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Name : lysS (Sputcn32_3049) Accession : YP_001184561.1 Strain : Shewanella putrefaciens CN-32 Genome accession: NC_009438 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 3532061 - 3533563 bp Length : 1503 bp Strand : - Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGACTGAACAAGTACAAGATGAAAACAAACTGATCGCCGAGCGTCGTGCCAAGCTAGACAGCATTCGCCCGAATTGTAG CGCTAATGCTCACCCTAATACGTTCCGTCGCACCCACAAAGCGGCAGAGTTGCAGGAAAAATACGGTCAAAACACTAAGG AAGAACTCGAAGCCTTAGGTTTTAGAACCAGCATTGCTGGTCGTATCATGGCAAAACGTGGCCCATTCCTCGTAATTCAA GACGTGTCTGGTCGTATCCAAGCTTACGCTGAAAAGGGCGTTCAAGCGGATCTGAAGGATCGTTACCAAGGTCTGGATAT CGGTGACATCATCGGCGTAACTGGTCAGTTGCACTTGTCTGGTAAAGGCGATCTGTACGTCAATATGGAAGAGTACCAAC TGCTGACCAAAGCACTGCGTCCACTGCCAGAGAAATTCCACGGCTTAACCGACCAAGAAACCCGTTACCGCCAACGTTAT GTTGACTTAATCGTTAACGAAGAATCTCGTCAAGCCTTCGTGATGCGCTCAAAAGTGGTTGCAGCTATCCGTAACTTCAT GATCAAAAAAGAGTTCATGGAAGTTGAAACCCCAATGATGCACGTGATCCCAGGCGGTGCTTCAGCCCGTCCATTTATCA CGCACCACAATGCGCTGGACATGCCAATGTACCTGCGTATCGCGCCAGAGCTATACCTCAAGCGTTTAGTGGTCGGTGGT TTTGAGCGTGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTGTCGCCACGCCATAACCCAGAATTCACTATGAT GGAATTCTATATGGCTTACGCTGATTACAAAGATCTGATGGACTTAACCGAAGAACTGTTAAGCTCAATCGCGATTGAAT TGTTAGGCAGTGCGCAAATGCCATACGGCGAGCACACCGTTGATTTCGGCGGCCCATATGCCCGTCTGAGCATGTTAGAA GCGATTCAGAAGTACAATCCTGATAACGCGACTATCCAAGCCATGACCTACGAGCAAGTGAAAGATCTTGAATTTATGCG TGAATTGGCTATCAGCCTAGGTATCAAGATCGAGAAGTTCTGGACCTGTGGTCAGCTATTAGAAGAAATCTTCGGTGAAA CTGCTGAATGGCAATTAATGCAACCGACCTTCATCACTGGCTACCCAGCGGACATTTCGCCACTGGCACGTCGTAACGAT GACAATCACTTCATCACCGACCGTTTTGAATTCTTCATCGGTGGTCGTGAAGTAGCTAACGGTTTCAGCGAGTTAAATGA TGCTGAAGATCAAGACAGCCGCTTTAAAGCGCAGGTTGATGCCAAAGACGCGGGTGACGATGAAGCCATGTTCTACGATG CGGACTATATCACTGCACTGGAACACGGTTTACCACCAACAGCGGGTCAAGGTATTGGTATCGACCGTTTAGTGATGCTA TTCACTAACACTCATACAATCCGTGATGTGATCCTATTCCCTGCAATGCGCCCACAAGCCTAA Protein sequence : MTEQVQDENKLIAERRAKLDSIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFRTSIAGRIMAKRGPFLVIQ DVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY VDLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLE AIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRND DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML FTNTHTIRDVILFPAMRPQA |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 5e-130 | 57 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001184561.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 3e-130 | 57 |
