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Name : lysS (Pnuc_1472) Accession : YP_001156250.1 Strain : Polynucleobacter necessarius QLW-P1DMWA-1 Genome accession: NC_009379 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 1550254 - 1551798 bp Length : 1545 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGAACGATAAAACGAATCCAGCCCCAGCTACCGAAGTAGTTGATGAAAATCACATCATTGCAGAGCGTCGTGAAAAACT CGCTAAGCTTCGTGAGAATGGCATCGCCTTTCCAAATGATTTTGTACCAACCCATTTGGCCGTTGATTTACACGCGCACT ATGACAGCCTCACTAAAGAAGAGCTTGCTGCCAAGAAAATTCACGTCAAAGTAGCCGGCCGCATGGTGCTTAAGCGCGTC ATGGGTAAAGCCAGCTTTGCAACGATTCAAGATCGCAGCGGACAAATCCAGTTTTATATTAATGATGAAATTAGCGGCGC TGATACCCACGGGGCTTTTAAGCACTGGGATATGGGTGATTTTATTTCGGCTGAAGGCAATCTCTTTAAGACCAATAAAG GTGAATTATCAGTAGAGTGCAGCAATTTACGCCTGCTCAGCAAGTCCTTGCGCCCATTGCCAGATAAGTTCCACGGCCTC TCAGACCTAGAGACTAAGTATCGCCAGCGCTATGTGGATTTAATTGTGAATCCAGAAAGTCGCAATACTTTTAAAGCACG TAGTAATGCGATCGCTTCATTGCGTCGCCATATGCTTGATGCTGACTTCATGGAAGTCGAAACGCCAATGCTCCACCCTA TTCCTGGTGGCGCCACTGCAAAACCATTTATCACGCACCACAATGCGCTCGACATGCAAATGTTCTTGCGTATCGCTCCT GAGCTCTACTTAAAGCGCTTGGTAGTTGGTGGCTTTGAACGTGTCTTTGAAATTAACCGCAACTTCCGTAACGAAGGTGT GAGCCCGCGCCACAATCCAGAATTTACGATGATGGAATTCTACGCAGCCTATACCGATTACCGTTGGCTCATGGATTTCA CTGAAGGCCTCATTCGTGCAGCCGCAGTCGATGCCCAAGGCACTGCAGTTCTAACTCACCAAGGTCGTGAATTGGATCTC AGTAAACCATTCCAACGCTTGACGATTAATGAAGCCATCCTCAAGTACTGCGCTCAATCTGGTAAGAGCTATGAAGCTAT CCAGTTAGATGATGCAGACTTCATTCGCGCAGAATTGAAAAAAGGTGGTGAGAATCCAGATTCCCCCACCCTCAAGAATG CGGGTATTGGCGCTTTGCAATTGGCCTTGTTTGAGCTGGTCGCAGAATCGCATCTATGGGAACCAACCTACATCATTGAT TACCCAATTGAAGTAAGCCCGCTGGCTCGTGAATCTGATACACGCCCAGGTATTACAGAGCGTTTTGAGCTATTTATTAC TGGTCGCGAAATTGCTAATGGTTTCTCCGAGTTAAATGATGCAGAAGATCAAGCCAATCGTTTCCGCAAGCAAGTAGAGC AAAAAGAAGCGGGTGATGAGGAGGCTATGTATTTTGATCATGACTTCATTCGCGCCCTAGAATACGGTATGCCTCCGACG GGTGGTTGCGGTATTGGTATCGACCGCTTGGTCATGCTTCTCACTGATGCACCAAATATTCGTGACGTCATTCTCTTCCC ACATTTGCGTCGCGAAGAAGAATAA Protein sequence : MNDKTNPAPATEVVDENHIIAERREKLAKLRENGIAFPNDFVPTHLAVDLHAHYDSLTKEELAAKKIHVKVAGRMVLKRV MGKASFATIQDRSGQIQFYINDEISGADTHGAFKHWDMGDFISAEGNLFKTNKGELSVECSNLRLLSKSLRPLPDKFHGL SDLETKYRQRYVDLIVNPESRNTFKARSNAIASLRRHMLDADFMEVETPMLHPIPGGATAKPFITHHNALDMQMFLRIAP ELYLKRLVVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEGLIRAAAVDAQGTAVLTHQGRELDL SKPFQRLTINEAILKYCAQSGKSYEAIQLDDADFIRAELKKGGENPDSPTLKNAGIGALQLALFELVAESHLWEPTYIID YPIEVSPLARESDTRPGITERFELFITGREIANGFSELNDAEDQANRFRKQVEQKEAGDEEAMYFDHDFIRALEYGMPPT GGCGIGIDRLVMLLTDAPNIRDVILFPHLRREEE |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-126 | 55 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001156250.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 6e-127 | 55 |
