Gene Information

Name : Mflv_3213 (Mflv_3213)
Accession : YP_001134478.1
Strain : Mycobacterium gilvum PYR-GCK
Genome accession: NC_009338
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 3410349 - 3411752 bp
Length : 1404 bp
Strand : +
Note : TIGRFAM: mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; pyridine nucleotide-disulphide oxidoreductase dimerisation region; FAD dependent oxidoreductase; KEGG: cjk:jk1442 putative mercuric reductase

DNA sequence : 
ATGAGTCAGGGATTGGACCTCGCGGTCATAGGTTCCGGCGGCGCGGCGATGGCCGCGGCGATCCGCGCTACCGCGCTCGG
CAAGTCCGTCGTCATGATCGAGCGCGGCATCTTCGGTGGCACGTGTGTCAACACCGGCTGCGTGCCGTCAAAGGCCCTGA
TCGCAGCGGCCGAGGCCCGGCATACCGCAGCCGACACTGCCCGGTTCCCGGGGATCGCCACCACGGCCGGCCCGGTGGAC
ATGGCGGCCCTGATCGCCGGCACGCACGATCTGGTGGAGTCGCTGCGCTCAGAGAAGTACCTCAACGTGGCCGAATCGTA
TGGCTGGCAGCGCATTCAGGGCCAAGCGCGGTTCGCCGGAACCCCGGACGCGCCCGTCATCGAGGTCGGCGATGCCACCA
TCGAGGCCGAGCACTACCTGATCGCCACCGGCGCGAACCCAGTCATCCCGCCCGCATTCGAGGGCGTCGCCTACCTGACC
TCGACCACCGCGATGGAAGTCACCGAGGTCCCGGAGTCGCTGCTGGTGATCGGCGGCGGCTACGTCGCGTTGGAGCAGGC
GCAACTGTTCGCCCGGCTCGGGTCAACGGTGACGGTGCTGGTCCGCTCCACGCTGGCATCGAAGGAAGAGCCGGAAGTCG
GCATGGCGCTGCTGGAGGTGTTCGCCGACGACGGCATCCGGGTGGTGCGCCGCGCCACGGTGAGCGAGGTCGAGCAGGCC
GACGATCAGGTCACGGTCACCGCGACCATCACCGGCGGAACGCAGCAGTTCCGTGCCGCGAAAGTCCTCGTCGCCACCGG
CCGCCGTCCGAACACCGACGGCCTGAATCTCGAAGCGGTGCAGGTCAAAACCGGCGAGAACAACGAGGTCGTGGTGAGCG
ACGGGCTGCAGTCGTCGAACCCACGGATCTGGGCGGCCGGCGACGTGACCGGGCACCGCGAGTTCGTCTACGTCGCCGCC
CATCACGGCGCGATGGTCGCCGACAACATCTTCACCGACGCCGGCCGCAGGGTCGACTACCGCCATCTGCCCCGCGTGAC
GTTCACCAGCCCCGCGGTCGGTGCGGCCGGGACGACCGAGGCCGAGCTCCTCGCCGCCGGGACACGGTGCGACTGCCGGG
TGCTGCCGCTAAAACATGTGCCGCGTGCGGTGGTCAACCGAGACACCCGCGGTTTCATCAAACTCGTCGCCGACGCCGGC
ACCGGCCGCATCCACGGCATCACCGCCGTCGCCAAGGATGCTGGCGAGATCGCCGCCGCCGCGGTCTACATCCTCGACGC
CGCGATGACCGTCGACCAGGTCGCCGGGTCCTGGGCCCCGTATCTGACCATGGCCGAAGGCATCAAAATCGCCGCCCAGT
CCTTCAGCGCCGATATGTCCCGACTGTCCTGCTGCGCATCCTGA

Protein sequence : 
MSQGLDLAVIGSGGAAMAAAIRATALGKSVVMIERGIFGGTCVNTGCVPSKALIAAAEARHTAADTARFPGIATTAGPVD
MAALIAGTHDLVESLRSEKYLNVAESYGWQRIQGQARFAGTPDAPVIEVGDATIEAEHYLIATGANPVIPPAFEGVAYLT
STTAMEVTEVPESLLVIGGGYVALEQAQLFARLGSTVTVLVRSTLASKEEPEVGMALLEVFADDGIRVVRRATVSEVEQA
DDQVTVTATITGGTQQFRAAKVLVATGRRPNTDGLNLEAVQVKTGENNEVVVSDGLQSSNPRIWAAGDVTGHREFVYVAA
HHGAMVADNIFTDAGRRVDYRHLPRVTFTSPAVGAAGTTEAELLAAGTRCDCRVLPLKHVPRAVVNRDTRGFIKLVADAG
TGRIHGITAVAKDAGEIAAAAVYILDAAMTVDQVAGSWAPYLTMAEGIKIAAQSFSADMSRLSCCAS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 1e-79 44
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 9e-80 44
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 2e-63 44
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 6e-64 43
merA ABQ57371.1 MerA Not tested SGI1 Protein 6e-66 43
merA AGK07021.1 MerA Not tested SGI1 Protein 6e-66 43
merA AGK07079.1 MerA Not tested SGI1 Protein 6e-66 43
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 1e-64 43
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 1e-64 43
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 2e-63 42
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 2e-63 42
merA AFG30120.1 MerA Not tested PAGI-2 Protein 2e-63 42

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Mflv_3213 YP_001134478.1 mercuric reductase BAC0651 Protein 6e-80 44
Mflv_3213 YP_001134478.1 mercuric reductase BAC0652 Protein 2e-66 44
Mflv_3213 YP_001134478.1 mercuric reductase BAC0653 Protein 3e-63 42
Mflv_3213 YP_001134478.1 mercuric reductase BAC0650 Protein 1e-61 42