Gene Information

Name : entE (Ent638_1126)
Accession : YP_001175858.1
Strain : Enterobacter sp. 638
Genome accession: NC_009436
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 1238743 - 1240353 bp
Length : 1611 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence : 
ATGACTCTTCCCTTTACCCGTTGGCCTGAGGCATTTGCCCGGCGCTACCGTGAAAAAGGCTACTGGCAGGATGTGCCGCT
GACCGACATTTTGACGCGTCATACTGACAACGACGCAACGGCGGTGATCGACGGTGAACGGCACTACACCTACCGTCAGC
TGCACCAGGCAGCGAATAATCTTGCGTCCGCACTGCAGGCGCAGGGGATCAAGCGCGGCGAAACGGCGCTGGTGCAGTTG
GGCAACGTGGCCGAGTTTTACATCACTTTCTTTGCCCTGCTGCAGGCGGGCATTGCCCCCGTGAATGCGCTCTTTAGCCA
TCAACGCAGCGAGCTGAACGCGTACGCACTTCAAATTACGCCTGCGCTGGTGATTGCCGATCGCCAGCATGCGCTGTTTG
CGGGCGATGATTTCCTGAATACGTTTGTCGATGAACATCGCTCTGTGCGTGTGGTGCTGCTGCGCGGCGATAACGGTGAA
CACGCGCTGGAGAAGGCCATCGCGCGTCCGGCTGACAACTTTATCGCCAGCCCAACGCCTGCCGATGAAGTGGCGTTTTT
TCAGCTTTCCGGCGGGAGCACCGGCACGCCAAAACTGATTCCCCGCACGCACAACGACTACTACTACAGCATTTTGCGCA
GCAACGAGATTTGCGGGATTACGGCAGACACTCGCTACCTGAACGCGCTGCCCGCGGCGCACAACTTTGCGATGAGTTCC
CCAGGATCGCTGGGCGTGTTTATGGCCGGTGGGTGCGTGGCGCTGGCAAACGATCCGAGCGCCACGATCTGCTTCCCGCT
GATCGAAAAACACCAGCTCAACGTTGCGTCTTTGGTTCCTCCAGCGGTCAGCCTGTGGTTGCAGGCCATTGCAGAAGGGG
CGGGTAACGCTCAACTGGCATCACTGACGCTGTTACAGGTGGGCGGAGCACGGCTTTCCGCCACGCTTGCGGCGCGCATT
CCGGCGGAAATCGGCTGTCAGCTGCAGCAGGTTTTTGGCATGGCGGAAGGGCTGGTGAACTACACCGCGCTCGACGACAC
CCCGGAACGCATCATTAATACTCAGGGCCGTCCGATGTGTCCGGACGACGAAGTGTGGGTGGCGGATGAAAACGGACAAC
CGCTGCCGCGCGGTGAAGTCGGGCGTTTGATGACGCGCGGGCCGTACACCTTCCGTGGCTATTTCAACAGCCCGGAACAC
AACGCCAGCGCTTTTGATGCCGACGGTTTTTACTGCTCCGGGGATCTGATCGCCATTGACGAGCAGGGTTACATCACCGT
ACAGGGGCGCGAAAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCGGCGGAAGAGATCGAGAATTTACTGCTGCGTC
ACGAATCGGTGATCCACGCGGCGCTGGTCAGCATGGAAGACAGCCTGCTGGGCGAAAAAAGCTGCGCGTATCTGGTGGTG
AAAAAGCCCCTGCGCGCGGTGGATGTGCGTCGATTCCTGCGCGAGCAGGGGATTGCAGAATTCAAACTGCCGGACAGGGT
CGAGTGCGTGGACGCGCTGCCGCTGACGCCGGTCGGGAAAGTAGACAAGAAACAATTGCGCTTGTGGCTCGCTGAACGCG
CCCAGGGCTGA

Protein sequence : 
MTLPFTRWPEAFARRYREKGYWQDVPLTDILTRHTDNDATAVIDGERHYTYRQLHQAANNLASALQAQGIKRGETALVQL
GNVAEFYITFFALLQAGIAPVNALFSHQRSELNAYALQITPALVIADRQHALFAGDDFLNTFVDEHRSVRVVLLRGDNGE
HALEKAIARPADNFIASPTPADEVAFFQLSGGSTGTPKLIPRTHNDYYYSILRSNEICGITADTRYLNALPAAHNFAMSS
PGSLGVFMAGGCVALANDPSATICFPLIEKHQLNVASLVPPAVSLWLQAIAEGAGNAQLASLTLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTALDDTPERIINTQGRPMCPDDEVWVADENGQPLPRGEVGRLMTRGPYTFRGYFNSPEH
NASAFDADGFYCSGDLIAIDEQGYITVQGREKDQINRGGEKIAAEEIENLLLRHESVIHAALVSMEDSLLGEKSCAYLVV
KKPLRAVDVRRFLREQGIAEFKLPDRVECVDALPLTPVGKVDKKQLRLWLAERAQG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-82 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-82 42
ybtE CAA21394.1 - Virulence HPI Protein 5e-82 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 9e-82 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-82 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-81 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-82 42
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-82 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 5e-82 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_001175858.1 enterobactin synthase subunit E VFG0932 Protein 0.0 81
entE YP_001175858.1 enterobactin synthase subunit E VFG0168 Protein 1e-105 45