Gene Information

Name : lysS (Mflv_3544)
Accession : YP_001134806.1
Strain : Mycobacterium gilvum PYR-GCK
Genome accession: NC_009338
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3776598 - 3779903 bp
Length : 3306 bp
Strand : +
Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA

DNA sequence :
ATGACCGCAACCCGCCTCGTCAGGGCGCATCCCGAGTCCAGTCACCGGTGGGTGCCCGCGGCGGCCGGCTGGATCGTGGG
TGTGATCGCCACGTTGTCCCTGCTGGCGAGCGTGTCACCGCTGGTCCGCTCGATCATCCGCGTTCCCCGCGAGTTCGTCG
ACGACTACATCTTCAACTTCCCGGACACGAGCTTCGCGTGGGCGTTCGTGCTGGCGCTGCTCGCGGCCGCGCTCGCTGCC
CGCAAACGCATCGCCTGGTGGATCCTGACCGGCTACATGGTGGCCGCGGCCGTCTGGAACGTCACCGGACTGCTCGACGG
TGACCGGTGGTTCGAGGACGTCGGCGAGATCATCGGCCTGGGCTTCCACCTTGCCGCCATCGCGTCGCTGCTGTTGGCCC
GCAGGGAATTCTGGGCCCGGGTGCGCCCGGGCGCGCTCGTCAAGGCGGCAGTCACCCTGGTCGCGGGCCTGGGCGTCGGC
ACGCTGATCGGCTGGGGTCTGCTCGAACTGTTCCCCGGCACCCTGGCCCGGCCTGATCGCTTCTGGTACGCGCTCAACCG
GGTCGGCGCCTTCGCGGGGGCGGATGCCGGCGCGTTCTCTGGCCACCCGCACGTGCTGGTCAACGCCCTCCTCGGCCTGT
TCGGCGCGATGGCCCTGATGATCGCGGCGGTCGTGCTGTTCCAGTCACAGCGTGCGGACAACGCGCTGACCGGTGAGGAC
GAATCCGCCATCCGGGGTCTGCTGGAGCTGTACGGGAAGAACGATTCGCTGGGCTATTTCGCGACGCGTCGCGACAAGGC
GGTGGTCTTCGCTCCGAACGGACGGGCCGCGATCACCTACCGGGTCGAGGTCGGCGTGTGCCTGGCCAGCGGCGACCCGG
TCGGCGACCCGAAGGCGTGGCCGCAGGCCATCGAGGCGTGGCTGACGTTGTGTGAGACCTACGGGTGGGCGCCGGGTGTC
ATGGGCGCCAGCGCGACCGGCGCACAGGCGTTCCGCCAGGCGGGCCTGCACGCGCTGCAACTCGGCGACGAAGCGATCCT
GCATCCGGACGATTTCCGTCTCTCCGGACCCGAGATGCGCGCGGTGCGCCAAGCCGTCACCCGCGCTCGACGCGCCGGCG
TCGCCGTGCGCTTCCGCAGACACCGCGAGTTGTCACCCGACGAGATGGCCGAGGTGATCGCGCACGCCGACGCCTGGCGT
GACACCGAGGACGAGCGCGGATTCTCGATGGCGCTGGGCCGGCTCGGTGACCCCGCCGACGCCGACTGCCTCCTGGTGGA
GGCCGTACAGAACGGGACCCAGGTCGTCGCGATGCTGTCGCTGGTGCCGTGGGGCAGCAACGGTGTGTCACTGGACCTGA
TGCGGCGTGCCCCCCAATCCCCCAACGGCACCATCGAATTGATGGTCAGCGAGCTGTGCCTGCAGTCCGAGAGCATCGGC
ATCAGCCGGATCTCGCTGAACTTCGCGATGTTCCGCTCGGCGTTCGAGCAGGGCGCACAGCTCGGGGCCGGCCCGGTGGC
CCGGCTGTGGCGGTGGCTGCTCGTCTTCTTCTCCCGCTGGTGGCAGCTGGAGACGCTGTACCGCTCCAACATGAAGTACC
AGCCCGAGTGGGTGCCCCGCTATGCGTGTTACGAGGACGCCCGGCTGGTGCCCAGGGTCGGGGTCGCCTCGGTCATCGCC
GAAGGCTTTCTGGTGCTTCCCTTCTCGCGGCGCCACGATCAACCCCACACGGGGCACCACATCGCCGCACCGGGCGACCT
GATCGCCACCGGACGCCTGCACAGCGACGGAACGGCACCCGACCGCATCGGGCCCGTGGGCGACGGGGCCGACGACGACG
CCGCCCCGCGCCTGCCCGAGCAGGTCCGGGTGCGGATGGCGAAGCTCAGCGCCCTGCAGGACCGGGGCGTCGACGCCTAC
CCCGTGGGCAGCCCGCCCAGCCACACCATCGCCGAGGCGATCGCCGCCGAGGAAGGCACCGAGGTCACGGTGTCCGGCCG
GGTTCTGCGGGCCCGCGACTACGGTGGTGTGCTCTTCGGTCAGCTACGCGACTGGTCCGCCGAAACCCAGGTGGCGCTGG
ACAATTCGGCGCTGCTCGACGGAACCACCACCGACTTCACCCGCACCGTCGACCTCGGCGACCTGATCGAGGTGACCGGC
ACGATGGGCCACACCCGGTCGGGTTCGTGGTCGGTGCTGGTGACCCGGTGGCGGTTGATCGGCAAGTGCCTGCGGCCGCT
GCCCGACAAGTGGAAGGGGTTGACCGACCAGGAGGCGCGGGTGCGCGCCCGCTACGTCGACCTCGCGGTCAACACCGACG
CCCGCGAACTCATCAAGGCCCGCAGCGCCATTCTGCACGCCATCCGCGAAACCCTGGTCGGCAAAGGCTTTCTCGAGGTC
GAGACGCCGATCCTGCAGCAGATCCACGGCGGCGCCAACGCGCGTCCGTTCCTGACCCACATCAACGCCTACGACCTGGA
CCTGTATCTGCGGATCGCACCCGAGTTGTATCTGAAGAGGCTCTGTGTCGGGGGCGTCGAGCGGGTCTTCGAGCTGGGCC
GGGCGTTCCGGAACGAGGGGGTCGACTTCAGCCACAATCCCGAATTCACCCTGCTGGAGGCCTACCAGGCGCACGCGGAC
TACACCGTGTGGATCGACGGGTGCCGCGAGCTGATCCAGAACGCGGCCATGGCCGCCAACGGCTCCCACGTGTTCTTCCG
TCCGCGCGAGGACGGGGTCCTCGAACCGGTCGACATCTCCGGCCGATGGGCGGTCAAGACGGTTCACGGCGCGGTGTCGG
AGGCACTCGGCGAGCACATCGACGTCGACACCGACCTGGCGACGCTGCGCCGCCTCTGCGACAAGGCCGGCATCCCCTAC
CTGACGCACTGGGACGCCGGCGCGGTGGTCCTCGAGATGTACGAGCACCTCGTCGAGGACCAGACCACCGAACCCACCTT
CTACAAGGACTTCCCGACGTCCGTGTCGCCGCTGACCCGGCCGCACCGCAGCATCCCGGGGGTCGCCGAACGCTGGGACC
TCGTCGCGTGGGGGGTCGAGCTCGGCACCGCCTACAGCGAACTGACCGACCCTGTGGAGCAGCGCCGCAGGTTGCAGGAG
CAGTCGCTGCTGGCCGCAGGCGGCGACCCGGAGGCGATGGAGCTCGACGAGGACTTCCTGCAGGCGATGGAGTACGCGAT
GCCTCCGACCGGAGGCCTCGGAATGGGTGTCGATCGGGTGGTCATGCTGATCACCGGGCGCAGCATCCGCGAGACGCTGC
CGTTCCCGCTCGCGAAACCGAGGTAG

