Gene Information

Name : Csac_1372 (Csac_1372)
Accession : YP_001180165.1
Strain : Caldicellulosiruptor saccharolyticus DSM 8903
Genome accession: NC_009437
Putative virulence/resistance : Resistance
Product : D-alanyl-alanine synthetase A
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : 6.3.2.4
Position : 1543671 - 1544765 bp
Length : 1095 bp
Strand : +
Note : TIGRFAM: D-alanine--D-alanine ligase; PFAM: D-alanine--D-alanine ligase domain protein; RimK domain protein ATP-grasp

DNA sequence :
ATGACCAAATTAAAGGTTGCAGTTTTGTTCGGTGGAGTTTCAACAGAGCATGAAGTTTCGATAGTATCTGCAAAATCGGT
TATGCAAAACCTTGACAAGGAAAAATACGAAATAATACCAATTGGTATTACAAAAGAGGGAAAATGGCTTTTGTACACAG
GCAAGATAGAAGATTTAGACAGCAAATGGACAATGTATTCAATTGAATGTTTTATCTCACCAGACAGGACTAAAAAAGCG
CTTGTGAAGATAAAAGATAGTGAAGCTACCTTTATTGACATTGACGTTGTATTTCCTGTATTGCATGGATTAAATGGTGA
GGATGGTACTGTACAAGGCCTTTTAGAACTCTCTGGTATTCCATATGTTGGGTGTGGAGTGTTGTCATCAGCGCTATGCA
TGGACAAAGCTTTCGCCAAAAAACTTGCTCTTTTAGAGGGGATTCCACAGGGCCACTTTTTGGTGATTTACAAAGATGAG
TATCTTTCCAAAAAAGAATATTTCATCAGACGGATTGAAAGTGAGTTTTCGTATCCTGTATTTGTAAAGCCTGCAAATTC
GGGCTCCTCAGTTGGGATTTCAAAGGCAAAAGACAAAGACGAGCTGATTTTGGCAATCCACGAGGCATTTTTGTATGATA
CCAAGATTTTGATCGAACAGGCTATAAATGCACGTGAGATTGAGTGTGCAGTGCTTGGCAATAGCGAAATTCTCGTATCG
GCCCTGGGTGAGATAGTACCATCGCGAGAGTTTTATTCATATGAAGCCAAGTATATAGATGGCTCATCAGAATTGATCAT
TCCTGCGAAACTTGAAAAGCAGGTAGAGGATGAGATTAAGGAGCTTGCTGTTAAGATTTACAAGCTTTTTGAGTGTAGTG
GTATGGCAAGAATAGACTTTTTTGTTGACAAAGAAACTAACAAGGTGTATTTCAATGAAGTAAATACAATCCCTGGCTTT
ACAAGTATTTCTATGTATCCAAAATTGATGGAGTACAGCGGTATACCATATCCTCAACTTCTTGACAAACTCATACAACT
TGCAATTGAAAAGAACCAACAAAAACAAAGCATAAAATATAGCAAAGAGGGTTAA

Protein sequence :
MTKLKVAVLFGGVSTEHEVSIVSAKSVMQNLDKEKYEIIPIGITKEGKWLLYTGKIEDLDSKWTMYSIECFISPDRTKKA
LVKIKDSEATFIDIDVVFPVLHGLNGEDGTVQGLLELSGIPYVGCGVLSSALCMDKAFAKKLALLEGIPQGHFLVIYKDE
YLSKKEYFIRRIESEFSYPVFVKPANSGSSVGISKAKDKDELILAIHEAFLYDTKILIEQAINAREIECAVLGNSEILVS
ALGEIVPSREFYSYEAKYIDGSSELIIPAKLEKQVEDEIKELAVKIYKLFECSGMARIDFFVDKETNKVYFNEVNTIPGF
TSISMYPKLMEYSGIPYPQLLDKLIQLAIEKNQQKQSIKYSKEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 5e-59 43
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 7e-60 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A AY489045.gene.p01 Protein 3e-58 45
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A AF277571.gene.p01 Protein 1e-59 45
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A NC_005054.2598280.p0 Protein 2e-62 44
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A NC_014475.1.orf3.gen Protein 2e-62 44
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A AF130997.1.orf4.gene Protein 2e-59 44
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A AY145441.gene.p01 Protein 7e-60 43
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A U35369.1.gene6.p01 Protein 3e-60 43
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A AE016830.1.gene2250. Protein 3e-60 43
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A AF310956.2.orf5.gene Protein 7e-60 43
Csac_1372 YP_001180165.1 D-alanyl-alanine synthetase A U00456.gene.p01 Protein 3e-60 43