Gene Information

Name : Memar_0527 (Memar_0527)
Accession : YP_001046442.1
Strain : Methanoculleus marisnigri JR1
Genome accession: NC_009051
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 509658 - 511157 bp
Length : 1500 bp
Strand : -
Note : catalyzes the formation of phosphoenolpyruvate from pyruvate

DNA sequence :
ATGATGCTCGAAGAGGTGCTGCAACGGATATCCGAGCAGGCGAAACGGAAGCGGATGAGCCTGCCCGACCATAAAACAAA
GATTGTCTGTACCATCGGGCCCGCATCGCGCTCCCGGGAGACGCTCTCCCGGATGCTCCTCGCCGGCATGAACGTCGCCC
GGCTCAATCTCTCCCACGGATCGTTCGACGAGCACCGCGAGAACATCCGAAACATCCGTACCGCCGCGGAGGATACGGGG
CTCCCGGTCACGATCCTGCTCGACCTGCCGGGGCCGAAGATCCGGATCGGCGATCTCGCATACGAGCCCCTGGAACTCCA
CAAAGGTGAGACCGTCACCCTCACGACCACCCCGGCGTCGAGCGATCCATCCCGGATCCCCGTCGACTTCGACCAGTTCC
CGCAGCTGGTCTCGGAGGGGAGCACCATCTTCCTCAACGACGGGTTCCTCCAGCTCCAGGTCGATGAGGTTGCCGAGAAC
GAGGTCCGGGCGACGGTGGTCATCGGCGGCCCCCTCCTCTCGCGCAAGGGGATGAGTCTCGTCGGCGGCGGGGGGATCGT
CGCCCTCAGCGCCGTGACCGACCGGGACCTCGAGTGCATCGAGTTCGGGCTTGCCGAAGGGCTTGACACCTTCAGTATCT
CCTTCATCGAGCGGGCGGAGGACATCCTGAAAGCACGGGAGTATGCCGCCCGATCCGGGAAGTCCATCAAAGTGATCGCC
AAGATCGAGCGGCAGGAAGCGCTCGCAAACCTCGACGAGATCCTCCGGGAGACCGACGGGGTCATGATCGCCAGGGGCGA
TCTCGGTGTCCAGACACCCATCGAGGAGGTCCCCGCGGTGCAGAAGCGGATCATCCAGAAAGCCAACATTCTCGGCCGGC
CGGCGATCACCGCCACCCAGATGCTGATGTCGATGACCGACAACATCCGGCCCACCCGGGCGGAGGTGACCGACGTCGCG
AACGCCATCCTCGACGGCACCGACGCTGTCATGCTCTCCGAGGAGACGTCGATCGGGAAGTACCCGGTCGAGACGGTCGC
AATGATGGCGAAAATCGCCCGGTCGACCGAGGAGAAGCGCTCCCTTGTCGGGGCCGGGTGCAGCCCGCTCGACTACTTCC
GGCGCGGAAAGGGGCGCGAGGAGATCACCGTCAACGACGTCGTCTCGCTAAACGTCATCGAGGCGCTGGACGCGCTCGGC
ATCCACTACGTGCTGACCCCCACCCGCTCCGGGAGCACCCCGAGGAACATCTCCCGGTTCAAGCCCGGGGCGTGGATCCT
CGCGTTCACCCGGAACCCGAGCACCTTCAAGTTCCTGGCGTTCTCCTACGGGGTAAGCCCGTTCCTGATCCGGAGCGAGA
AGGAGTACTGGCACGGGGCGATCCTCGACTCGATCCGGGACTCGGGGTTGATCTCGCCCGGTGAGCGCGTGGTGCTGACC
GAGGGGGTATCTCCCGGACGCGAGGGGACCGATTCGCTCATCATCCTCACGGTCGATTGA

Protein sequence :
MMLEEVLQRISEQAKRKRMSLPDHKTKIVCTIGPASRSRETLSRMLLAGMNVARLNLSHGSFDEHRENIRNIRTAAEDTG
LPVTILLDLPGPKIRIGDLAYEPLELHKGETVTLTTTPASSDPSRIPVDFDQFPQLVSEGSTIFLNDGFLQLQVDEVAEN
EVRATVVIGGPLLSRKGMSLVGGGGIVALSAVTDRDLECIEFGLAEGLDTFSISFIERAEDILKAREYAARSGKSIKVIA
KIERQEALANLDEILRETDGVMIARGDLGVQTPIEEVPAVQKRIIQKANILGRPAITATQMLMSMTDNIRPTRAEVTDVA
NAILDGTDAVMLSEETSIGKYPVETVAMMAKIARSTEEKRSLVGAGCSPLDYFRRGKGREEITVNDVVSLNVIEALDALG
IHYVLTPTRSGSTPRNISRFKPGAWILAFTRNPSTFKFLAFSYGVSPFLIRSEKEYWHGAILDSIRDSGLISPGERVVLT
EGVSPGREGTDSLIILTVD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-68 41
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 7e-68 41
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 6e-68 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-68 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Memar_0527 YP_001046442.1 pyruvate kinase VFG0479 Protein 1e-68 41