Gene Information

Name : Sputcn32_3473 (Sputcn32_3473)
Accession : YP_001184982.1
Strain : Shewanella putrefaciens CN-32
Genome accession: NC_009438
Putative virulence/resistance : Virulence
Product : FliI/YscN family ATPase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.15
Position : 4036667 - 4038016 bp
Length : 1350 bp
Strand : -
Note : KEGG: sdn:Sden_3656 ATPase FliI/YscN; TIGRFAM: ATPase, FliI/YscN family; PFAM: H+-transporting two-sector ATPase, alpha/beta subunit, central region; H+-transporting two-sector ATPase, alpha/beta subunit domain protein; SMART: AAA ATPase

DNA sequence :
ATGAGGCCGATATTGGAGGCTGATTTTTTCCAAGACGATTGGCCACGGGTACCGGTTGCTCGAGTTTATGGACGTCTTAT
CCGAGTCAATGGTTTATTGCTGGAAGCCGTTGGTTGTCAGCTTGGCATGGGCGATCGTTGTCAGATAGAAGGACGTCATG
GTCGTTTAATTGAGGCTGAGGTCGTTGGTTTTCACCGAGATGTACTTTGCCTTATGCCTATCGAACCTACTCAGGGGTTG
ATGCCGGGGGCGAGAGTATTGCCTATTACCCATGAGCAAACGATTGCGATTGGTGATGGATTACTCGGCAGGGTGCTTGA
TGGGCTCGGCCAGCCACTCGATTTGTTCGGCGATCTCACCGATGTTTCACAGGTTCAGCAATCACATACCAGCTATAACC
CATTATTACGACAACCTATCAGGCAACCGCTTGACGTTGGGATCCGTGCCATCAATAGCTTACTGCCTATAGGTAGAGGG
CAACGACTGGGGTTATTTGCGGGCTCTGGTGTAGGTAAAAGTGTATTACTCGGCATGATGACGCGCTTTACCGATGCGGA
TGTTGTGGTGGTGGGTTTAATTGGTGAGCGTGGCCGTGAGGTGCGTGAGTTTATTGAGGAAACGCTCGGCGAAGAGGGTC
GTAAGCGCGCGGTGATTATTGCAGCACCTGCAGATACTACCCCACTCATGCGCTTGCGTGCTATGCGCTTATCCCATTCT
ATTGCCTGTGCCTTTCGGGATCAGGGCAAGCAAGTGTTGTTATTGGTTGATTCATTGACGCGTTATGCACAGGCACAGCG
TGAAATCGCCCTGAGTTTAGGCGAGCCGCCAGCAACAAAAGGTTATCCTCCATCGGCGTTTGCACAGTTACCCAGATTAG
TTGAAATGGCGGGAAATGGTGAGCATCCAACGGGCACTCTGACGGCTTTTTATACCGTTCTAGTGGAAGGGGATGATCAG
CAGGATCCGGTTGCTGATGCTGCACGGGCTATTTTAGATGGTCATATCGTACTGAGCCGTAAACTTGCAGAACAAGGGCA
TTTTCCTGCCATTGATATTAATGCCTCGGTTAGCCGTCTTGCGATGCAGATAGCTACGGCGAGCAGTATCGAGCAGAGTC
AGATATTTAGGCATATTCTAAGCCGTTATAATGAAGTGAGAGATTTATTGCCTTTAGGAGGGTATCAAGCGGGCCAAGAT
CCTGAAATGGATCTGGCAGTACGTGTATACCCAGAGATGGCAAAATTTTTACGTCAAACCACGCAAACTAAAATCAATCT
CGCCGAGAGTTTAGTGCAGTTAGAGCAATTGATGGGAGTCATGGGATATGAGCCAGCGTCATCTCTCTAA

Protein sequence :
MRPILEADFFQDDWPRVPVARVYGRLIRVNGLLLEAVGCQLGMGDRCQIEGRHGRLIEAEVVGFHRDVLCLMPIEPTQGL
MPGARVLPITHEQTIAIGDGLLGRVLDGLGQPLDLFGDLTDVSQVQQSHTSYNPLLRQPIRQPLDVGIRAINSLLPIGRG
QRLGLFAGSGVGKSVLLGMMTRFTDADVVVVGLIGERGREVREFIEETLGEEGRKRAVIIAAPADTTPLMRLRAMRLSHS
IACAFRDQGKQVLLLVDSLTRYAQAQREIALSLGEPPATKGYPPSAFAQLPRLVEMAGNGEHPTGTLTAFYTVLVEGDDQ
QDPVADAARAILDGHIVLSRKLAEQGHFPAIDINASVSRLAMQIATASSIEQSQIFRHILSRYNEVRDLLPLGGYQAGQD
PEMDLAVRVYPEMAKFLRQTTQTKINLAESLVQLEQLMGVMGYEPASSL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 5e-71 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-71 50
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 5e-71 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-70 50
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 5e-71 50
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 5e-78 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-76 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 4e-67 45
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-75 45
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-75 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 7e-65 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 5e-66 45
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 5e-66 45
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-74 45
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-74 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 8e-67 45
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 4e-75 44
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-75 44
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-75 44
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-74 44
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-75 44
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 3e-69 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-69 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-68 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 1e-63 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG1254 Protein 8e-111 60
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG2331 Protein 5e-99 56
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG2499 Protein 3e-103 55
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG0515 Protein 3e-71 50
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG0192 Protein 8e-74 46
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG2144 Protein 4e-74 45
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG0390 Protein 1e-72 45
Sputcn32_3473 YP_001184982.1 FliI/YscN family ATPase VFG0048 Protein 8e-76 44