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Name : lysS (GTNG_0074) Accession : YP_001124206.1 Strain : Geobacillus thermodenitrificans NG80-2 Genome accession: NC_009328 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : - Position : 84588 - 86072 bp Length : 1485 bp Strand : + Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGAGCCATGAAGAATTGAACGACCAATTGCGTGTCCGCCGGGAAAAGTTAAAAAAAATCGAAGAGCTAGGTGTCGACCC GTTTGGCAAACGGTTCGAGCGCACGCATAAAGCAGAAGAACTGTTTAAACTGTACGGCGATTTGTCCAAAGAAGAACTTG AAGAGCAGCAAATTGAAGTCGCTGTCGCCGGCCGCATTATGACGAAACGCGGTAAAGGAAAAGCAGGATTTGCTCACATT CAAGACGTCACAGGGCAAATTCAAATTTATGTCCGCCAAGACGATGTCGGCGAACAGCAATATGAGCTGTTTAAAATCTC TGACCTTGGTGATATCGTCGGTGTGCGCGGCACTATGTTCAAAACAAAAGTCGGCGAGCTTTCCATCAAAGTGTCATCAT ATGAATTTTTAACAAAAGCATTGCGTCCATTGCCGGAAAAATACCATGGTTTAAAGGACGTCGAACAACGTTACCGCCAA CGTTATCTCGACTTAACTATGAATCCGCAAAGTAAGCAGACGTTTATCACCCGTAGTCTCATTATTCAATCGATGCGGCG TTATCTCGACAGCCAAGGTTATTTGGAAGTCGAAACACCGATGATGCACGCCATAGCAGGTGGTGCGGCTGCACGTCCGT TTATTACGCACCATAATGCCCTTGATATGACACTTTATATGCGAATCGCCATCGAACTCCATTTAAAACGGCTCATCGTC GGCGGTTTGGAAAAAGTGTATGAAATCGGACGCGTCTTCCGGAATGAGGGGATTTCCACCCGTCACAATCCGGAGTTTAC GATGCTTGAACTGTACGAGGCATATGCCGACTTCCGTGACATCATGAAATTGACAGAAAACTTAATTGCTCACATTGCCA CGGAAGTGCTTGGCACGACGAAAATTCAATACGGCGAACATACCGTCGATTTAACGCCTGAATGGCGGCGACTTCATATG GTCGATGCGATTAAAGAATACGTCGGCGTTGATTTCTGGCGGCACATGGACGACGAGGAAGCGCGGGCGTTGGCGAAAGA ACATGGGGTCGAAATCGCCCCGCACATGACGTTTGGTCATATCGTCAATGAATTTTTTGAACAAAAAGTCGAGTCGCAAC TCATCCAACCGACGTTCATTTATGGCCACCCTGTCGAAATTTCGCCGTTAGCTAAGAAAAACCCGGACGATCCACGCTTT ACCGATCGATTTGAGCTATTTATCGTTGGACGTGAACATGCGAACGCGTTTACGGAACTAAACGATCCGATCGACCAGCG CCAACGTTTCGAAGCACAGTTGAAAGAACGTGAACAAGGGAACGATGAAGCGCACGAAATGGACGAAGATTTCCTCGAAG CGCTCGAGTACGGTATGCCTCCAACAGGCGGACTCGGCATCGGCGTTGACCGTCTAGTCATGCTCTTGACTAACTCTCCG TCCATTCGGGATGTGTTACTCTTCCCGCAAATGCGTCATAAATAA Protein sequence : MSHEELNDQLRVRREKLKKIEELGVDPFGKRFERTHKAEELFKLYGDLSKEELEEQQIEVAVAGRIMTKRGKGKAGFAHI QDVTGQIQIYVRQDDVGEQQYELFKISDLGDIVGVRGTMFKTKVGELSIKVSSYEFLTKALRPLPEKYHGLKDVEQRYRQ RYLDLTMNPQSKQTFITRSLIIQSMRRYLDSQGYLEVETPMMHAIAGGAAARPFITHHNALDMTLYMRIAIELHLKRLIV GGLEKVYEIGRVFRNEGISTRHNPEFTMLELYEAYADFRDIMKLTENLIAHIATEVLGTTKIQYGEHTVDLTPEWRRLHM VDAIKEYVGVDFWRHMDDEEARALAKEHGVEIAPHMTFGHIVNEFFEQKVESQLIQPTFIYGHPVEISPLAKKNPDDPRF TDRFELFIVGREHANAFTELNDPIDQRQRFEAQLKEREQGNDEAHEMDEDFLEALEYGMPPTGGLGIGVDRLVMLLTNSP SIRDVLLFPQMRHK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 1e-121 | 51 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 2e-92 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001124206.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 8e-122 | 51 |
