Gene Information

Name : Dred_1527 (Dred_1527)
Accession : YP_001112881.1
Strain : Desulfotomaculum reducens MI-1
Genome accession: NC_009253
Putative virulence/resistance : Resistance
Product : heavy metal translocating P-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 1657020 - 1659371 bp
Length : 2352 bp
Strand : +
Note : TIGRFAM: ATPase, P-type (transporting), HAD superfamily, subfamily IC; cadmium-translocating P-type ATPase; heavy metal translocating P-type ATPase; PFAM: Haloacid dehalogenase domain protein hydrolase; Heavy metal transport/detoxification protein; E1-E2

DNA sequence :
TTGGATAAGGGAGGTATTACTGTGCAAAAGACACATGCTGATAAACAAAAAGAGCAATGTGGCTGTGGCTGTGAAGGCAA
AAGAATCAACCCAACACGAGGTTTTTCGATGGGGAAAATCAACTACAAATCTGCACTGACGAAGGACTTTGCCTTACCTA
GTTCCCCATTTATTACTGAAGAAAAAGCAGACCAAAAGAAAACAGAACTCCGTTTAAGCGGTCTGGATTGTGCCGATTGT
GCTGCCAAACTGGAGAGGAAAATTCAAGCCCTGCCTGGTGTGGCGGAAGCAAAGATTAATTTTGGTGCAGGTAAGATCAC
CGTTTGCCATACGACCCCGGCAGAGACCATTTTGCAGGCAATTAAAACAGCTGGCTACCAAGGGCGTTTAGCTAACGAAG
CAAGGAAACCACCCAAGCAAAGCGTCTTTGAAGATACTAAGCTGGTTCTAACGGGTTTTTCAGGTCTTATGGTAGCAGTG
GGTTTTTTCCTCTCGTTTTCAAATATATCGGAAACTCTAGTAAATGGAGTCTATCTTGCCGCTATCTTAAGCGGTGGATT
TTATACCGCCCGAAGTGGTCTTACTTCTCTTAAGTCTGTGTCATTGGATATGAATTTCCTTATGACTGTGGCGGTGGTGG
GCGCTGTAGCCATTGGAGAATGGGCTGAAGGGGCAACGGTGGTATTCCTTTTTGCTCTGGGCAATACCCTGCAGGCCTTT
ACAATGGAAAAAACCCGTAACTCCATTCGGGCATTGATGGATCTCTCCCCTAAAGATGCTCTGGTACTTCGTAACGGCCA
GGAACTACGGTTGCCAGTTGAGGAGCTTAGAATTGATGACATCATAATTGTAAAACCCGGCGAATGTATTCCTATGGATG
GTGAAGTGGTGGCAGGGACTACTGATGTTAATCAGGCGCCCATCACTGGAGAATCCATGCCAATTGAGAAAACTGTTGGA
CATGAGGTTTACGCTGGTACGATGAACGGACATGGGGCCATTGAGGTTAAAGTTACCAAGTTGGTGGAAGATACCACATT
GGCGAAAATCATAAACCTTGTGGAAGAGGCCCAGGCTCAAAAAGCACCCTCCCAACAGCTTGTTGATGTATTCGCTAAGT
ACTATACCCCTGCAGTGGTCATAGGCGCTGTACTAATAGCTTTGGTTCCTTGGTTATTCTTCGCACAGCCCTTTCAACTC
TGGATTGAACGGGCGCTGATACTGTTGGTAATTTCTTGTCCCTGCGCCCTTGTTATATCCACTCCTGTATCCATTGTGGC
TGCCATCGGCAGTGCTGCCCGAAAAGGGGTATTAATTAAAGGGGGAGCTTATCTGGAGGAAGCGGGGTCTTTAAAGGTAA
TTGCCTTTGATAAAACCGGCACCCTCACCCGGGGCAGGCCGGAAGTGGCTGCAGTAATACCCGTGGATGGAGTAAGCTCC
CAAAGGGTGATTGAACTTGCTGCTGCCATCGAAAAACGTTCCCAGCATCCATTGGCCGAGGCCATTTTGAGGTATGCTAA
GGAGAAGGAGATTAAAGTACCCATAGGAACAGAGTTTCAATCCTTTACAGGTAAAGGTGCTGCGGCAATTGTGGATGGTA
TGGTCTGTTACATTGGTAACAGTCGTTTGTTTGAAGAGTTAAATCTTTTCCCAGCCCCCCTGCAGGAACAGATTACTGTG
CTGCAGAATCAAGGAAAAACAGTGATGATTTTGGGCAGTGGACATGAAGTCCTGGGGCTTATTGCGGTGGCTGACCAAAT
TCGCGAAAGCAGCCGGGCAGCCGTGGCAGGGCTGCGTCAGGCTGGTATTAGTCAACTGGTAATGCTAACTGGTGATAACA
CCGGAACCGCCAGGATAATTGCCCAGGAATTAGGGATTGATGATTACCGGGCAGAACTTTTACCTGAAAATAAATTAAAG
GCCATTCAACAATTACAGCAGGAGTATGGTAAGGTAGGGATGGTGGGTGATGGCATCAACGATGCACCAGCCCTGGCCAC
TGCCGCTGTAGGCATTGCTATGGGGGGAGCCGGCACCGATACTGCTTTGGAAACTGCGGACATTGCATTGATGGCCGACG
ATTTAAGTAAACTACCCTATGCCATGCACCTGAGTAAACAGACCCTGCGGATTATTAAACAGAATATCTGGTTTTCCCTG
GCTATAAAAGCTGTGTTTATTGCTGCTACCTTCTTGGGTTTTGCCAACCTGTGGATGGCTGTATTTGCCGATACCGGAGC
GGCCCTTTTGGTGATTGCTAATGGGATGAGATTAATGAAGGTGGAGGACCCCTATACAAGGGATAGCTTGGATTCCCGTA
AATTATCATCCCAAACCTGTACCAGTGTGTAA

