Gene Information

Name : cadA-2 (PST_0664)
Accession : YP_001171212.1
Strain : Pseudomonas stutzeri A1501
Genome accession: NC_009434
Putative virulence/resistance : Resistance
Product : cadmium translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 763480 - 765762 bp
Length : 2283 bp
Strand : +
Note : Code: P; COG: COG2217

DNA sequence :
ATGGACTGCCCTACCGAGCAGACGCTGATTCAGGACAAACTGAGCAAGCTCGCAGGCATAGATAAGCTCGACTTCAACCT
GATCAATCGGATTCTTGGGGTATGGCACAGCTTGCCGTCGACCGCGCCTATCGAAGCGGCGATCTCGTCCCTGGGAATGC
AGGCAGAACCGCTATCTGCTGAAGGGCAAGCCCGCATCAAAATCGAACAGATGGACTGCCCAACTGAAGAAAAGCTGATC
CGCGACAAGCTGACCTCGCTACCCGGTATCAGTGCATTGGAGTTCAACCTGGTTCAACGTGTACTGACGCTCAGCCACAC
AGCAGAAGCGCTGCCAGCAGCACTGGCAGCGATTCGCGAGCTTGGTTTCACGCCAGTGGTTGAGGGTTCAGCAACAGCAC
GTGAAGAAGAGCAAGGCACCCCTCACAAGAAAGCCTGGTGGCCCTTGGCGCTCTCGGGGGTGGCCGCCTTATCGGCGGAA
GTGATCCACTTTGCCGAGCTTGCGCCAGATTGGGCTGTGGCTGGCGTGGCGCTGCTCGCCATTCTGCTGTGCGGCCTGAC
GACCTATAAAAAGGGCTGGATCGCGCTGAAGAACCGCAACCTGAATATCAATGCGTTGATGAGTATCGCTGTGACCGGTG
CGGTGCTGATTGGGCAATGGCCAGAAGCGGCCATGGTCATGTTCCTCTTTTCTGTCGCCGAGTTGATCGAAGCGAAGTCG
CTGGATCGGGCTCGCAATGCCATCCGTGGCCTGATGGATCTGACGCCAGAGCGCGCCACGGTGCAGCAGGTCGATGGCAC
ATGGCTGGAAGTTGACGTTAAAACCATCGCCGTAGGCGCATTGGTCAGGGTTCGACCGGGCGAACGAATCGGTCTCGATG
GCGAAGTTACTGCCGGCAACTCCACCATCAACCAAGCGCCCATCACGGGGGAAAGCCTGCCCGTAGAGAAAACCATCGGC
GATCCGGTTTTTGCGGGAACCATCAATGAAGCCGGCTCCCTGGAATACCGAGTCCTGGCCGCCGCAGCCAACACCACGCT
GGCACGTATCATTCACGCGGTCGAAGAGGCGCAGGGTTCACGCGCGCCGACCCAGCGCTTTGTCGATCAATTCGCCAAGG
TATACACCCCTGTCGTATTCCTATTCGCCCTGGCTATCGCCATTGTGCCGCCACTCTTCATGGCCGAGCCCTGGTACGAC
TGGATCTACCGTGCCCTGGTACTGCTGGTGGTTGCCTGCCCTTGCGCCTTGGTCATCTCGACACCTGTCACCATTGTCAG
TGGTTTAGCTGCGGCTGCACGCAAAGGCATCCTGATCAAAGGCGGCGTTTATCTGGAAACTGGCGGCAAGCTTGGCTTCC
TGGCCCTCGACAAGACCGGAACCATTACCCATGGCAAACCCGTGCAAACCGATTACATGCCACTGGTTGAACAGGACTCA
GAACTCTGTCGCACTCATGCTGCCAGCCTCGCCAGTCGCTCTGATCACCCGGTTTCGCAAGCGATAGCCAAGCACGCCAC
TGAGAATGGCCTGATGTTCGCTACCGTTGAGGGATTCGAAGCCCTGCCGGGTCGAGGTGTACGGGGCACCATTGAAGGCA
TGGATTTCCACCTGGGTAACCACCGTCTGGTGGAAGAGCTTGGCCTCTGCTCACCCGAGCTGGAAACGACGCTGGAACGT
CTCGAACGCCAAGGCAAAACAGTGGTGGTGTTGTGCAATCCGCAGCGTGCCATTGCCCTGTTCGCCGTTGCCGATACCGT
CAAGGACTCCAGCCGCCAGGCAATCGAGGAGCTTCATGCGCTGGGTGTGAAGACCACGATGCTGACTGGCGATAACCCGC
ACACCGCAGAAGCCATCGCACAGCAGGTGGGCATCGACGAAGCACGCGGCAATCTCCTGCCAGTCGACAAATTGCAGGCC
GTTGAAGCATTGCAGGCGCGCGGCTACGTTGTTGGGATGGTGGGCGACGGCATCAACGATGCGCCTGCGCTGGCGAAGTC
CGAAATCGGCTTCGCCATGGCCGCCGCCGGCACGGACACGGCCATCGAAACTGCGGATGTGGCCTTGATGGATGACGACC
TGCGCAAGATCCCGGCCTTCATCCGCCTATCCCAACAGACCGCCGTCATCCTCAAGCAGAACATCGTGCTGGCGTTAGGG
ATCAAGGCCCTGTTTCTCGCCATTACCCTAACGGGGCAGGCGACGATGTGGATGGCCGTCTTTGCCGACATGGGCGTCAG
CCTGCTGGTGGTGTTCAACGGCCTGCGCCTGCTTAAAAAGTAG

Protein sequence :
MDCPTEQTLIQDKLSKLAGIDKLDFNLINRILGVWHSLPSTAPIEAAISSLGMQAEPLSAEGQARIKIEQMDCPTEEKLI
RDKLTSLPGISALEFNLVQRVLTLSHTAEALPAALAAIRELGFTPVVEGSATAREEEQGTPHKKAWWPLALSGVAALSAE
VIHFAELAPDWAVAGVALLAILLCGLTTYKKGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVMFLFSVAELIEAKS
LDRARNAIRGLMDLTPERATVQQVDGTWLEVDVKTIAVGALVRVRPGERIGLDGEVTAGNSTINQAPITGESLPVEKTIG
DPVFAGTINEAGSLEYRVLAAAANTTLARIIHAVEEAQGSRAPTQRFVDQFAKVYTPVVFLFALAIAIVPPLFMAEPWYD
WIYRALVLLVVACPCALVISTPVTIVSGLAAAARKGILIKGGVYLETGGKLGFLALDKTGTITHGKPVQTDYMPLVEQDS
ELCRTHAASLASRSDHPVSQAIAKHATENGLMFATVEGFEALPGRGVRGTIEGMDFHLGNHRLVEELGLCSPELETTLER
LERQGKTVVVLCNPQRAIALFAVADTVKDSSRQAIEELHALGVKTTMLTGDNPHTAEAIAQQVGIDEARGNLLPVDKLQA
VEALQARGYVVGMVGDGINDAPALAKSEIGFAMAAAGTDTAIETADVALMDDDLRKIPAFIRLSQQTAVILKQNIVLALG
IKALFLAITLTGQATMWMAVFADMGVSLLVVFNGLRLLKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 66
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 6e-115 48
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 5e-115 48
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 5e-115 47

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA-2 YP_001171212.1 cadmium translocating P-type ATPase BAC0298 Protein 0.0 67
cadA-2 YP_001171212.1 cadmium translocating P-type ATPase BAC0461 Protein 2e-73 41