Gene Information

Name : leuS (Sputcn32_2863)
Accession : YP_001184381.1
Strain : Shewanella putrefaciens CN-32
Genome accession: NC_009438
Putative virulence/resistance : Unknown
Product : leucyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG0495
EC number : 6.1.1.4
Position : 3324659 - 3327238 bp
Length : 2580 bp
Strand : +
Note : leucine--tRNA ligase; LeuRS; class-I aminoacyl-tRNA synthetase; charges leucine by linking carboxyl group to alpha-phosphate of ATP and then transfers aminoacyl-adenylate to its tRNA; due to the large number of codons that tRNA(Leu) recognizes, the leucyl

DNA sequence :
ATGCAAGAGCAATATAATCCCTCAGAAATTGAAGCCTTAGTGCAAAAGCACTGGCATGACAACAAAACCTTCGAAGTCAC
TGAAGACGCAAACAAAGAAAAGTTTTATTGCCTTTCGATGTTTCCCTATCCTTCTGGCCGACTCCACATGGGACACGTTC
GCAACTATACCATAGGTGACGTCGTTGCGCGCTTCCAGCGTCTTCAAGGCAAAAATGTGCTGCAACCAATTGGTTGGGAC
TCCTTTGGTTTGCCTGCAGAAAATGCCGCGATTAATAATAAAACAGCACCTGCGCCATGGACCTACGAAAACATAGAGTA
CATGAAAAACCAGCTAAAACTGCTAGGTTTTGGTTACGATTGGAGCCGTGAAATCGCCACTTGTACCCCAGAATATTATC
GCTGGGAACAATGGTTCTTCACTAAGTTGTACGAGAAGGGCCTAGTCTATAAAAAGACTGCTTCCGTCAACTGGTGTCCA
AACGATGAAACCGTGCTGGCGAACGAGCAAGTACAAGACGGTTGCTGCTGGCGCTGTGATACCCCTGTGGAACAAAAAGA
AATTCCACAGTGGTTTATTAAGATCACTGCCTATGCAGAAGAATTATTAAACGATATCGATACCTTAGATGGCTGGCCAG
AACAGGTCAAAACCATGCAGCGTAACTGGATTGGTCGCAGCGAAGGTGTAGAAATGACCTTCGGCGTTGCAGGTCACGAT
AAAACCTTCGATATCTACACCACGCGTCCAGATACCTTAATGGGCGTGACCTATGTGGCGATTGCCGCAGGTCATCCATT
GGCAGAAATCGCCGCGCAAACGAATCCTGAGCTTGCCGCCTTTATCGATGAGTGCAAAAACAGCACAACATCTGAAGCCG
AACTCGCGACTATGGAAAAACGCGGTGTCGCTACAGGACTTTTCGCCATTCATCCCATCACTGGCAAGCAAGTGCCGATT
TGGGCCGCTAACTTTGTGCTGATGAACTATGGCACAGGTGCCGTAATGTCAGTTCCTGGGCACGACCAACGTGACTTTGA
ATTTGCAAAAAAATATGGCCTGGCGATTGAAGCTGTGATCAAGCCAGTTGATGGCGACGTTGATATCAGTGAAGCCGCTT
ACACCGAAAAAGGCATTCTGTTTAACTCGGGCGAGTTCGACGGTTTAGACTTTGAAGCGGGCTTTAATGCCATTGCTAAC
AAGTTAGTCGCCGAAGGTAAAGGCAAGCGCCAAGTCAACTATCGCCTGCGCGATTGGGGTGTTTCTCGTCAGCGTTACTG
GGGCGCGCCTATTCCTATGGTGACCTTAGCTGACGGCACTGTGATCCCAACGCCAGCAGATCAACTGCCAGTATTGCTGC
CAGAAGATGTCGTGATGGACGGGATTCAAAGCCCAATCAAAGCCGATAAAGAATGGGCCAAAACCCAAGTGAACGGTCAA
GATGCACTGCGCGAAACCGATACCTTCGATACCTTTATGGAATCCTCTTGGTATTATGCCCGTTACTGTAGCCCGCAGGC
TGACGAAATGTTAGATCCCGCTAAAGCCAACTACTGGCTACCCGTAGATCAATACATTGGTGGTATCGAACACGCTTGTA
TGCATTTGTTATATTTCCGCTTCTTCCACAAGTTGCTGCGTGATGCGGGTCTGGTTAACTCTAACGAGCCAGCCAAACAA
TTGCTGACTCAGGGTATGGTGTTGGCTGATGCTTTCTACTACATCAACGAAAAAGGCGCCCGTGTGTGGGTTTCTCCTTT
AGATGTAGCGACCACCGAAAAAGACGACAAGGGCCGTATCACTAAGGCTATAGACAAAGACGGCAACGAACTGGTTTACA
CTGGTATGTGCAAGATGTCTAAGTCGAAAAACAACGGTATCGACCCACAGGTGATGGTTGAAAAATATGGCGCAGACACT
GTGCGTCTATTTATGATGTTCGCCTCACCGCCAGAATTAACCCTGGAATGGCAAGAGTCTGGCGTTGAAGGCGCACACCG
CTTCATTAAACGTCTGTGGAAGCTTGCAAGCGAGTATATCGCCCAAGATAACAGTGAAGCCTTAGATGTCAGCAAGTTAA
CAAGCGAGCAAAAAGCGCTGCGCCGTGAAGTCCACAAGACCATTGCCAAAGTGACCGACGATATCGGCCGCCGTCAAATG
TTCAACACTGCGGTTGCCGCCGTGATGGAACTGATGAACCACCTGCAAAAAGCGCCACAAACCACAGGCCAAGACAGAGC
CATTATCGGTGAAGCCTTATCTGCCGTCATGCGTTTACTGTACCCCATTATTCCACATGTGAGCTTTACCTTGTGGAATG
ACTTAGGTAATACAGGCAGCATCGAAGACAGCCAATGGCCGGTTGTTGATGAATCTGCCTTGGTAGAAGACAGCAAACTG
ATCGTGGTACAAGTGAACGGTAAAGTTCGCGCTAAAATCACTGTTGCCGCCGATGCGGATAAAGACTCAGTTGAAGCCTT
GGGTATGAATGATGAGCATGTGATCAAATACCTAGATGGCCTGACAGTACGTAAAGTGATCTATGTACCAGGTAAACTGC
TCAGCATAGTGGCAAATTAA