Protein sequence :
MDKGGITVQKTHADKQKEQCGCGCEGKRINPTRGFSMGKINYKSALTKDFALPSSPFITEEKADQKKTELRLSGLDCADC
AAKLERKIQALPGVAEAKINFGAGKITVCHTTPAETILQAIKTAGYQGRLANEARKPPKQSVFEDTKLVLTGFSGLMVAV
GFFLSFSNISETLVNGVYLAAILSGGFYTARSGLTSLKSVSLDMNFLMTVAVVGAVAIGEWAEGATVVFLFALGNTLQAF
TMEKTRNSIRALMDLSPKDALVLRNGQELRLPVEELRIDDIIIVKPGECIPMDGEVVAGTTDVNQAPITGESMPIEKTVG
HEVYAGTMNGHGAIEVKVTKLVEDTTLAKIINLVEEAQAQKAPSQQLVDVFAKYYTPAVVIGAVLIALVPWLFFAQPFQL
WIERALILLVISCPCALVISTPVSIVAAIGSAARKGVLIKGGAYLEEAGSLKVIAFDKTGTLTRGRPEVAAVIPVDGVSS
QRVIELAAAIEKRSQHPLAEAILRYAKEKEIKVPIGTEFQSFTGKGAAAIVDGMVCYIGNSRLFEELNLFPAPLQEQITV
LQNQGKTVMILGSGHEVLGLIAVADQIRESSRAAVAGLRQAGISQLVMLTGDNTGTARIIAQELGIDDYRAELLPENKLK
AIQQLQQEYGKVGMVGDGINDAPALATAAVGIAMGGAGTDTALETADIALMADDLSKLPYAMHLSKQTLRIIKQNIWFSL
AIKAVFIAATFLGFANLWMAVFADTGAALLVIANGMRLMKVEDPYTRDSLDSRKLSSQTCTSV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 5e-166 50
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 4e-166 50
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 3e-164 49
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 1e-129 46

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Dred_1527 YP_001112881.1 heavy metal translocating P-type ATPase BAC0298 Protein 1e-125 44
Dred_1527 YP_001112881.1 heavy metal translocating P-type ATPase BAC0092 Protein 5e-102 43
Dred_1527 YP_001112881.1 heavy metal translocating P-type ATPase BAC0124 Protein 1e-102 43