Gene Information

Name : lysS (SACE_4738)
Accession : YP_001106931.1
Strain : Saccharopolyspora erythraea NRRL 2338
Genome accession: NC_009142
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 5296227 - 5299523 bp
Length : 3297 bp
Strand : +
Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA

DNA sequence :
GTGGAGCATGCGCGGCGGTTCCGGTGGCGCGACCGCGTCCCGGGCGCGGCCGCGGGGCTCGTGTTCGCGATCGCCCTGCT
GTGCTCGTTCGCGGCCGTCGGCCTGGTGCTGCACGGCCACGTCCAGCCGGTGCGCCGGTTCGTCGACGACGTCGTCTTCC
CCGCCCCGCCCAACCTCGCCTACGGGGCCTTCCTGGTGGTGCTCGCGGCGGCGCTGAAGCGGCGCAAGTGGGTCGCGCAC
CGGATTCTCGCGGCGATCGTGCTGCTCCAGCTGCTCGTGGACCTGGCGGTGCTGTTGGCCGCGGGCGGCGAGGGTTCGCT
GTGGATCGACGAGATGGGCACGTTGCGGCAGGTGCCGGTCAGCCCGTGGCCGTTCGGGGTGAACGTCGCGGTGAGCCTCG
CCGCGCTGACGCTGCTGGTGTGGGCGCGGGGCGAGTTCTACGGGCGCACCGGCACCGGCAGCGTCCGCCGCGCGGCGGCC
ACCTTCTGCGGACTCGTCGCGGCGTCCGTGGCGCTGGGCTTCGGCCTGGTGACGGTGTTCCCCGGTTCGCTGGACGACCC
GCGGGAACGCCTGACCTGGACGGTGGAGCGGGTGCTGGGCGGCGCGGTGGTCTTCGACGTCACCCGCGTCGGCCACGCCC
CGGGCTGGGTCAACCTGCTGCTCGGGCTGTTCGGCAGCGCCGCGCTGTTCGCGACGCTGTTCGTCCTCTTCGCCGCCCAG
CGGGTGGCCGCGGCGCTGACGCCCGAGCAGGAGCAGCGGATCCGGCAGCTGCTGGACGACCGCGACGGCGACTCGCTGGG
CTACTTCGCCACCAGGCGCGACCGGTCGGCGCTGTTCTCGGCCAGCGGCAAGGCGGCGATCAGCTACCGTCCGGTCGCCG
GGGTGTGCCTGGCGGGCGGCGACCCGGTCGGCGACCGCGAGGCGTGGGCCCCGGCGATCCAGGACTGGCTGGCGATGTGC
CGGGAGTACACCTGGATGCCCGCGGCGATGGGGCTCAGCGAGCCCGCTGCCGCCGCGTTCGCCCGGGCCGGGCTGAAGGT
GCTGCAACTTGGCGACGAGGCGGTGCTGCACACCGCCGAGTTCGGCCTGGCGGGCCGGGACATGCGACCGGTGCGGCAGG
CGGTCAACCGGGTCCACCGCGCCGGGTACCGCACGCGCGTCAGGCGGCATCGGGACGTGCCTGGAGCCGAGATGCGCGAG
ATCGTCGAACTGGTCGACCGGTGGCGCACCAGCGGCACCGAGCGCGGCTTCTCGATGGCGCTGGGCAGGCTCGGCGACCC
GCAGGACGGCGACTGCGTGCTCGTGGAGGCCGTCGGACCGGACGGACGGCGGCACGCCCTGCTGTCGCTGGTCCCGTGGG
GCCGCGACGGTCTGTCGCTGGACCTGATGCGCCGCGATCCGGACGCCGACAACGGGCTGGTCGAGCTGATGGTCTCGGGC
CTGATGCGCACGGCGCCGGTACTCGGTGTGACGCGGGTGTCGCTGAACTTCGCGGTGTTCCGCTCGGCGTTCGAGGAAGG
CGCGCGGCTGGGCGCGGGCCCGGTGCTGCGGGCTTGGCGGGGACTGCTGCTGTTGCTGTCGCGCTGGTGGCAGCTGGAGT
CGCTGTACCGGTCGAACGTGAAGTACCAGCCGCACTGGTATCCCCGCTACATCGCCTTCGCCGAGCACCGCGAGCTCGCC
CGCGTGGGCCTGGCCTCGGCCATCGCCGAGGGCTTCGTACCCGCCTTCGGCCACCGCCGGGCGATGACGGTGTCCGGCGC
GCGGCCCGAGGCCTCGGCGACGGCGCCGACCGCACCGCAGCCGACCGCCGAACCGGAGCAGCAGCAGGTGCGGCGGCGCA
AGCTCGCCGAGCTCCGCGAGGCAGGCGTCGACCCGTACCCGCCCGAGGTGCCCCGCACGCAACCGTGCCGCGCGGTGGCC
GAGCACCACGGTGGGCTGGAGCCCGGAACCCGCACCGGCGAGACCACGTCGGTGGCCGGCCGGGTGATGCGCCTGCGAGA
CCACGGCGGCCTGTGCTTCGCGACCGTGCGCGACTGGTCCGGCGACCTCCAGGTGCTGCTCACCGCCGACGACAGCGGCC
ACGACCTGCTGGTGGCCTGGCGCGGCGAGGTCGACCTCGGCGACCAGGTGTCGGTGACCGGAGAGGTGGTGACCTCCGAC
CGCGGCGAGCTGACGGTGCTGGCCAAGAGCTGGACCCTGGCGGGCAAGTGCCTGCACCCGCTGCCCGACAAGCACCGCGG
TCTGTCCGATCCGCACTCGCGCGTCCGCGCCCGCCACCTCGACCTGATCACGCGGCCGGAGGCGCGCTCCGTGCTGCGGG
CGCGCAGCGCGGCCACCCACGCGCTGCGGGACGTGCTCGTGCGGTCCGACTTCGTCGAGGTCGAGACGCCGGTGCTGCAA
CCGGTCCGCGGCGGGGCGAACGCGCGGCCGTTCAGCACCCACATCAACGCCTACGACCTGCGCCTGTACCTGCGGATCGC
CCCGGAGCTCTACCTCAAACGGCTCTGCGTCGGCGGCGTCGAGCGGCTGTTCGAGATCGGCCGGGCGTTCCGCAACGAAG
GCGTGTCGCACAAGCACAACCCGGAGTTCACGATGCTGGAGGCCTACCAGGCGTTCGGCGACTACACCACGATGCGCGAG
CTGTGCCGGGAGCTGGTGGTGGCCGCCGCGGTCGCCGCGACCGGTTCGACCGTCGTGACCGGCCGCGACCGGCTCGGCCG
CGATGTCGCGACCGACCTCGCGGACGACTGGCCGGTGATCGGCGTGCACGAGGCGGTGTCGGCCGCGACCGGCGAGGAGG
TCACCGCGGACACACCCGTGCGACGGCTCCGGCGCCTCTGCGACGACAACGGCATCGCGCACCTGCGGGACTGGGGACGC
GGGGCGGTGCTGCTGGAGCTCTACGAGCGGTTGGTGGAGGCCGAGACCACCGGGCCGACCTTCTACCGCGACTTCCCGGC
CGAGGTCTCGCCGCTGGCCCGCCCGCACCGCCACGACGCCAGGCTTGCCGAGCGCTGGGACCTGGTCGCCTTCGGTACCG
AGCTCGGCACCGGCTACTCGGAGCTGACAGACCCGGTGGAGCAGCGGCGGCGGCTGGCCGACCAGGCCCGGCTCGCCGCC
GAGGGCGACGCCGAGGCCATGGACGTCGACGAGGACTTCCTCACCGCGCTGGAGTACGCCATGCCGCCCACCGGCGGCCT
CGGGCTCGGCGTCGACCGCCTGGTCATGCTGCTCACCGGCCGCTCCATCCGCGACACGCTCGCCTTCCCGCTGGTCAAGC
CGCAACGCGCGACCTGA

