Gene Information

Name : lysS (ASA_0940)
Accession : YP_001140820.1
Strain : Aeromonas salmonicida A449
Genome accession: NC_009348
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 965417 - 966955 bp
Length : 1539 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGTCTGAACAAACCACAACTCCGGAAGTCGACCACACTCTCGAACTGAACAACGAGATGACGGAGCGTCGTTCCAAGCT
GGCCGCCTTGCGCGCCCAGGGCAACCCCTTCCCCAACGACTTCCGTCGTGACAGCCTCTCCGGCGATCTGCATGCCGAAT
TTGGCGACAAGAGCGCCGAAGAGCTGGTGGCCTTGGGTAAACAGGTGAAGATTGCCGGTCGCATCATGACCCGCCGGATC
ATGGGCAAGGCGTCTTTCGCCACCCTGCAGGACATGGCTGGCAAGATCCAGATCTACGTGACCCGTGACGATCTGCCGGA
AGGCTTCTACAACGAGCAGTTCAAGAAGTGGGATCTGGGTGACATAGTCGGCGTGGAAGGGACCCTGTTCCGTACCAACA
CAGGCGAACTCTCGGTACACGTCTCCACCATCCGCCTGCTGACCAAGGCACTGCGTCCGCTGCCCGAGAAGCACAAGGGC
CTGACCGACCAGGAAGCTCGCTGCCGCCAGCGTTACCTGGATCTGATCGCCAACGAAGAGTCCCGCAAGACCTTCATGAT
CCGCACCAAAGTGGTTGCCGGCATACGCAAGTTCTTCAACGACAAGCGCTTCATGGAAGTGGAAACCCCCATGATGCAGG
TCATCCCGGGTGGCGCCTCCGCGCGTCCGTTCGTCACTCACCACAATGCGCTGGACATCGACATGTACCTGCGTATCGCC
CCCGAGCTTTACCTGAAGCGTCTGGTGGTCGGTGGTTTCGAGCGCGTGTACGAGATCAACCGCAACTTCCGTAACGAAGG
TATCTCGGTACGTCACAACCCCGAGTTCACCATGATCGAGTTCTACATGGCCTATGCCGACTACATCGATCTGATGGACC
TGACCGAGGAGCTGCTGCGCACCCTGGCCCAGGACATTCTGGGTGACACCAAGATCCGTTACGCCAAAGAGGGCGAAGAG
GGTCTGACCATCGATTTCGGTCAGCCGTTCCAGCGCCTCACCATGGTGGACTCCATCCTGAAGTTCAACCCGGATGTGAC
CCCGGCGGATCTGGCTACCCTGGAGAGCGCCAAGGCGGTTGCCAAGCATCTGCACATCGAGCTGATGAAGGGCTGGGAGC
TGGGCCACGTGATCACCGCCATCTTCGAAGAGACGGTAGAGCACATGCTGCTGCAACCCACCTTCATCACCGAGTACCCG
GCCGCTGTTTCTCCGCTGGCGCGCCGCAACGACGTGAACCCGGACGTGACAGACCGCTTCGAATTCTTCATCGGTGGCCG
CGAGCTGGCCAACGGCTTCTCCGAGCTGAACGATGCGGAAGACCAGGCCAAGCGCTTCCAGGATCAGGTGAACCAGAAAG
AAGCCGGTGACGACGAAGCCATGTTCTACGACGCCGACTTCGTGACCGCGCTGGAACACGGCCTGCCGCCCACTGCCGGT
CAGGGTATCGGTATCGACCGTCTGGTGATGCTGTTCACCAACAGCCACACCATCCGTGACGTCATCCTGTTCCCGGCCCT
GCGCCCGCAACAGAAATAA

Protein sequence :
MSEQTTTPEVDHTLELNNEMTERRSKLAALRAQGNPFPNDFRRDSLSGDLHAEFGDKSAEELVALGKQVKIAGRIMTRRI
MGKASFATLQDMAGKIQIYVTRDDLPEGFYNEQFKKWDLGDIVGVEGTLFRTNTGELSVHVSTIRLLTKALRPLPEKHKG
LTDQEARCRQRYLDLIANEESRKTFMIRTKVVAGIRKFFNDKRFMEVETPMMQVIPGGASARPFVTHHNALDIDMYLRIA
PELYLKRLVVGGFERVYEINRNFRNEGISVRHNPEFTMIEFYMAYADYIDLMDLTEELLRTLAQDILGDTKIRYAKEGEE
GLTIDFGQPFQRLTMVDSILKFNPDVTPADLATLESAKAVAKHLHIELMKGWELGHVITAIFEETVEHMLLQPTFITEYP
AAVSPLARRNDVNPDVTDRFEFFIGGRELANGFSELNDAEDQAKRFQDQVNQKEAGDDEAMFYDADFVTALEHGLPPTAG
QGIGIDRLVMLFTNSHTIRDVILFPALRPQQK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 3e-146 60

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001140820.1 lysyl-tRNA synthetase VFG1668 Protein 2e-146 60