Gene Information

Name : lysS (Shew_0812)
Accession : YP_001092943.1
Strain : Shewanella loihica PV-4
Genome accession: NC_009092
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 933950 - 935452 bp
Length : 1503 bp
Strand : +
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACTGAACAAACTCAAGACGAAAACAAGTTAATTGCAGAGCGCCGTGCCAAGTTAGATCACGTTCGCGCTTCTTGCCC
TGCTAACGGTCACCCGAACAACTTCGATCGAAAACATAAGGCTGCCGACATCCAGGCCGAATATGGTCAATACAGCAAGG
AAGAGCTGGAAGAGATGAATGTCCAGCGCTCTATCGCCGGTCGTATCATGGCTAAGCGTGGCCCCTTCTTGGTTATCCAG
GACGTGAGCGGTCGCATCCAGGCCTATGCCGGTAAAGACGTTCAGAAAGATCTTAAGGCCAAGTATCAAGGTCTGGATAT
CGGTGACATTATCGGTGTGACCGGCCAGCTGCACCTGTCTGGCAAGGGCGACCTCTATGTCAACATGGAAGAGTACCAGC
TGCTGACCAAGGCGCTGCGTCCGCTGCCTGAGAAGTTCCACGGCCTGACTGACCAGGAGACCCGCTACCGTCAGCGTTAT
GTTGACCTCATCGTGAACGAAGAGTCACGTGCGGCCTTCATTATGCGCTCTAAAGTGGTTACCGCTATCCGTAACTTCAT
GGTAAGCAAAGAGTTTATGGAAGTGGAAACCCCTATGATGCACAGCATCCCGGGTGGTGCCTCTGCGCGTCCTTTCATTA
CTCACCACAATGCGCTGGATATCGAGATGTACCTGCGTATCGCGCCTGAGCTATACCTCAAGCGTCTGGTGGTTGGTGGT
TTCGAGCGCGTGTTCGAGATCAACCGTAACTTCCGTAACGAAGGCCTGTCGCCACGCCATAACCCAGAATTCACCATGAT
GGAATTCTATATGGCCTATGCCGACTACAAAGATCTGATGGACCTGACCGAAGAGATGCTCTCTTCGATCGCCAAAGATC
TGCTGGGTGATACCAAGCTGCCATACGGCGAGCACACTATCGACTTCGGCGGTCCATACGAGCGTCTGAGCATGCTGGAT
GCGATCAAGAAGTACAACCCAGACAACGAGACGATTCAGTCTATGACCTATGAAGAGGTTAAAGACGTCGAGTTTATGCG
TAACCTGGCCAAGAGCCTGGGCATGACTATCGAGAAGTTCTGGACCTGTGGTCAGCTGCTGGAAGAGATCTTCGGTGAAA
CCGCCGAGCCTAAGCTGATGCAGCCTACCTTCATCACTGGCTACCCGGCAGATATCTCACCGCTGGCCCGCCGTAACGAT
GAAAACCACTTCATCACCGACCGTTTCGAGTTCTTCATCGGCGGTCGCGAAGTGGCCAACGGCTTCAGCGAGCTGAACGA
TGCGGAAGATCAAGACAAGCGCTTCAAGGCGCAGGTCGATGCTAAAGATGCGGGCGACGATGAAGCCATGTTCTATGACG
CCGACTACATCACGGCGCTGGAGCACGGTCTGCCACCGACTGCCGGTCAGGGTATCGGTATCGACCGTCTGGTGATGTTG
TTTACCAACACCCACACCATTCGTGACGTGATCCTCTTCCCGGCGATGCGTCCTCAGGGATAA

Protein sequence :
MTEQTQDENKLIAERRAKLDHVRASCPANGHPNNFDRKHKAADIQAEYGQYSKEELEEMNVQRSIAGRIMAKRGPFLVIQ
DVSGRIQAYAGKDVQKDLKAKYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEESRAAFIMRSKVVTAIRNFMVSKEFMEVETPMMHSIPGGASARPFITHHNALDIEMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEEMLSSIAKDLLGDTKLPYGEHTIDFGGPYERLSMLD
AIKKYNPDNETIQSMTYEEVKDVEFMRNLAKSLGMTIEKFWTCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRND
ENHFITDRFEFFIGGREVANGFSELNDAEDQDKRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 9e-140 57

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001092943.1 lysyl-tRNA synthetase VFG1668 Protein 5e-140 57