Protein sequence :
MEHARRFRWRDRVPGAAAGLVFAIALLCSFAAVGLVLHGHVQPVRRFVDDVVFPAPPNLAYGAFLVVLAAALKRRKWVAH
RILAAIVLLQLLVDLAVLLAAGGEGSLWIDEMGTLRQVPVSPWPFGVNVAVSLAALTLLVWARGEFYGRTGTGSVRRAAA
TFCGLVAASVALGFGLVTVFPGSLDDPRERLTWTVERVLGGAVVFDVTRVGHAPGWVNLLLGLFGSAALFATLFVLFAAQ
RVAAALTPEQEQRIRQLLDDRDGDSLGYFATRRDRSALFSASGKAAISYRPVAGVCLAGGDPVGDREAWAPAIQDWLAMC
REYTWMPAAMGLSEPAAAAFARAGLKVLQLGDEAVLHTAEFGLAGRDMRPVRQAVNRVHRAGYRTRVRRHRDVPGAEMRE
IVELVDRWRTSGTERGFSMALGRLGDPQDGDCVLVEAVGPDGRRHALLSLVPWGRDGLSLDLMRRDPDADNGLVELMVSG
LMRTAPVLGVTRVSLNFAVFRSAFEEGARLGAGPVLRAWRGLLLLLSRWWQLESLYRSNVKYQPHWYPRYIAFAEHRELA
RVGLASAIAEGFVPAFGHRRAMTVSGARPEASATAPTAPQPTAEPEQQQVRRRKLAELREAGVDPYPPEVPRTQPCRAVA
EHHGGLEPGTRTGETTSVAGRVMRLRDHGGLCFATVRDWSGDLQVLLTADDSGHDLLVAWRGEVDLGDQVSVTGEVVTSD
RGELTVLAKSWTLAGKCLHPLPDKHRGLSDPHSRVRARHLDLITRPEARSVLRARSAATHALRDVLVRSDFVEVETPVLQ
PVRGGANARPFSTHINAYDLRLYLRIAPELYLKRLCVGGVERLFEIGRAFRNEGVSHKHNPEFTMLEAYQAFGDYTTMRE
LCRELVVAAAVAATGSTVVTGRDRLGRDVATDLADDWPVIGVHEAVSAATGEEVTADTPVRRLRRLCDDNGIAHLRDWGR
GAVLLELYERLVEAETTGPTFYRDFPAEVSPLARPHRHDARLAERWDLVAFGTELGTGYSELTDPVEQRRRLADQARLAA
EGDAEAMDVDEDFLTALEYAMPPTGGLGLGVDRLVMLLTGRSIRDTLAFPLVKPQRAT

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 5e-152 46
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 2e-151 46
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 5e-152 46
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 5e-152 46