Gene Information

Name : entE (ASA_1839)
Accession : YP_001141663.1
Strain : Aeromonas salmonicida A449
Genome accession: NC_009348
Putative virulence/resistance : Virulence
Product : enterobactin synthase subunit E
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 1929314 - 1930984 bp
Length : 1671 bp
Strand : +
Note : bifunctional 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; activates the carboxylate group of 2,3-dihydroxy-benzoate forming (2,3-dihydroxybenzoyl)adenylate then catalyzes the acylation of holo-entB with 2,3-dihydroxy-benzoate adenylate

DNA sequence :
ATGACGCAGTCCAACCGCTTCCTGCCCTATACCCGCTGGCCCGAAGCCTTGGCGGAGCAGTATCGTGCCAAGGGCTATTG
GCGCGGCGAGCCCCTGACCGCCATGCTGGATCGCCAGTGCGAAGTGGCGCCGGATGCCACCGCCATCCTCTGCGGCGAAC
GCAGTTTCAGTTATCGCGAGCTGGACGCTGCCTCGAGCCGACTGGCCGGGCGATTGGCCCGCCATGGTCTGGGGGTCGGG
GACACAGCCCTGGTGCAGCTGCCCAATGTGGCCGAATTCTATCTGGTCTTCTTCGCCCTGCTCAAAGCAGGCATAGCGCC
GGTCAACGCGCTCTTTAGCCACAACCGGCTGGAGCTCATCTCTTACGCCGAGCAGATCACCCCCCGCCTCTTTATCGGCT
CGCTCGCCCATGCGCTGTTTGCCAATGGGGCGCGCCACAGTGACCTGCTGCGAGGCATAGGCGCCGAGCTGGTGCTGCTC
GATGGTGAGAGCGGGGAGCTGGGGCTGACTCATTGGCTGAGTGATCTGGCCAAGACCGAAGGATTGGCTGTCCCCGCCTA
TGGCCCGAGTCCTGCGGACGAGGTGGCCTTCTTCCAGCTCTCCGGCGGCAGCACCGGCACCCCCAAGCTGATCCCGCGCA
CCCATGACGACTACTACTACAGCGTGCGCCGCAGCAATGAAATCTGTGGACTCGGCCCCCATACCCGTTATCTCTGTGCC
CTGCCGGCTCCCCACAACTTCCCCTTGAGCTCCCCAGGTGCCCTCGGTGTGTTCGAGGCGGGCGGCGCCGTGGTGCTGGC
GCCGGATCCCGGCCCCATGAGCTGTTTTCCGCTGGTGGCCCGCCACAAGGTCAACCTCACTTCCCTGGTGCCACCGGCCG
TCTCTCTCTGGTTGCAGGCCACCGAATCTGACCCGTCCGTCCGCAGTCAGCTCGCGAGCCTCGACTTGCTGCAAGTAGGC
GGCGCCAAGCTGGCCGAAGCGGTGGCCAGAAAGATCACCCCGCTGCTCGGCTGCCAGCTGCAACAGGTGTTCGGCATGGC
GGAGGGGCTGGTCAACTACACCCGGCTGGACGATAGCGATGACAAAATAATCCACACCCAGGGCCGCCCCATGAGCCTGG
ATGACGAGGTGGGCATTCTGGACGAGGAGGGCCAGCCGGTCGCCCCGGGCCAGCCTGGCGCCCTGCATACCCGTGGCCCC
TACACCTTCCGCGGTTACTACCAGAGCCAGGCCCACAACGCCCGTGTGTTCGATGCAGATGGCTTCTACTGCTCCGGCGA
TCTGGTGGTGCAGGATGCCGATGGTTACCTCACTGTTGTGGGGCGGCAGAAGGATCAGATCAACCGGGGCGGCGAGAAAA
TCGCGGCGGAAGAGGTGGAGAACCAGCTGCTGCACCACCCGGCCATCACCCAGGCGGCCCTGGTCTCCATGCCCGACAGC
GCCATGGGGGAGAAGAGCTGCGCCTTCATCGTCAGCACGGATCCCGGGTTGAAGCCGCTGGCCTTGCGCAAGTTTCTGCG
TGAGCGCGGCGTGGCGGATTTCAAGCTGCCCGACCGTTTCGAAACTCTGGATGCCCTGCCCATGACGGCGGTGGGCAAGA
TTGACAAGCAGGGGCTGCGCGCGCAAATCGCCGAGTTAATCGCCGCCGGCCAGCCCCTGACCACAGCTTAA

Protein sequence :
MTQSNRFLPYTRWPEALAEQYRAKGYWRGEPLTAMLDRQCEVAPDATAILCGERSFSYRELDAASSRLAGRLARHGLGVG
DTALVQLPNVAEFYLVFFALLKAGIAPVNALFSHNRLELISYAEQITPRLFIGSLAHALFANGARHSDLLRGIGAELVLL
DGESGELGLTHWLSDLAKTEGLAVPAYGPSPADEVAFFQLSGGSTGTPKLIPRTHDDYYYSVRRSNEICGLGPHTRYLCA
LPAPHNFPLSSPGALGVFEAGGAVVLAPDPGPMSCFPLVARHKVNLTSLVPPAVSLWLQATESDPSVRSQLASLDLLQVG
GAKLAEAVARKITPLLGCQLQQVFGMAEGLVNYTRLDDSDDKIIHTQGRPMSLDDEVGILDEEGQPVAPGQPGALHTRGP
YTFRGYYQSQAHNARVFDADGFYCSGDLVVQDADGYLTVVGRQKDQINRGGEKIAAEEVENQLLHHPAITQAALVSMPDS
AMGEKSCAFIVSTDPGLKPLALRKFLRERGVADFKLPDRFETLDALPMTAVGKIDKQGLRAQIAELIAAGQPLTTA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 8e-84 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-84 43
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-84 43
ybtE CAA21394.1 - Virulence HPI Protein 8e-84 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-82 43
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 6e-84 43
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-82 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 9e-84 43
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-84 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
entE YP_001141663.1 enterobactin synthase subunit E VFG0932 Protein 4e-152 62
entE YP_001141663.1 enterobactin synthase subunit E VFG0168 Protein 7e-111 47
entE YP_001141663.1 enterobactin synthase subunit E VFG1817 Protein 1e-89 43