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Name : lysS (APL_0809) Accession : YP_001053510.1 Strain : Actinobacillus pleuropneumoniae L20 Genome accession: NC_009053 Putative virulence/resistance : Virulence Product : lysyl-tRNA synthetase Function : - COG functional category : J : Translation, ribosomal structure and biogenesis COG ID : COG1190 EC number : 6.1.1.6 Position : 930728 - 932230 bp Length : 1503 bp Strand : - Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA DNA sequence : ATGTCCGAAGTTGAACATCAAGAATTAGATCTTAACGGTGAAATGTTAGCTCGCCGTGAGAAATTAGCAAAATTACGTGA ACAAGGTAATCCGTTCCCAAATACTTTCCGTCGTGATGCTTACGCAGAAAAATTACACGCACAATACGATGAAGTAGAGG GTGAAGCGTTAAAAGAACAAGATATTCAAGTGAAAGTTGCCGGTCGTATTATGCTTAAACGTGTAATGGGTAAGGCATCT TTCTTTACCATTCAAGATGTAAGCGGTCAAATCCAGCTTTATGTTGCACGTGATAACCTTGCGGAAGGTGTTTATGCGGA TAAAGTCAGTATGTGGGATTTAGGCGATATCGTCGGTGTTGCCGGTACGCTTTTCAAAACGAAAACGGGCGAATTAACCG TTCGTTGTTCGGAAGTGGAGTTATTAACTAAATCACTTCGTCCGTTACCAAATAAAGTACAAGGCTTAACCGACCAAGAA ACACGTTATCGTCAGCGCTATTTAGACTTAATTTCTAATGAAGAATCTCGCCGCACGTTTATGATTCGCTCAAAAGTGGT TTCAGGCATTCGTCAATTCTTCTTAGAGAAAGACTTTATTGAAGTTGAAACGCCAATGTTACAAGTGATTCCGGGCGGTG CGGCTGCAAAACCGTTTATCACTCACCATAATGCGTTAGACGTAGATATGTATTTACGTATCGCACCTGAGCTTTACTTA AAACGTTTAGTAGTTGGCGGTTTTGAGCGTGTATTCGAATTAAACCGTAACTTCCGTAATGAAGGTGTTTCAGTGCGTCA CAACCCTGAGTTCACTATGATCGAATACTATCAAGCGTATGCGGATTATCACGATTTAATGGATAACACCGAAGAATTAT TACGTAAATTAGCGATTGATATTCTTGGCACGACAACCGTTCCTTACGGTGAATACGTGTTCGATTTCGGTAAACCGTTT GAACGTATCACAATGCACGATGCAATCGTGAAATACGGTAACGGCATTACACGTGAAGATTTAGACAGCTTTGAAAAATC AGTAGAAATTGCAAAAGGCTTAGGTATTGAAATCCAAAAATCTTGGGGCTTAGGTTCTGTGGTTAATGCGATTTTTGAAG AAGTTGCGGAACATCAATTAATTCAACCGACCTTCTTAATGGCACATCCGGCAGAAATTTCACCGCTTGCGCGCCGTAAT GATGAAAACCCTGAAGTAACTGATCGTTTCGAATTATTTATCGGTGGTCGTGAAATCGGTAACGGTTTCTCAGAATTAAA TGATGCGGAAGACCAAGCAGAGCGTTTTGATGCACAAGTGGCAGCAAAAGATGCGGGTGATGATGAAGCGATGTTTAAAG ATGAAGACTTCGTTGTTGCGTTAGAACACGGTTTACCGCCGACAGCCGGCGAAGGTTTAGGTATCGACCGTTTAGCAATG ATTTTTGCAAACGCACCGTCAATTCGTGATGTGATCTTATTCCCTGCGATGCGTCAGAAATAA Protein sequence : MSEVEHQELDLNGEMLARREKLAKLREQGNPFPNTFRRDAYAEKLHAQYDEVEGEALKEQDIQVKVAGRIMLKRVMGKAS FFTIQDVSGQIQLYVARDNLAEGVYADKVSMWDLGDIVGVAGTLFKTKTGELTVRCSEVELLTKSLRPLPNKVQGLTDQE TRYRQRYLDLISNEESRRTFMIRSKVVSGIRQFFLEKDFIEVETPMLQVIPGGAAAKPFITHHNALDVDMYLRIAPELYL KRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEYYQAYADYHDLMDNTEELLRKLAIDILGTTTVPYGEYVFDFGKPF ERITMHDAIVKYGNGITREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAEISPLARRN DENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEAMFKDEDFVVALEHGLPPTAGEGLGIDRLAM IFANAPSIRDVILFPAMRQK |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| unnamed | CAD66193.1 | putative lysil-tRNA synthetase LysU | Not tested | PAI III 536 | Protein | 5e-150 | 62 |
| lysS | YP_005163386.1 | lysyl-tRNA synthetase | Not tested | Not named | Protein | 4e-87 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| lysS | YP_001053510.1 | lysyl-tRNA synthetase | VFG1668 | Protein | 3e-150 | 62 |
