Gene Information

Name : lysS (Mjls_2998)
Accession : YP_001071268.1
Strain : Mycobacterium sp. JLS
Genome accession: NC_009077
Putative virulence/resistance : Unknown
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 3127563 - 3130898 bp
Length : 3336 bp
Strand : +
Note : catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA

DNA sequence :
ATGACGCTCACCAGTCCGCCGAGGACGCGCGCCGACTCCCGCCACTCCTGGGTTCCCGCGGCCGCCGGGTGGACCGTCGG
CGTCATCGCCACCCTCTCGCTCATCGCGAGCGTCTCACCGCTGGTCCGGTGGATCATCCGGGTGCCGCGAGAGTTCGTCA
ACGACTACATCTTCAACTTCCCCGACACCAGCTTCGCCTGGGCGTTCGTGCTGGCGCTGCTGGCGGGCGCGCTGGCCGCC
CGCAAACGCATCGCCTGGTGGATTCTGCTGCTGTACATGGTCGCCGCGGTCGGCTGGAACGTCGCCGATCTGCTGACCGG
TGACGAATCGGTCGTCGAGGAGATGGGCGAGGTCATCGGCCTCGCCTTCCACCTTGCGGCCGTGGCCTTCCTGCTGTTGG
CCCGCCCGCTGTTCTGGGCCCGCGTGCGGCGCGGCGCCCTGTTCAAGGCGGCCGGTGTGCTGGCCGCCGGAATGGCGGTC
GGCGTGCTCGTCGGCTGGGGTCTGCTCGAACTGTTCCCCGGCGACCTCGAACGCGACTACCGCTTGGCATACGCGGCGAA
CCGGGTGTTCGCGTTCGCCGGCGTCGACCCCGACGCGTTCGACGGACAACACCCACACGTGGTCGTCAACGCCCTGCTCG
GGTTGTTCGGCGCGCTGGCGTTGATGGCCGCGGCGATCGTGCTGTTCCAGTCGCAGCGCTCCGAGAACGCGCTCACCGGC
GAAGACGAATCGGCCATCCGCGGTCTGCTCGAGCTCTACGGCAAGAACGACTCGCTGGGGTATTTCGCCACCCGGCGGGA
CAAGTCCGTGGTGTTCGCACCGAACGGACGCGCCGCGATCACCTACCGGGTCGAGGTGGGGGTCTGCCTGGCCAGCGGTG
ACCCGGTCGGCGACCCGAAAGCCTGGCCGCAGGCGATCGACGCGTGGCTGGCGCTGTGCGGGACGTACGGCTGGGCACCG
GGTGTCATGGGTGCCAGCGTGGGCGGGGCCGAGGCGTTCCGGGCCGCCGGACTCAGCGCGATCCAACTGGGCGACGAGGC
CATCCTGCATCCGGACAGCTTCCGGCTCTCCGGGCCGGACATGCGGGCGGTGCGCCAGGCCGTCACGCGGGCCCGCCGCG
CCGGGGTGACCGTGCGGATCCGCAGACACCGGGAGCTCTCACCCGAACAGATGGCCGAGGTGATCGCCCACGCCGACGCC
TGGCGCGACACCGAGACAGAACGCGGCTTCTCGATGGCGCTCGGCCGCCTCGGCGATCCCGCCGACAGCGACTGCCTGCT
GGTGGAGGCCGTCCAGGGCGAGACCGGGGGCGAGCGAAGCGACCCGGGGAGTGGCACCGTCGTGGCCATGTTGTCGCTGG
TGCCGTGGGGCAGCAACGGCGCCTCCCTCGACGTGATGCGGCGTTCCCCGCAGTCACCCAACGGCACCATCGAGTTGATG
GTCAGCGAGCTGTGCATGCAGGCCGAGGACATCGGCGTCACCCGCATCTCCCTGAACTTCGCGATGTTCCGGTCGGCGTT
CGAGCAGGGCGCGCAACTCGGCGCCGGACCCGTGGCCCGGCTGTGGCGCTGGCTGCTGGTCTTCTTCTCCCGGTGGTGGC
AGCTGGAGACGCTGTACCGCTCGAACATGAAATACCAACCGCAGTGGGTGCCGCGCTACGCCTGCTACGAGGACGCGCGC
CTCATCCCCCGCGTCGGGGTGGCATCGGTGATCGCCGAGGGCTTCCTGGTGCTGCCGTTCTCGCGGCGCAACAAACAACA
CACCGGCCACCACACGTCCGCACCGCAGGACCTGGTCGCCAGCGGCGTGCTCCACCACGACGGCACCGCACCCGACATGT
CCGGGTTGCGCACCGACACCGCCGACGACGAACCGCCCCGGCTGCCCGAGCAGGTGCGGGTCCGGATGGCGAAACTCAAG
GCGCTGCAGGCCGACGGCGTCGACGCCTATCCGGTCGGCCGGCCACCCAGCCACACCGCGGCGGCTGCCGTCGACTCCCC
CGACGACGTCGAACTCGATGTCGCCGGCCGGGTGCTGCGCATCCGCGACTACGGCGGTGTGCTGTTCGCCCAGTTACGGG
ACTGGTCGGGTGAAGTGCAACTGCTGCTGGACAATTCGACACTCGAGCAGGGCAGCACCGCCGACTTCACCGCGGCCATC
GACCTCGGCGACCTCATCGAGGCGACCGGCACCATGGGCTACAGCAAGAACGGGACCCGCTCGCTGCTGGTGCGGCACTG
GCGGCTCACCGGTAAATGCCTGCGTCCCCTGCCGGACAAGTGGAAGGGCCTGACCGATCAGGAGGCCCGCGTCCGGGCCC
GCTACGTCGACCTGGCGGTCAACACCGAGGCGCGGGATCTGATCCGGGCCCGCAGCGGCGTGCTGCACGCGATCCGGGAC
ACGCTGTACCACAAGGGTTTCCTCGAGGTCGAGACCCCGATCCTGCAGCAGATCCACGGCGGCGCCAACGCACGGCCGTT
CCTGACCCACATCAACGCCTACGACCTGGACCTGTATCTGCGCATCGCGCCCGAGCTGTATCTCAAGCGGCTCTGCGTCG
GTGGGGTGGAGCGGGTCTTCGAACTCGGCCGCGCGTTCCGCAACGAAGGCGTCGACTTCTCCCACAACCCCGAATTCACA
CTGCTGGAGGCGTATCAGGCGCACGCCGACTACCACGTGTGGATCGACGGGTGCCGTGAACTCATCCAGAACGCCGCGAT
GGCCGCCAACGGTGAGCACGTGTTCCTCCGTCCGCGTGACGACGGCGTCCTCGAACCCGTCGACATCTCCGGACCGTGGA
CGGTCAAGACGGTGCACGATGCGGTGTCCGAGGCGCTCGGCGAACACATCGACGCCGCCACGGAACTGCCGACGCTGCGC
AAGCTGGCCGACGCGGCGGGCATCCCCTATCTGACCCACTGGGACGAGGGGGCGGTGGTGCTCGAGATGTACGAGCACCT
GGTCGAGGACCGCACCGAGAAGCCGACGTTCTACAAGGACTTCCCGACGTCCGTATCCCCCTTGACGCGCCCGCATCGCA
GCATTGCCGGCGTCGCCGAACGGTGGGACCTCGTCGCCTGGGGTGTCGAATTGGGCACCGCCTACAGCGAACTCACCGAT
CCGGTGGAGCAGCGTCGTCGACTGCAGGCGCAGTCGCTGCTCGCCGCGGGCGGTGATCCGGAGGCGATGGAACTCGACGA
GGACTTCCTGCAGGCCATGGAGTACGCGATGCCGCCCACCGGTGGGCTGGGCATGGGTGTCGATCGGGTGGTCATGCTGA
TCACCGGCCGCAGCATCCGGGAGACGCTTCCGTTCCCGTTGGCCAGACCACGTTGA

