Gene Information

Name : lysS (Mpe_A0600)
Accession : YP_001019797.1
Strain : Methylibium petroleiphilum PM1
Genome accession: NC_008825
Putative virulence/resistance : Virulence
Product : lysyl-tRNA synthetase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG1190
EC number : 6.1.1.6
Position : 631016 - 632542 bp
Length : 1527 bp
Strand : -
Note : class II; LysRS2; catalyzes a two-step reaction, first charging a lysine molecule by linking its carboxyl group to the alpha-phosphate of ATP, followed by transfer of the aminoacyl-adenylate to its tRNA; in Methanosarcina barkeri, LysRS2 charges both tRNA

DNA sequence :
ATGACCACCACGCCCCCGAACGCCGCCACGCCCGGCACCGACGACAACCAGCTGATCGCCGAACGCCGCGAGAAGCTCGC
TGCACTGCGTGCGCAGGCCCAGGCCACGGGGAGAGCCGCCTTCCCCAATGACTTCAAGCCGGGCGACCATGCCGTCGACC
TGCACCGCCTGCACGAGGGCAAGAGCGCCGAGGAGCTGGAGGCGCTGGCCATCCCGGCCCGCATCGCCGGCCGCATGATG
CTCAAGCGCGTGATGGGCAAGGCCTGCTTCGCGACGCTGCAGGACGGCTCGGGCCGCATCCAGCTCTACATCACGTCCGA
CGCCGTCGGCCCGGAGGCGCTGGCCGCCTTCAAGCACTGGGACCTGGGCGACATCCTCGGCGCCGAGGGCACGCTGTTCA
AGACCCGCACCGGCGAGCTGAGCCTCAAGGTCGGCACGATCCGGCTGCTGACCAAGAGCCTGCGGCCACTGCCCGACAAG
TTCCACGGCATGGCCGACCAGGAGCTCAAGTACCGCCAGCGCGAGGTCGACCTGATCATGGACGAGGCCTCGCGCGAGCG
CTTCCGCGCGCGCAGCAAGGCCGTGAGCTCGATCCGGCAGTTCATGGTCGAGCACGGCTTCCTCGAGGTCGAGACGCCGA
TGCTGCACCCCATCCCCGGCGGCGCGAACGCCAAGCCCTTCGTGACGCACCACAACGCGCTCGACCAGCAGATGTTCCTG
CGCATCGCGCCCGAGCTCTACCTGAAGCGGCTGGTGGTGGGCGGCTTCGAGCGGGTGTTCGAGATCAACCGCAACTTCCG
CAACGAGGGCGTGTCGGTCCGGCACAACCCCGAGTTCACGATGATGGAGTTCTACGCGGCCTACTGGACCCACCACGACC
TGATGGACTTCACCGAGCAGATCCTGCGCCACGCCGCGCGCAAGGCGGTGGGCACGGCGCAGCTCGCCTACGGTGGGCAG
GCCGTCGACCTGGAATCGCCGTTCGCGCGGCTCTCGGTGCGCGACTCGCTGATCGCCCATGCAGGATTGAGCGCGGCGGA
AGCCGCCGACACCACGGTGCTGCGCGCGAAGCTCGAAGCGCATGGCGAGAAGGCTCCGGCGCACTGGTCGCTCGCCGAAC
TGCAGTTCGGCCTGTTCGAGGCGGCCGTCGAGGAAAAGCTGTGGCAGCCGACCTTCATCGTCGACTACCCGGTGGAGGTG
TCGCCGCTGGCCCGCGCCTCCGATGCCGACCCGACGATCACCGAGCGCTTCGAGCTCTTCATCACCGGCCGCGAGTTCGG
CAACGGCTTCTCGGAGCTCAACGACCCCGAGGACCAGGCCGCGCGCTTCGCGGCCCAGGCCTCGAACAAGGACGCCGGTG
ACGAGGAGGCGATGTACTTCGACACCGACTACATCCGCGCGCTCGAGCACGGCCTGCCGCCCACCGGCGGCTGCGGCATC
GGCATCGACCGACTGATGATGCTGTTGACCGACTCGCCGAACATCCGCGACGTGATCCTGTTCCCCGCGCTGCGGCGCGA
GGGCTGA

Protein sequence :
MTTTPPNAATPGTDDNQLIAERREKLAALRAQAQATGRAAFPNDFKPGDHAVDLHRLHEGKSAEELEALAIPARIAGRMM
LKRVMGKACFATLQDGSGRIQLYITSDAVGPEALAAFKHWDLGDILGAEGTLFKTRTGELSLKVGTIRLLTKSLRPLPDK
FHGMADQELKYRQREVDLIMDEASRERFRARSKAVSSIRQFMVEHGFLEVETPMLHPIPGGANAKPFVTHHNALDQQMFL
RIAPELYLKRLVVGGFERVFEINRNFRNEGVSVRHNPEFTMMEFYAAYWTHHDLMDFTEQILRHAARKAVGTAQLAYGGQ
AVDLESPFARLSVRDSLIAHAGLSAAEAADTTVLRAKLEAHGEKAPAHWSLAELQFGLFEAAVEEKLWQPTFIVDYPVEV
SPLARASDADPTITERFELFITGREFGNGFSELNDPEDQAARFAAQASNKDAGDEEAMYFDTDYIRALEHGLPPTGGCGI
GIDRLMMLLTDSPNIRDVILFPALRREG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed CAD66193.1 putative lysil-tRNA synthetase LysU Not tested PAI III 536 Protein 1e-130 55
lysS YP_005163386.1 lysyl-tRNA synthetase Not tested Not named Protein 6e-79 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
lysS YP_001019797.1 lysyl-tRNA synthetase VFG1668 Protein 6e-131 55