Gene Information

Name : fliI (Shew_1369)
Accession : YP_001093497.1
Strain : Shewanella loihica PV-4
Genome accession: NC_009092
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 1578763 - 1580103 bp
Length : 1341 bp
Strand : +
Note : involved in type III protein export during flagellum assembly

DNA sequence :
ATGAAGACACGTCAAAACCGGCTTCTTGATAAGTTAAGGCAGCATACCCAGGCGGTCGCCCCTTTTATGCCGGAGGCCTC
GGGCCAGCTGGTGCGCGTGGTAGGCCTAACGCTGGAGGCCACCGGCTGCAAGGCGCCCGTGGGCAGTCTGTGTAGCATAG
CCACCATGGCAGGAGATCTGATCGCCGAGGTGATAGGCTTCGATGACAAGCTACTCTATCTGATGCCCATCGAGGAGCTC
AGCGGCGTGATGCCTGGTGCCAAGGTGACGCCGCTCGGCGAGCAGACTGGGCTGAATGTCGGCATGGGCCTGCTTGGCCG
CGTGCTCGATGGCAATGGTGCGCCGATAGACGGCTTGGGGCAGCTGCATACCGATCAGAAAGCTACCCGTCATGCCACCA
TGCTAAACCCCTTGGCGCGCCGCGCCATCAGCGAACCTTTGGATGTGGGGGTTCGTGCCATCAACGCCATGTTGACCGTG
GGTAAGGGCCAACGTATGGGTCTATTTGCCGGTTCGGGTGTGGGTAAGAGTGTGCTGCTGGGCATGATGACCCGGGGCAC
CACGGCCGATGTGATTGTCGTTGGTCTGGTGGGGGAACGTGGCCGCGAAGTGAAAGAGTTTATCGAAGAGATCTTGGGCG
CCGAGGGACGTTCCCGCAGCGTGGTGGTGGCGGCGCCCGCCGATACCTCGCCGCTGATGCGTCTTCGAGCCTGTGAGACC
TCGACCCGTATCGCCGAATATTTTCGCGATCTGGGTTACAACGTATTGCTGCTGATGGACAGCCTGACTCGTTACGCCCA
GGCGCAGCGTGAGATCGCCCTGGCCGTCGGTGAGCCGCCGGCCACCAAAGGCTATCCGCCCTCTGTGTTTGCCAAGCTGC
CTAAGCTGGTGGAGCGCGCCGGTAACGGCGGCGCCGGCCAGGGCTCAATTACCGCCTTCTATACCGTGCTGACCGAGGGC
GATGATCTGCAGGATCCGATCGCCGATGCGGCGCGGGCGATCCTCGATGGCCATATTGTGTTATCGCGGGCATTAGCCGA
TTCGGGTCATTACCCTGCCATCGACGTCGAGGCCTCTATCAGCCGTGTGGCGCCTATGGTGATCAGTGACGCTCATCTGG
AGGCGACGCGCCGGGTGAAGCAGATGTATTCTCTGTACCAACAAAATCGCGACCTCATCTCTATCGGTGCCTATACTCAG
GGGAGCGACCCTCGTATCGATAACGCCATACGGCTGCAGCCAGCCATGAACGCCTTCCTGCGCCAGACCATGAAGGAAGC
CATCAGCTTCGATTCCAGCGCCTTGATGCTGGGTCAATTGAGCGCCCAGTGTCAGGTCTAG

Protein sequence :
MKTRQNRLLDKLRQHTQAVAPFMPEASGQLVRVVGLTLEATGCKAPVGSLCSIATMAGDLIAEVIGFDDKLLYLMPIEEL
SGVMPGAKVTPLGEQTGLNVGMGLLGRVLDGNGAPIDGLGQLHTDQKATRHATMLNPLARRAISEPLDVGVRAINAMLTV
GKGQRMGLFAGSGVGKSVLLGMMTRGTTADVIVVGLVGERGREVKEFIEEILGAEGRSRSVVVAAPADTSPLMRLRACET
STRIAEYFRDLGYNVLLLMDSLTRYAQAQREIALAVGEPPATKGYPPSVFAKLPKLVERAGNGGAGQGSITAFYTVLTEG
DDLQDPIADAARAILDGHIVLSRALADSGHYPAIDVEASISRVAPMVISDAHLEATRRVKQMYSLYQQNRDLISIGAYTQ
GSDPRIDNAIRLQPAMNAFLRQTMKEAISFDSSALMLGQLSAQCQV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-81 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 9e-82 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-82 49
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-82 49
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-82 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-81 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-82 49
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 3e-61 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-79 48
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-80 48
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-80 48
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-65 48
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 9e-66 48
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-64 47
lscN AAO18044.1 LscN Virulence TTSS locus Protein 7e-73 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-69 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-69 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 5e-69 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 4e-69 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-69 47
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-62 46
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-62 46
escN AAC38383.1 EscN Virulence LEE Protein 4e-70 46
escN AAL57542.1 EscN Virulence LEE Protein 2e-69 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-69 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 1e-69 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-69 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 8e-70 46
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-69 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 4e-70 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 4e-70 46
escN AAC31513.1 L0034 Virulence LEE Protein 3e-70 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 3e-70 46
escN AAK26715.1 EscN Virulence LEE Protein 2e-69 46
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-64 45
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-69 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-67 43
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-71 43
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-71 43
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-69 43
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-64 43
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-64 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 5e-64 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 6e-54 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_001093497.1 flagellum-specific ATP synthase VFG1254 Protein 3e-119 63
fliI YP_001093497.1 flagellum-specific ATP synthase VFG2331 Protein 5e-104 63
fliI YP_001093497.1 flagellum-specific ATP synthase VFG2499 Protein 5e-109 60
fliI YP_001093497.1 flagellum-specific ATP synthase VFG0048 Protein 5e-81 49
fliI YP_001093497.1 flagellum-specific ATP synthase VFG2144 Protein 7e-78 47
fliI YP_001093497.1 flagellum-specific ATP synthase VFG0515 Protein 3e-69 47
fliI YP_001093497.1 flagellum-specific ATP synthase VFG0192 Protein 5e-77 46
fliI YP_001093497.1 flagellum-specific ATP synthase VFG0730 Protein 2e-70 46
fliI YP_001093497.1 flagellum-specific ATP synthase VFG0812 Protein 2e-70 46
fliI YP_001093497.1 flagellum-specific ATP synthase VFG0390 Protein 2e-73 45
fliI YP_001093497.1 flagellum-specific ATP synthase VFG2459 Protein 3e-61 41