Protein sequence :
MTLTSPPRTRADSRHSWVPAAAGWTVGVIATLSLIASVSPLVRWIIRVPREFVNDYIFNFPDTSFAWAFVLALLAGALAA
RKRIAWWILLLYMVAAVGWNVADLLTGDESVVEEMGEVIGLAFHLAAVAFLLLARPLFWARVRRGALFKAAGVLAAGMAV
GVLVGWGLLELFPGDLERDYRLAYAANRVFAFAGVDPDAFDGQHPHVVVNALLGLFGALALMAAAIVLFQSQRSENALTG
EDESAIRGLLELYGKNDSLGYFATRRDKSVVFAPNGRAAITYRVEVGVCLASGDPVGDPKAWPQAIDAWLALCGTYGWAP
GVMGASVGGAEAFRAAGLSAIQLGDEAILHPDSFRLSGPDMRAVRQAVTRARRAGVTVRIRRHRELSPEQMAEVIAHADA
WRDTETERGFSMALGRLGDPADSDCLLVEAVQGETGGERSDPGSGTVVAMLSLVPWGSNGASLDVMRRSPQSPNGTIELM
VSELCMQAEDIGVTRISLNFAMFRSAFEQGAQLGAGPVARLWRWLLVFFSRWWQLETLYRSNMKYQPQWVPRYACYEDAR
LIPRVGVASVIAEGFLVLPFSRRNKQHTGHHTSAPQDLVASGVLHHDGTAPDMSGLRTDTADDEPPRLPEQVRVRMAKLK
ALQADGVDAYPVGRPPSHTAAAAVDSPDDVELDVAGRVLRIRDYGGVLFAQLRDWSGEVQLLLDNSTLEQGSTADFTAAI
DLGDLIEATGTMGYSKNGTRSLLVRHWRLTGKCLRPLPDKWKGLTDQEARVRARYVDLAVNTEARDLIRARSGVLHAIRD
TLYHKGFLEVETPILQQIHGGANARPFLTHINAYDLDLYLRIAPELYLKRLCVGGVERVFELGRAFRNEGVDFSHNPEFT
LLEAYQAHADYHVWIDGCRELIQNAAMAANGEHVFLRPRDDGVLEPVDISGPWTVKTVHDAVSEALGEHIDAATELPTLR
KLADAAGIPYLTHWDEGAVVLEMYEHLVEDRTEKPTFYKDFPTSVSPLTRPHRSIAGVAERWDLVAWGVELGTAYSELTD
PVEQRRRLQAQSLLAAGGDPEAMELDEDFLQAMEYAMPPTGGLGMGVDRVVMLITGRSIRETLPFPLARPR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lysS1 YP_005682181.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 4e-151 45
lysS1 YP_005684273.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 1e-151 45
lysX YP_003784331.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 1e-151 45
lysS1 YP_005686365.1 lysyl-tRNA synthetase Not tested PiCp 7 Protein 1e-151 45