e-78 49
fliI YP_001188309.1 flagellum-specific ATP synthase VFG0192 Protein 5e-88 48
fliI YP_001188309.1 flagellum-specific ATP synthase VFG0048 Protein 3e-89 47
fliI YP_001188309.1 flagellum-specific ATP synthase VFG0390 Protein 7e-83 46
fliI YP_001188309.1 flagellum-specific ATP synthase VFG2144 Protein 2e-86 45
fliI YP_001188309.1 flagellum-specific ATP synthase VFG0812 Protein 9e-75 43
fliI YP_001188309.1 flagellum-specific ATP synthase VFG0730 Protein 1e-74 43
fliI YP_001188309.1 flagellum-specific ATP synthase VFG0555 Protein 2e-64 42
fliI YP_001188309.1 flagellum-specific ATP synthase VFG1008 Protein 2e-62 42