Protein sequence :
MQEQYNPSEIEALVQKHWHDNKTFEVTEDANKEKFYCLSMFPYPSGRLHMGHVRNYTIGDVVARFQRLQGKNVLQPIGWD
SFGLPAENAAINNKTAPAPWTYENIEYMKNQLKLLGFGYDWSREIATCTPEYYRWEQWFFTKLYEKGLVYKKTASVNWCP
NDETVLANEQVQDGCCWRCDTPVEQKEIPQWFIKITAYAEELLNDIDTLDGWPEQVKTMQRNWIGRSEGVEMTFGVAGHD
KTFDIYTTRPDTLMGVTYVAIAAGHPLAEIAAQTNPELAAFIDECKNSTTSEAELATMEKRGVATGLFAIHPITGKQVPI
WAANFVLMNYGTGAVMSVPGHDQRDFEFAKKYGLAIEAVIKPVDGDVDISEAAYTEKGILFNSGEFDGLDFEAGFNAIAN
KLVAEGKGKRQVNYRLRDWGVSRQRYWGAPIPMVTLADGTVIPTPADQLPVLLPEDVVMDGIQSPIKADKEWAKTQVNGQ
DALRETDTFDTFMESSWYYARYCSPQADEMLDPAKANYWLPVDQYIGGIEHACMHLLYFRFFHKLLRDAGLVNSNEPAKQ
LLTQGMVLADAFYYINEKGARVWVSPLDVATTEKDDKGRITKAIDKDGNELVYTGMCKMSKSKNNGIDPQVMVEKYGADT
VRLFMMFASPPELTLEWQESGVEGAHRFIKRLWKLASEYIAQDNSEALDVSKLTSEQKALRREVHKTIAKVTDDIGRRQM
FNTAVAAVMELMNHLQKAPQTTGQDRAIIGEALSAVMRLLYPIIPHVSFTLWNDLGNTGSIEDSQWPVVDESALVEDSKL
IVVQVNGKVRAKITVAADADKDSVEALGMNDEHVIKYLDGLTVRKVIYVPGKLLSIVAN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
leuS YP_281511.2 leucyl-tRNA synthetase Not tested Not named Protein 1e-160 43