Protein sequence :
MTATRLVRAHPESSHRWVPAAAGWIVGVIATLSLLASVSPLVRSIIRVPREFVDDYIFNFPDTSFAWAFVLALLAAALAA
RKRIAWWILTGYMVAAAVWNVTGLLDGDRWFEDVGEIIGLGFHLAAIASLLLARREFWARVRPGALVKAAVTLVAGLGVG
TLIGWGLLELFPGTLARPDRFWYALNRVGAFAGADAGAFSGHPHVLVNALLGLFGAMALMIAAVVLFQSQRADNALTGED
ESAIRGLLELYGKNDSLGYFATRRDKAVVFAPNGRAAITYRVEVGVCLASGDPVGDPKAWPQAIEAWLTLCETYGWAPGV
MGASATGAQAFRQAGLHALQLGDEAILHPDDFRLSGPEMRAVRQAVTRARRAGVAVRFRRHRELSPDEMAEVIAHADAWR
DTEDERGFSMALGRLGDPADADCLLVEAVQNGTQVVAMLSLVPWGSNGVSLDLMRRAPQSPNGTIELMVSELCLQSESIG
ISRISLNFAMFRSAFEQGAQLGAGPVARLWRWLLVFFSRWWQLETLYRSNMKYQPEWVPRYACYEDARLVPRVGVASVIA
EGFLVLPFSRRHDQPHTGHHIAAPGDLIATGRLHSDGTAPDRIGPVGDGADDDAAPRLPEQVRVRMAKLSALQDRGVDAY
PVGSPPSHTIAEAIAAEEGTEVTVSGRVLRARDYGGVLFGQLRDWSAETQVALDNSALLDGTTTDFTRTVDLGDLIEVTG
TMGHTRSGSWSVLVTRWRLIGKCLRPLPDKWKGLTDQEARVRARYVDLAVNTDARELIKARSAILHAIRETLVGKGFLEV
ETPILQQIHGGANARPFLTHINAYDLDLYLRIAPELYLKRLCVGGVERVFELGRAFRNEGVDFSHNPEFTLLEAYQAHAD
YTVWIDGCRELIQNAAMAANGSHVFFRPREDGVLEPVDISGRWAVKTVHGAVSEALGEHIDVDTDLATLRRLCDKAGIPY
LTHWDAGAVVLEMYEHLVEDQTTEPTFYKDFPTSVSPLTRPHRSIPGVAERWDLVAWGVELGTAYSELTDPVEQRRRLQE
QSLLAAGGDPEAMELDEDFLQAMEYAMPPTGGLGMGVDRVVMLITGRSIRETLPFPLAKPR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 9e-153 42
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-153 42
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-153 42
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 3e-153 42