Gene Information

Name : lysS (Ent638_3308)
Accession : YP_001178023.1
Strain : Enterobacter sp. 638
Genome accession: NC_009436
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3611754 - 3613271 bp
Length : 1518 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAACAACAAGCACAGGGCACTGACGCGGTAGTCGATCTTAATAACGAGCTGAAAACACGTCGCGAAAAGCTGGC
TGCACTGCGTGAGCAGGGTATTCCATTCCCGAACGATTTTCGTCGCGATCACACTTCCGATCAGCTGCATGCTGACTTCG
ATGCAAAAGAAAACGAAGAGCTGGAAGCGCTGAACGTAGAAGTGTCCGTTGCAGGCCGCATGATGACCCGCCGTATCATG
GGTAAAGCGTCCTTCGTGACGTTGCAGGACGTCGGTGGTCGTATTCAGCTGTACGTTTCCCGCGACGATCTGCCAGAAGG
CATTTATAACGAGCAGTTCAAAAAGTGGGACCTGGGCGACATCCTTGGCGCGAAAGGCAAGCTGTTCAAGACCAAGACGG
GTGAACTCTCTATTCACTGCACCGAACTGCGCCTGCTGACCAAAGCGCTGCGTCCGTTGCCGGATAAATTCCACGGTCTG
CAGGATCAGGAAGCGCGCTATCGCCAGCGCTACCTGGATCTCATCTCTAACGATGAATCCCGCAAAACGTTCAAAATCCG
TTCCCAGATTATGGCGGGCATCCGTCAGTTCATGGTTGGCCGTGATTTTATGGAAGTCGAAACGCCAATGATGCAGGTGA
TTCCGGGTGGTGCTTCTGCGCGTCCGTTCGTCACCCATCACAATGCGCTGGATCTCGACATGTACCTGCGCATCGCGCCG
GAACTGTACCTGAAGCGTCTGGTGGTTGGTGGCTTCGAACGCGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGTAT
TTCCGTTCGCCATAACCCAGAGTTCACCATGATGGAACTCTATATGGCGTACGCGGATTACAAAGATCTGATCGAGCTGA
CCGAGTCTCTGTTCCGCACCCTGGCGCAGAATATTCTGGGCACCACGGAAGTGCCTTACGGCGACGAAGTGTTCGACTTT
GGTAAGCCGTTCGTGAAGCTGACCATGCGCGAAGCGATCAAGAAATACCGTCCAGAAACCGAAATGGCCGATCTGGATAA
CTACGATTCTGCGAAAGCCATCGCGGAAAGCATCGGTATCAAAGTTGAGAAGAGCTGGGGTCAGGGTCGTATCGTGACTG
AGATCTTCGAAGAAGTGGCAGAAGCGCATCTGATTCAGCCAACCTTCATCACCGAATACCCTGCTGAAGTGTCTCCACTG
GCGCGTCGTAATGACGAAAACCCGGAAATCACTGACCGCTTTGAGTTCTTCATCGGTGGCCGCGAAATCGGTAACGGCTT
TAGCGAGCTGAACGATGCGCAGGATCAGGCGCAGCGCTTCCAGGATCAGGTTGATGCGAAAGCAGCAGGCGACGATGAAG
CGATGTTCTTCGACGAAGACTACGTGACTGCCCTGGAACACGGCTTGCCGCCAACGGCGGGTCTGGGTATTGGTATCGAC
CGTATGGTGATGCTGTTTACCAATAGCCACACCATCCGTGATGTGATCCTGTTCCCGGCGATGCGTCCGGTAAAATAA

Protein sequence :
MSEQQAQGTDAVVDLNNELKTRREKLAALREQGIPFPNDFRRDHTSDQLHADFDAKENEELEALNVEVSVAGRMMTRRIM
GKASFVTLQDVGGRIQLYVSRDDLPEGIYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
QDQEARYRQRYLDLISNDESRKTFKIRSQIMAGIRQFMVGRDFMEVETPMMQVIPGGASARPFVTHHNALDLDMYLRIAP
ELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMELYMAYADYKDLIELTESLFRTLAQNILGTTEVPYGDEVFDF
GKPFVKLTMREAIKKYRPETEMADLDNYDSAKAIAESIGIKVEKSWGQGRIVTEIFEEVAEAHLIQPTFITEYPAEVSPL
ARRNDENPEITDRFEFFIGGREIGNGFSELNDAQDQAQRFQDQVDAKAAGDDEAMFFDEDYVTALEHGLPPTAGLGIGID
RMVMLFTNSHTIRDVILFPAMRPVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 2e-159 69
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 7e-87 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001178023.1 lysyl-tRNA synthetase VFG1668 Protein 1e-159 69